GC–MS analysis was carried out using 7890A GC (Agilent Technologies, United States) equipped with an HP-5MS column (30 m × 0.25 mm/0.25 μm, Agilent Co., United States), which was directly connected to a triple-axis HED-EM 5975C mass spectrometer (Agilent Co., United States). The injection volume was 1 μl with flow mode in split control. The carrier gas flow was set at 1 ml min–1 helium. The oven temperature was initially held at 40°C for 2 min. Thereafter, the temperature was raised with a gradient of 3°C min–1 until the temperature reached 130°C and held for 2 min. Again, the temperature was raised with a gradient of 5°C min–1 up to 220°C and held for 1 min. Finally, the oven temperature was raised to 280°C with an increment of 10°C min–1. The total runtime was 59 min. The MS acquisition parameters were set with the ion source temperature 175°C, electron ionization 70 eV, full scan mode (50–550 mass units), and transfer line temperature 250°C. Compounds were identified by matching their mass spectra. Volatile organic compounds (VOCs) were identified by library matching from the National Institute of Standards and Technologies Mass Spectra Library (Saha et al., 2015a ).
Hed em 5975c mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The HED-EM 5975C mass spectrometer is a laboratory instrument designed for the detection and identification of chemical compounds. It utilizes electron ionization to generate ions from the sample, which are then separated and detected based on their mass-to-charge ratio. The core function of this device is to provide analytical data about the composition and structure of chemical samples.
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Lab products found in correlation
4 protocols using hed em 5975c mass spectrometer
1
GC-MS Analysis of Volatile Compounds
2
Clove Oil Analysis by GC-MS
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The impact of CuNPs on EO (clove oil) was studied using GC-MS (Agilent Technologies, 8010C GC, USA) outfitted with an HP-5MS column (30 m × 0.25 mm; 0.25 mm) (Agilent Co., USA) coupled to a triple axis HED-EM 5975C mass spectrometer. Flow mode in split control was used to inject a volume of 2 μL at a ratio of 1:10. At a head pressure of 7.5 psi, the carrier gas flow was set at 0.75 mL min−1 helium (High purity >99.99%, New Delhi, India). The oven's temperature was initially set at 40°C for 1 min and then was raised with a gradient of 3°C min−1 to reach 60°C and held for 2 min. Furthermore, a 5°C min−1 gradient was used to bring the temperature to 220°C. Finally, the temperature increased to 300°C with an increment of 10°C min−1. The MS acquisition parameters were as follows: transfer line temperature 250°C, ion source 200°C, electron ionization 70 eV, full scan mode (50–550 mass units), and E.M voltage 1,220 V. The scan time was 1 s, and the compounds were identified by matching their respective mass spectra with the reference from the NIST (National Institute of Standards and Technologies) Mass Spectra Library.
3
GC-MS Characterization of Essential Oils
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Essential oils were characterized by gas chromatography–mass spectrometry (GC-MS) (7890A GC, Agilent Technologies equipped with an HP-5MS column (30 m × 0.25 mm × 0.25 μm, Agilent Co., CA, USA), connected to a triple-axis HED-EM 5975C mass spectrometer. Carrier gas flow was 1 ml min−1 and the injection volume was 1 μl. Helium was used as carrier gas at a head pressure of 10 psi. In GC, the oven temperature was initially held at 40°C for 1 min, and thereafter the temperature was raised with a gradient of 3°C min−1 until the temperature reached 220°C. Other settings include 250°C interface temperature and ion source temperature of 200°C.
4
GC-MS Analysis of Essential Oil Composition
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The essential oil was analyzed by GC-MS (7890A GC, Agilent Co., USA), and its chemical composition was determined using an HP-5MS column (30 m × 0.25 mm; 0.25 µm; Agilent Co., USA). The instrument is directly connected to a triple-axis HED-EM 5975C Mass Spectrometer (Agilent Co., USA) with a split control inflow mode enabled with 1 µl of injection volume. Helium (high purity, New Delhi, India) with a head pressure of 10 psi is used as the carrier gas with a flow rate of 1 ml min−1. The oven temperature was initially raised to 60˚C for 1 min and gradually increased to the interface temperature of 250˚C maintaining a gradient of 4˚C min−1. The ion source temperature was retained at 200˚C and electron impact ionization (EI) was performed at 70 eV full scan mode and selected ion monitoring (SIM) mode was implemented for mass spectra analysis (37 (link), 38 (link)).
The MSD productivity Chemstation program was used to obtain the pure spectrum by processing the raw MS data and excluding the residual background contaminants, column bleed, and partially eluted peaks (Figure S1 Table S1
The MSD productivity Chemstation program was used to obtain the pure spectrum by processing the raw MS data and excluding the residual background contaminants, column bleed, and partially eluted peaks (
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