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Tnp atp triethylammonium salt

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TNP-ATP triethylammonium salt is a laboratory reagent used in various biochemical and biophysical applications. This compound serves as a fluorescent ATP analog, allowing for the study of ATP-dependent processes and the detection of ATP-binding proteins. The triethylammonium salt formulation provides solubility in aqueous solutions.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (4 mentions) Prazosin hydrochloride, by Merck Group (2 mentions) Yohimbine hydrochloride, by Bio-Techne (2 mentions) Tetrodotoxin ttx, by Bio-Techne (2 mentions) L norepinephrine bitartrate salt monohydrate ne, by Merck Group (2 mentions) R phenylephrine hydrochloride, by Merck Group (2 mentions) Pyridoxalphosphate 6 azophenyl 2 4 disulfonic acid tetrasodium salt ppads, by Bio-Techne (2 mentions) Picrotoxin ptx, by Bio-Techne (2 mentions) 10panx, by Bio-Techne (2 mentions) Apyrase, by Merck Group (2 mentions) Brilliant blue g, by Merck Group (2 mentions) Epilife defined growth supplement, by Thermo Fisher Scientific (2 mentions) Epilife culture medium with ca2 60 μm, by Thermo Fisher Scientific (2 mentions) Suramin hexasodium salt, by Bio-Techne (2 mentions) Ppads tetrasodium salt, by Bio-Techne (2 mentions) Nf157, by Bio-Techne (2 mentions) Arl67156, by Bio-Techne (2 mentions) Adenosine 5 γ thio triphosphate tetralithium salt, by Merck Group (2 mentions) On targetplus sirna, by GE Healthcare (2 mentions)

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TNP-ATP (7 citations)

6 protocols using tnp atp triethylammonium salt

1

Pharmacological Manipulation of Neurochemicals

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The following drugs were kept at −20°C as stock solutions and dissolved in aCSF to their final concentrations on the day of experiments: L-(−)-norepinephrine (+)-bitartrate salt monohydrate (NE, 10-100 μM, Sigma-Aldrich, shielded from light exposure during preparation, storage and application), picrotoxin (PTX, 50 μM, Tocris), prazosin hydrochloride (10 μM, Sigma-Aldrich), (R)-(−)-phenylephrine hydrochloride (100 μM, Sigma-Aldrich), yohimbine hydrochloride (20 μM, Tocris), tetrodotoxin (TTX, 1 μM, Tocris), TNP-ATP triethylammonium salt (10 μM, Tocris), pyridoxalphosphate-6-azophenyl-2′,4’-disulfonic acid tetrasodium salt (PPADS, 100 μM, Tocris).
Fluorocitric acid (FCA, Sigma-Aldrich) was prepared on the day of experiments with DL-fluorocitric acid barium salt (Paulsen et al., 1987 (link)) dissolved in 1 mL of 0.1 M HCl. Two-to-three drops of 0.1 M Na2SO4 was added to precipitate the Ba2+, after which 2 mL of 0.1 M Na2HPO4 was added and the suspension was centrifuged at 1000 g for 5 min. The supernatant was added to the aCSF to achieve a concentration of 100 μM.
Chen C., Jiang Z., Fu X., Yu D., Huang H, & Tasker J.G. (2019). Astrocytes Amplify Neuronal Dendritic Volume Transmission Stimulated by Norepinephrine. Cell reports, 29(13), 4349-4361.e4.
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2

Pharmacological inhibition of purinergic signaling

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EpiLife culture medium with Ca2+ (60 μM), EpiLife defined growth supplement (EDGS), penicillin/streptomycin, and Lipofectamine 2000 reagent were purchased from Thermo Fisher Scientific (Waltham, MA USA). On-target plus siRNA was purchased from GE healthcare (Little Chalfont, UK). FNC coating mixture was purchased from Athena ES (Baltimore, MA, USA). Suramin hexasodium salt (an inhibitor of P2X and P2Y receptors), PPADS tetrasodium salt (an inhibitor of P2X and P2Y receptors), TNP-ATP triethylammonium salt (an inhibitor of P2X receptor), NF 157 (an inhibitor of P2Y11 and P2X1), ARL 67156 (an inhibitor of ecto-ATPases), and 10Panx (an inhibitor of Pannexin-1 channel) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Adenosine-5’-(γ-thio)-triphosphate tetralithium salt (ATPγS, a non-hydrolyzable ATP analogue), Brilliant blue G (BBG; an inhibitor of P2X receptors), and apyrase were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Nakajima K.I., Tatsumi M, & Zhao M. (2019). An Essential and Synergistic Role of Purinergic Signaling in Guided Migration of Corneal Epithelial Cells in Physiological Electric Fields. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 52(2), 198-211.
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3

Pharmacological Manipulation of Neurochemicals

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following drugs were kept at −20°C as stock solutions and dissolved in aCSF to their final concentrations on the day of experiments: L-(−)-norepinephrine (+)-bitartrate salt monohydrate (NE, 10-100 μM, Sigma-Aldrich, shielded from light exposure during preparation, storage and application), picrotoxin (PTX, 50 μM, Tocris), prazosin hydrochloride (10 μM, Sigma-Aldrich), (R)-(−)-phenylephrine hydrochloride (100 μM, Sigma-Aldrich), yohimbine hydrochloride (20 μM, Tocris), tetrodotoxin (TTX, 1 μM, Tocris), TNP-ATP triethylammonium salt (10 μM, Tocris), pyridoxalphosphate-6-azophenyl-2′,4’-disulfonic acid tetrasodium salt (PPADS, 100 μM, Tocris).
Fluorocitric acid (FCA, Sigma-Aldrich) was prepared on the day of experiments with DL-fluorocitric acid barium salt (Paulsen et al., 1987 (link)) dissolved in 1 mL of 0.1 M HCl. Two-to-three drops of 0.1 M Na2SO4 was added to precipitate the Ba2+, after which 2 mL of 0.1 M Na2HPO4 was added and the suspension was centrifuged at 1000 g for 5 min. The supernatant was added to the aCSF to achieve a concentration of 100 μM.
Chen C., Jiang Z., Fu X., Yu D., Huang H, & Tasker J.G. (2019). Astrocytes Amplify Neuronal Dendritic Volume Transmission Stimulated by Norepinephrine. Cell reports, 29(13), 4349-4361.e4.
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4

Expression and Purification of P2X Receptors

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Unless otherwise stated, all compounds were purchased from Sigma-Aldrich (USA). TNP-ATP triethylammonium salt was purchased from Tocris bioscience (Catalog No.: 2464), with a purity of more than 98%. hP2X3 plasmid was purchased from Open Biosystems; ckP2X7 cDNA was a generous gift of Dr. Osamu Nureki and was subcloned into pcDNA3.1 vector; pcDNA3-rP2X2, pcDNA3-rP2X3 plasmids were generous gifts of Drs. Alan North and Linhua Jiang. All mutants were created using the KOD-Plus- mutagenesis kit (Toyobo, SMK-101) and confirmed by DNA sequencing [39] .
Ma X.B., Yue C.X., Liu Y., Yang Y., Wang J., Yang X.N., Huang L.D., Zhu M.X., Hattori M., Li C.Z., Yu Y, & Guo C.R. (2023). A shared mechanism for TNP-ATP recognition by members of the P2X receptor family. Computational and Structural Biotechnology Journal, 23, 295-308.
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5

Pharmacological inhibition of purinergic signaling

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EpiLife culture medium with Ca2+ (60 μM), EpiLife defined growth supplement (EDGS), penicillin/streptomycin, and Lipofectamine 2000 reagent were purchased from Thermo Fisher Scientific (Waltham, MA USA). On-target plus siRNA was purchased from GE healthcare (Little Chalfont, UK). FNC coating mixture was purchased from Athena ES (Baltimore, MA, USA). Suramin hexasodium salt (an inhibitor of P2X and P2Y receptors), PPADS tetrasodium salt (an inhibitor of P2X and P2Y receptors), TNP-ATP triethylammonium salt (an inhibitor of P2X receptor), NF 157 (an inhibitor of P2Y11 and P2X1), ARL 67156 (an inhibitor of ecto-ATPases), and 10Panx (an inhibitor of Pannexin-1 channel) were purchased from Tocris Bioscience (Minneapolis, MN, USA). Adenosine-5’-(γ-thio)-triphosphate tetralithium salt (ATPγS, a non-hydrolyzable ATP analogue), Brilliant blue G (BBG; an inhibitor of P2X receptors), and apyrase were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Nakajima K.I., Tatsumi M, & Zhao M. (2019). An Essential and Synergistic Role of Purinergic Signaling in Guided Migration of Corneal Epithelial Cells in Physiological Electric Fields. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 52(2), 198-211.
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6

Fibronectin and Epigenetic Regulation

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Fibronectin, 5-aza-2′-deoxycytidine, and TGFβ-1 humankineTM were purchased from Sigma. Collagen IV was purchased from Cultrex (Gaithersburg, MD). TNP-ATP triethylammonium salt was purchased from Tocris Bioscience (Bristol, UK).
Ghalali A., Ye Z.W., Högberg J, & Stenius U. (2014). Phosphatase and Tensin Homolog Deleted on Chromosome 10 (PTEN) and PH Domain and Leucine-rich Repeat Phosphatase Cross-talk (PHLPP) in Cancer Cells and in Transforming Growth Factor β-Activated Stem Cells. The Journal of Biological Chemistry, 289(17), 11601-11615.
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