Drug loading and drug release were measured using an Agilent 1200 Series HPLC system, which consisted of a quaternary pump, online degasser, manual injector with a 20-µl sample loop and Agilent Chemstation LC 3D software. Chromatography was conducted in reverse mode using acetonitrile and phosphate buffer (0.05 M, pH 5.65). A gradient at 40% for 1 min, raised to 60% in 14 min, was reduced to 40% in 1 min and held at 40% for 4 min. Total analysis time was 20 min. Mobile phase flow rate was 1.5 ml/min, with a column temperature at 30 °C and a sample injection volume of 5 µl. Detection was performed with a diode array detector at 240 nm for RTV and EFV. The drug concentration was obtained from calibration curves for RTV and EFV.
Chemstation lc 3d software
Manufactured by Agilent Technologies
Chemstation LC 3D software is a data analysis and instrument control software for liquid chromatography (LC) systems. It provides tools for data acquisition, processing, and reporting. The software supports 3D data visualization and comprehensive data analysis.
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Lab products found in correlation
2 protocols using chemstation lc 3d software
1
HPLC Analysis of Drug Loading and Release
2
Size-Exclusion Chromatography for Protein Analysis
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Size-exclusion chromatography (SEC) was performed under isocratic elution conditions with a HiLoad 16/600 Superdex 200 preparative grade column (10,000–600,000 relative molecular mass (Mr) range, GE Healthcare, Chicago, IL, USA) and a HiLoad 16/600 Superdex 75 preparative grade column (3,000–70,000 Mr range, GE Healthcare) for the analysis of SDP and EHPP, respectively, using an HP 1100 series liquid chromatograph with an ultraviolet absorption diode array detector (Agilent Technologies, Waldbronn, Germany). Instrument control, data acquisition and data processing were performed using the Chemstation LC3D software (Agilent Technologies). The mobile phase consisted of phosphate buffered saline (PBS, pH = 7.50) and absorbance was monitored at 214 nm. After column equilibration with mobile phase (two column volumes), a calibration curve was constructed by injecting 1 mL of a 500 mg/L mixture of protein standards of known Mr according to the manufacturer instructions. SDP and EHPP were analyzed by injecting volumes of 1 mL, and fractions (for EHPP only) were collected using an Agilent 1200 series analytical fraction collector (Agilent Technologies).
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