Sr11302

Manufactured by Bio-Techne

Sourced in United Kingdom, United States

SR11302 is a temperature control module designed for use with Bio-Techne's SR Series of stirrer hotplates. This module allows for precise temperature regulation of samples during experiments or processing.

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Close spelling variants of this product

AP-1 inhibitor SR11302 (3 citations)

29 protocols using sr11302

Vipera lebetina turanica Venom Toxin Analysis

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Snake venom toxin from Vipera lebetina turanica was purchased from Sigma (St. Louis, MO). SR11302 was purchased from Tocris (Bristol, UK).

Lee H.L., Park M.H., Son D.J., Song H.S., Kim J.H., Ko S.C., Song M.J., Lee W.H., Yoon J.H., Ham Y.W., Han S.B, & Hong J.T. (2015). Anti-cancer effect of snake venom toxin through down regulation of AP-1 mediated PRDX6 expression. Oncotarget, 6(26), 22139-22151.

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Modulating Retinal Angiogenesis in Mice

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All animal studies were performed according to protocols approved by the Institutional Animal Care and Use Committee at the Boston Children’s Hospital. Mice were housed under specific pathogen–free conditions. Vldlr^+/− (heterozygous) mice from The Jackson Laboratory (stock no. 002529) were crossed to generate homozygous and WT littermates. SR11302 (cat. 2476; Tocris Bioscience) was dissolved in corn oil. Vldlr^−/− pups were orally gavaged with SR11302 or vehicle control (corn oil) at two doses (low dose 0.5 mg/kg body weight and high dose 1 mg/kg body weight) daily from P5 to P15. P16 retinas were collected for PCR and neovascularization analysis.

Sun Y., Lin Z., Liu C.H., Gong Y., Liegl R., Fredrick T.W., Meng S.S., Burnim S.B., Wang Z., Akula J.D., Pu W.T., Chen J, & Smith L.E. (2017). Inflammatory signals from photoreceptor modulate pathological retinal angiogenesis via c-Fos. The Journal of Experimental Medicine, 214(6), 1753-1767.

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Antibody Validation for Western Blotting and IHC

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The following primary antibodies were used in the present study: Rabbit polyclonal anti-TRPM7 (cat. no. ab23455) was purchased from Abcam. Rabbit polyclonal anti-β-actin antibody (cat. no. A3854) was purchased from Sigma-Aldrich; Merck KGaA. Rabbit polyclonal anti-phosphorylated STAT3 (Y705) antibody (cat. no. 9131) was purchased from Cell Signaling Technology, Inc. Rabbit polyclonal PE anti-human CD133 antibody (cat no. 372804) and PE mouse IgG1, κ isotype ctrl (FC) antibody were purchased from BioLegend. Rabbit polyclonal anti-aldehyde dehydrogenase 1 antibody (ALDH1; cat no. GTX123973) was purchased from GeneTex, Inc. Mouse monoclonal anti-FOSL1 antibody (cat. no. sc-283107) was purchased from Santa Cruz Biotechnology, Inc for Western blotting, and rabbit polyclonal anti-FOSL1 antibody was purchased from Sigma-Aldrich (cat. no. SAB2108461) for Immunohistochemistry (IHC) staining. All secondary antibodies (goat anti-rabbit, peroxidase-conjugated, cat. no. AP132P; and goat anti-mouse antibody, peroxidase-conjugated, cat. no. AP124P) used for Western blotting were purchased from Calbiochem; Merck KGaA. STAT3 inhibitor XIII, C188-9, was purchased from EMD Millipore (cat. no. 573128). SR11302 was purchased from TOCRIS (cat. no. 2476).

Guo S., Ramar V., Guo A.A., Saafir T., Akpobiyeri H., Hudson B., Li J, & Liu M. (2023). TRPM7 transactivates the FOSL1 gene through STAT3 and enhances glioma stemness. Cellular and Molecular Life Sciences, 80(9), 270.

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Immunological Assay Protocol

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Antibodies are listed in SI Appendix, Table S1. Purchases include 740 Y-P, SC-10, SC-79, SB202190, U0126, and SR11302 from Tocris Bioscience; asiatic acid from Cayman Chemical; Ikk 16 from Calbiochem; CTLA4-Ig (Abatacept [Orencia]) from Ono Pharm Co.; and OVA (323 to 339) peptides from MBL. Recombinant mouse IL-4, IL-6, IL-12, and TNF-α were purchased from PeproTech. Mouse CD4 T Lymphocyte Enrichment Set (BD) was used for the enrichment of CD4⁺ T cells, and Cell Stimulation Mixture (plus protein transport inhibitors) (eBioscience) for intracellular cytokine staining.

Mikami N., Kawakami R., Chen K.Y., Sugimoto A., Ohkura N, & Sakaguchi S. (2020). Epigenetic conversion of conventional T cells into regulatory T cells by CD28 signal deprivation. Proceedings of the National Academy of Sciences of the United States of America, 117(22), 12258-12268.

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Inhibitor Compounds for Cell Signaling

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The PRSS3 inhibitor diminazene (#18678‐1) was obtained from Cayman Chemical (USA). The AP‐1 inhibitor T5224 (#S8966), MEK inhibitor trametinib (#S2673), and PI3K inhibitor dactolisib (#S1009) were purchased from Selleckchem (USA). The JNK inhibitor SP600125 (#420119) and p38 inhibitor SB202190 (#559388) were obtained from Calbiochem (Germany). Gα_i protein inhibitor PTX (#sc‐200837) was purchased from Santa Cruz (USA). Another AP‐1 inhibitor SR11302 (#2476), PAR2‐AP (SLIGKV‐NH2; #3010), and the control peptide (VKGILS‐NH2; #3392) were obtained from Tocris Bioscience (UK).

Zhou M., Li K, & Luo K.Q. (2023). Shear Stress Drives the Cleavage Activation of Protease‐Activated Receptor 2 by PRSS3/Mesotrypsin to Promote Invasion and Metastasis of Circulating Lung Cancer Cells. Advanced Science, 10(25), 2301059.

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Signaling Pathway Inhibitors in Cell Assays

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W 123 (10 µM), JTE 013 (1–10 µM), CAY 10444 (10 µM), specific inhibitors of S1PR1, S1PR2, S1PR3 respectively, S1P (0.1–10 µM), and the EGFR inhibitor tryphostin AG-1478 (0.3–3 µM) were all obtained from Cayman Chemical (Ann Arbor, MI, USA). Helenalin (1 µM), inhibitor of NF-κB, pEGFR antibody (p-Tyr-845 SC-23420-R) and total EGFR antibody (SC-03) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). SR 11302 (1 µM), inhibitor of activator protein-1 (AP-1) and SEW 2871 (10 µm), agonist of S1PR1 were obtained from Tocris Bioscience (Bristol, UK). Dichlorodihydrofluorescein diacetate (DCFH-DA) (10 µM) and N-acetyl cysteine (NAC) (1 mM) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Luciferase reporter lysis buffer was obtained from Promega (Madison, WI, USA). GM6001 (25 µM), the broad-spectrum hydroxamic acid inhibitor of matrix metalloproteinases (MMPs) and TAPI-1 (10 µM), inhibitor or MMPs and tumor necrosis factor-α converting enzyme (TACE) were obtained from Calbiochem (La Jolla, CA). Fura-2 AM (10 µM), and pluronic F127 (0.02%) were obtained from Life Technologies (Carlsbad, Ca).

O’Sullivan M.J., Hirota N, & Martin J.G. (2014). Sphingosine 1-Phosphate (S1P) Induced Interleukin-8 (IL-8) Release Is Mediated by S1P Receptor 2 and Nuclear Factor κB in BEAS-2B Cells. PLoS ONE, 9(4), e95566.

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Neural Progenitor Cell Differentiation

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Recombinant human WNT3A (R&D Systems) and SR11302 (TOCRIS) were used at final concentrations of 150 ng/ml and 10 μM, respectively. NPCs of control and APP-KO2 were treated for 48 hours and then stained for SOX2 and DCX.

Shabani K., Pigeon J., Benaissa Touil Zariouh M., Liu T., Saffarian A., Komatsu J., Liu E., Danda N., Becmeur-Lefebvre M., Limame R., Bohl D., Parras C, & Hassan B.A. (2023). The temporal balance between self-renewal and differentiation of human neural stem cells requires the amyloid precursor protein. Science Advances, 9(24), eadd5002.

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Immunoblot Analysis of Cellular Signaling

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[γ-³²P]-ATP was purchased from PerkinElmer (Waltham, MA, USA), bovine serum albumin (BSA) was purchased from Calbiochem (Darmstadt, Germany), and SR-11302 was purchased from Tocris Bioscience (Bristol, United Kingdom). All other reagents were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA).
Antibodies against acetylated-lysine (#9441), total (#9102), and phosphorylated (Thr202-Tyr204, #9101) ERK1/2 MAPK, phosphorylated IκBα (Ser32, #2859), total (#9212) and phosphorylated (Thr180-Tyr182, #9211) p38 MAPK, SIRT3 (#5490) and phosphorylated (Ser727, #9134) STAT3 were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against FOS (sc-253), total IκBα (sc-371), JUN (sc-44), Lamin B (sc-6216), OCT1 (sc-232), p65 (sc-109), and total STAT3 (sc-482) were purchased from Santa Cruz Biotechnology, Inc. (Heidelberg, Germany), acetylated histone 4 (H4) was purchased from R&D Systems (AF5215, Minneapolis, MN, USA), the actin antibody was purchased from Sigma-Aldrich Co. LLC. (A5441), and acetylated-histone 3 (H3, #06-599) and GAPDH antibodies were from Millipore (MAB374, Saint Charles, MO, USA).

Palomer X., Román-Azcona M.S., Pizarro-Delgado J., Planavila A., Villarroya F., Valenzuela-Alcaraz B., Crispi F., Sepúlveda-Martínez Á., Miguel-Escalada I., Ferrer J., Nistal J.F., García R., Davidson M.M., Barroso E, & Vázquez-Carrera M. (2020). SIRT3-mediated inhibition of FOS through histone H3 deacetylation prevents cardiac fibrosis and inflammation. Signal Transduction and Targeted Therapy, 5, 14.

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Western Blot Analysis of Signaling Proteins

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Dulbecco's modified Eagle's medium (DMEM), Roswell Park Memorial Institute (RPMI) 1640, and antibiotics were purchased from Life Technologies (Rockville, USA). Fetal bovine serum (FBS) was purchased from Hyclone (Logan, USA). BBR was purchased from Sigma (St. Louis, USA). SR11302 was purchased from Tocris (Ellisville, USA). The secondary horseradish peroxidase (HRP)-conjugated antibody and mouse monoclonal anti–β-actin antibody were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, USA). Rabbit monoclonal anti-FN antibodies to phospho (p)-c-Jun, total (t)-c-Jun, and c-Fos were purchased from AbCam (Cambridge, UK). The enhanced chemiluminescence (ECL) prime reagents were from Amersham (Buckinghamshire, UK).

Jeong Y., You D., Kang H.G., Yu J., Kim S.W., Nam S.J., Lee J.E, & Kim S. (2018). Berberine Suppresses Fibronectin Expression through Inhibition of c-Jun Phosphorylation in Breast Cancer Cells. Journal of Breast Cancer, 21(1), 21-27.

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Tamoxifen and Estrogen Receptor Signaling

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Tamoxifen (≥99%, ICI146,474, CAS: 10540-29-1) was obtained from MP Biomedicals (Santa Ana, CA). Ethylenediaminetetraacetic acid (≥99%, EDTA, CAS: 60-00-4) and 17β-estradiol (≥98%, E2, CAS: 50-28-1) were obtained from Sigma-Aldrich (St. Louis, MO). Pifithrin α hydrobromide (≥98%, CAS: 63208-82-2), SR 11302 (≥98%, CAS: 160162-42-5), and G-15 (GPER antagonist, >99%, CAS: 1161002-05-6) were obtained from Tocris Bioscience (Minneapolis, MN). GM 6001 (≥98%, CAS: 142880-36-2) was obtained from Enzo Life Sciences (Farmingdale, NY). Dimethyl sulfoxide (≥99.9%, DMSO, CAS: 67-68-5) was obtained from Avantor Performance Materials (Center Valley, PA). All chemicals stocks were prepared in DMSO.

Bugel S.M., Wehmas L.C., La Du J.K, & Tanguay R.L. (2016). Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen’s effects on skin epithelium. Toxicology and applied pharmacology, 296, 31-41.

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