3 trimethylsilyl propionic 2 2 3 3 d4 acid sodium salt tsp d4

Manufactured by Merck Group

Sourced in United States

3-(trimethylsilyl) propionic-2,2,3,3-d4 acid sodium salt (TSP-d4) is a deuterated compound commonly used as a reference standard in nuclear magnetic resonance (NMR) spectroscopy. It provides a stable signal that can be used to calibrate and quantify NMR spectra.

Automatically generated - may contain errors

Lab products found in correlation

Deuterium oxide d2o, by Merck Group (4 mentions) Methanol, by Merck Group (4 mentions) 5 mm tci triple resonance cryoprobe, by Bruker (3 mentions) Topspin software, by Bruker (3 mentions) Hplc grade acetonitrile, by Merck Group (2 mentions) Phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Lysophosphatidylcholine, by Avanti Polar Lipids (1 mentions) Formic acid, by Merck Group (1 mentions) Potassium phosphate dibasic k2hpo4, by Merck Group (1 mentions) Dimethyl sulfoxide d6 dmso d6, by Cambridge Isotopes (1 mentions) Milli q system, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Deuterium oxide, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Solarbio (1 mentions) Advance 500 spectrometer, by Bruker (1 mentions) Methanol d4, by Merck Group (1 mentions) Absolute ethanol, by Tianjin Zhiyuan (1 mentions) Avance 500 spectrometer, by Bruker (1 mentions) Sulforaphane, by LKT Laboratories (1 mentions) Human epidermal growth factor (hegf), by Merck Group (1 mentions)

11 protocols using 3 trimethylsilyl propionic 2 2 3 3 d4 acid sodium salt tsp d4

Quantitative NMR Analysis of Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

All ¹H and ¹³C NMR spectra were recorded at 298 K on a Bruker Avance 500 spectrometer equipped with a 5 mm TCI triple-resonance cryoprobe (PRISM core facility, Rennes). ¹H spectra were acquired with a 6 kHz spectral width, 32 K data points and a total repetition time of 6.73 s. ¹³C spectra were acquired using a proton power-gated decoupling sequence with a 30° flip angle, a 30 kHz spectral width, 64 K data points and a total repetition time of 3.08 s. The data were processed with Topspin software (Bruker Biospin). Before applying the Fourier transform, free induction decays of ¹H spectra were treated with an exponential broadening of 0.3 Hz.
Samples were solubilized in D₂O. 3-(Trimethylsilyl) propionic-2,2,3,3-d4 acid sodium salt (TSP-d4) (Sigma-Aldrich, United States) served as an internal reference for ¹H and ¹³C chemical shifts. The relative concentration of trehalose, glutamate and glycine betaine in the samples was determined by the integration of the peaks’ areas of their ¹H signals relative to the internal standard TMSP. Results are expressed as NMR relative units (RU).

Gaucher F., Kponouglo K., Rabah H., Bonnassie S., Ossemond J., Pottier S., Jardin J., Briard-Bion V., Marchand P., Blanc P., Jeantet R, & Jan G. (2019). Propionibacterium freudenreichii CIRM-BIA 129 Osmoadaptation Coupled to Acid-Adaptation Increases Its Viability During Freeze-Drying. Frontiers in Microbiology, 10, 2324.

+ Open protocol

+ Expand

Astragaloside IV Compound Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Astragaloside IV (purity ≥99%) was obtained from Shanghai Winherb Medical Technology Co. Ltd. (Shanghai, China). Deuterium oxide (D₂O, 99.9% atom %D) and 3-(trimethylsilyl)-propionic-2,2,3,3-d₄ acid sodium salt (TSP-d₄, 98% atom %D) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lyso phosphatidylcholine (12 : 0) and phosphatidylcholine (11 : 0/11 : 0) were purchased from Avanti Polar Lipids (Birmingham, AL, USA).

Jiang M., Ni J., Cao Y., Xing X., Wu Q, & Fan G. (2019). Astragaloside IV Attenuates Myocardial Ischemia-Reperfusion Injury from Oxidative Stress by Regulating Succinate, Lysophospholipid Metabolism, and ROS Scavenging System. Oxidative Medicine and Cellular Longevity, 2019, 9137654.

+ Open protocol

+ Expand

HPLC-Based Ginsenoside Isolation and Characterization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC-grade acetonitrile, methanol, and water were obtained from Merck (Darmstadt, Germany). Formic acid, deuterium oxide (D₂O), and 3-(trimethylsilyl) propionic-2,2,3,3-d₄ acid sodium salt (TSP-d₄) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The ginsenosides were isolated and purified from P. ginseng roots by a series of chromatography procedures in our laboratory, and their structures were elucidated by comparison of spectroscopic data (MS, ¹H-NMR, and ¹³C-NMR) with the literature data: ginsenoside Rb1, Ra2, Ra3, Rc, Ra1, Rb2, Rb3, Rd, F2, Rg3, Rh2, compound K, 20-O-glucoginsenoside Rf, ginsenoside Rg1, Rg2, Re, Rf, Rh1, notoginsenoside R2, ginsenoside F1, Rg6, Rk1, Ro, and Rk3, ginsenoside F4, Rh4, and Rg5 [8 (link)]. The purity of the isolated compounds was determined to be more than 98% by normalization of the peak areas detected by HPLC analysis.

Yoon D., Choi B.R., Kim Y.C., Oh S.M., Kim H.G., Kim J.U., Baek N.I., Kim S, & Lee D.Y. (2019). Comparative Analysis of Panax ginseng Berries from Seven Cultivars Using UPLC-QTOF/MS and NMR-Based Metabolic Profiling. Biomolecules, 9(9), 424.

+ Open protocol

+ Expand

Deuterium Oxide-based NMR Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Deuterium oxide (D₂O, 99% in D) was purchased from Cambridge Isotope Laboratories, Inc., (Andover, MA, USA). Potassium phosphate dibasic (K₂HPO₄, ACS grade reagent), sodium phosphate monobasic (anhydrous), hydrochloric acid (HCl, ACS grade reagent), sodium hydroxide solution (NaOH, HPLC grade), and 3-trimethylsilyl propionic-2,2,3,3-d4 acid sodium salt (TSP-d₄) were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Lee S., Ku J.Y., Kang B.J., Kim K.H., Ha H.K, & Kim S. (2021). A Unique Urinary Metabolic Feature for the Determination of Bladder Cancer, Prostate Cancer, and Renal Cell Carcinoma. Metabolites, 11(9), 591.

+ Open protocol

+ Expand

Scutellarin Tablets and Injection Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Deuterium oxide (D₂O, 99.9%), 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid sodium salt (TSP-d₄, 98% deuterated), methanol, and acetonitrile were purchased from Sigma (Sigma-Aldrich, St. Louis, MO, USA). Dimethyl sulfoxide-d₆ (DMSO-d₆, 99.9% deuterated) was obtained from Cambridge Isotope Laboratories, Inc. (CIL Inc., Andover, MA, USA). DMSO was acquired from Solarbio Technology Co., Ltd (Beijing, China). Ultra-pure water used in the experiments was purified by a Milli-Q system (Millipore, Billerica, MA, USA).
The reference of scutellarin was purchased from Chengdu Pufeide Biotech Co., Ltd (Szechwan, China). The purity of the standard was above 98%.
Tablets were obtained from Yunnan Bio Valley Pharmaceutical Co., Ltd (Yunnan, China), Yunnan Bio Valley Long-kang Pharmaceutical Co., Ltd (Yunnan, China, commissioned production by Yunnan Bio Valley Pharmaceutical Co., Ltd), Yunnan Baiyao Group XingZhong Pharmaceutical Co., Ltd (Yunnan, China), and Yunnan Rainbow Pharmaceutical Group Yuxi Pharmaceutical Co., Ltd (Yunnan, China). Tablets were labeled as T-BV, T-BVLK-1, T-BVLK-2, T-XZ-1, T-XZ-2, T-YX-1, or T-YX-2, respectively. The injectable form was purchased from ShiYao YinHu Pharmaceuticals Co., Ltd (Shanxi, China) and was designated as I-SY.

Jiang Z., Yang J., Jiao Y., Li W., Chai X., Zhang L., Jiang M, & Wang Y. (2016). Determination of scutellarin in breviscapine preparations using quantitative proton nuclear magnetic resonance spectroscopy. Journal of Food and Drug Analysis, 24(2), 392-398.

+ Open protocol

+ Expand

NMR Characterization of Metabolic Profiles

Check if the same lab product or an alternative is used in the 5 most similar protocols

All ¹H and ¹³C NMR spectra were recorded at 298 K on a Bruker Advance 500 spectrometer equipped with a 5 mm TCI triple-resonance cryoprobe (PRISM core facility, Rennes, France). ¹H spectra were acquired with a 6 kHz spectral width, 32 K data points and a total repetition time of 6.73 s. ¹³C spectra were acquired using a proton power-gated decoupling sequence with a 30° flip angle, a 30 kHz spectral width, 64 K data points and a total repetition time of 3.08 s. The data were processed with Topspin software (Bruker Biospin, Wissembourg, France). Before applying the Fourier transform, free induction decays of ¹H spectra were treated with an exponential broadening of 0.3 Hz.
Samples were solubilized in D₂O. 3-(trimethylsilyl) propionic-2,2,3,3-d4 acid sodium salt (TSP-d4) (Sigma-Aldrich, Saint Louis, MO, USA) served as an internal reference for ¹H and ¹³C chemical shifts.
The relative concentration of trehalose, glutamate, proline, and glycine betaine in the samples was determined by integration of their ¹H signals. Results are expressed as NMR relative units (RU) reported to one unit of optical density (equivalent to 1 mL of culture at an OD of 1).

Gaucher F., Gagnaire V., Rabah H., Maillard M.B., Bonnassie S., Pottier S., Marchand P., Jan G., Blanc P, & Jeantet R. (2019). Taking Advantage of Bacterial Adaptation in Order to Optimize Industrial Production of Dry Propionibacterium freudenreichii. Microorganisms, 7(10), 477.

+ Open protocol

+ Expand

Analytical Characterization of Chinese Herbal Medicine

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fructose (purity ≥ 99.8%), D-glucose anhydrous (purity ≥ 99.9%), sucrose (purity ≥ 99.8%) and maltose (purity ≥ 99.8%) were purchased from National Institutes for Food and Drug Control (Beijing, China), and their chemical structures were shown in Figure 1. 1-Phenyl-3-methyl-5-pyrazolone (PMP, analytical grade and purity ≥ 99.5%) was obtained from Tianjin kemio Chemical Reagent Co., Ltd. (Tianjin, China). Absolute ethanol (analytical grade) was purchased from Tianjin Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China). HPLC-grade acetonitrile and methanol were purchased from Merck (Darmstadt, Germany). Deuterium oxide (D₂O, 99.9 atom % D), methanol-d₄ (CD₃OD, 99.8 atom % D), and 3-(Trimethylsilyl)propionic-2,2,3,3-d₄ acid sodium salt (TSP-d₄, 98 atom % D) were purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). YQFM samples of 10 batches (20190409, 20190514, 20190707, 20190906, 20191101, 20201005, 20200809, 20201105, 20210810 and 20210909) were obtained from Tianjin Tasly Pride Pharmaceutical Co., Ltd. (Tianjin, China), numbered S1~S10.

Xie Y., Zheng D., Yang T., Zhang Z., Xu W., Liu H, & Li W. (2023). Head-to-Head Comparison of High-Performance Liquid Chromatography versus Nuclear Magnetic Resonance for the Quantitative Analysis of Carbohydrates in Yiqi Fumai Lyophilized Injection. Molecules, 28(2), 765.

+ Open protocol

+ Expand

NMR Analysis of Bacterial Osmoprotectants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Osmoprotectants were then identified and quantified by nuclear magnetic resonance (NMR) as previously described for other bacteria. All ¹H and ¹³C NMR spectra were recorded at 298 K on a Bruker Avance 500 spectrometer equipped with a 5-mm TCI triple-resonance cryoprobe (PRISM Core Facility, Rennes). ¹H spectra were acquired with a 6-kHz spectral width, 32 K data points, and a total repetition time of 6.73 s. ¹³C spectra were acquired using a proton power-gated decoupling sequence with a 30° flip angle, a 30-kHz spectral width, 64 K data points, and a total repetition time of 3.08 s. The data were processed with Topspin software (Bruker Biospin). Before applying the Fourier transform, free induction decays of ¹H spectra were treated with an exponential broadening of 0.3 Hz.
Samples were solubilized in D₂O (Sigma-Aldrich, USA). 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP-d4) (Sigma-Aldrich, USA) served as an internal reference for ¹H and ¹³C chemical shifts. The relative concentration of trehalose, glutamate, and glycine betaine in the samples was determined by the integration of the peaks areas of their ¹H signals relative to the internal standard TMSP.

Gaucher F., Rabah H., Kponouglo K., Bonnassie S., Pottier S., Dolivet A., Marchand P., Jeantet R., Blanc P, & Jan G. (2020). Intracellular osmoprotectant concentrations determine Propionibacterium freudenreichii survival during drying. Applied Microbiology and Biotechnology, 104(7), 3145-3156.

+ Open protocol

+ Expand

Sulforaphane and TSP-d4 Procurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sulforaphane was purchased from LKT Laboratories, Inc. (St. Paul, MN, USA) and 3-(trimethylsilyl) propionic-2,2,3,3-d4 acid sodium salt (TSP-d₄) were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Kim H., Yoo S., Lee J.D., Kim H.Y., Kim S, & Kim K.B. (2022). A Metabolomics Approach to Sulforaphane Efficacy in Secondhand Smoking-Induced Pulmonary Damage in Mice. Metabolites, 12(6), 518.

+ Open protocol

+ Expand

NMR Analysis of Cheese Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

3-(trimethylsilyl) propionic-2,2,3,3-d4 acid sodium salt (TSP-d4) and deuterium oxide (D2O) were obtained from Sigma-Aldrich Co (St. Louis, MO, USA). For NMR analysis, each cheese sample weighing 20 mg added into a 4 mm NMR nanotube followed by addition of 20 μL of 2 mM TSP-d4 solution in D₂O.

Kandasamy S., Yoo J., Yun J., Kang H.B., Seol K.H, & Ham J.S. (2020). 1H HRMAS-NMR based metabolic fingerprints for discrimination of cheeses based on sensory qualities. Saudi Journal of Biological Sciences, 27(6), 1446-1461.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!