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Sirna against luciferase

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The SiRNA against luciferase is a laboratory reagent used for the knockdown of luciferase gene expression. It is a small interfering RNA (siRNA) designed to target and suppress the luciferase gene, which is commonly used as a reporter gene in various experimental systems.

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Lab products found in correlation

Hiperfect transfection reagent, by Qiagen (1 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Luciferase siRNA SiRNAs

3 protocols using sirna against luciferase

1

Top2β Downregulation in Neurospheres

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Top2β was downregulated using SmartPOOL RNA mix of 4 siRNAs (Dharmacon, CatN: L-004240-00) and HiPerfect transfection reagent (Qiagen). NCH421k neurospheres or primary GSC spheres were broken to single cell suspension before transfection. Optimal downregulation was achieved by double transfection with 50 nM siRNA and was stable between 48 and 120 h after transfection. RNAi control experiments were performed using siRNA against Luciferase (Dharmacon).
Kenig S., Faoro V., Bourkoula E., Podergajs N., Ius T., Vindigni M., Skrap M., Lah T., Cesselli D., Storici P, & Vindigni A. (2016). Topoisomerase IIβ mediates the resistance of glioblastoma stem cells to replication stress-inducing drugs. Cancer Cell International, 16, 58.
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2

Silencing of human MAD2 in HEK-293 cells

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HEK‐293 cells in different culture formats were transfected with three target‐specific siRNAs against human MAD2 (sc‐35837, Santa Cruz Biotechnology) or with siRNA against luciferase (Dharmacon) as a control. Lipofectamine RNAiMax transfection reagent (13778150, Invitrogen) was used for complex formation at a ratio of 3 pmol siRNA/μl. The final siRNA concentration in the culture medium was 10 nM. Gene silencing was allowed to proceed for 48 h.
Koban F, & Freissmuth M. (2023). The cell cycle protein MAD2 facilitates endocytosis of the serotonin transporter in the neuronal soma. EMBO Reports, 24(10), e53408.
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3

Transient siRNA Knockdown in H1299 Cells

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For transient small interfering RNA (siRNA) experiments, H1299 cells were seeded in 6-cm2 dishes and transfected using Lipofectamine RNAiMax (Invitrogen, Carlsbad, CA, USA) with siRNA against luciferase (Dharmacon, Lafayette, CO, USA) as a control and siRNA against E6AP (Dharmacon).
Tomaić V, & Banks L. (2015). Angelman syndrome-associated ubiquitin ligase UBE3A/E6AP mutants interfere with the proteolytic activity of the proteasome. Cell Death & Disease, 6(1), e1625-.
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