Anti p erk antibody

Unless specified, all reagents were obtained from Sigma. His-tagged recombinant ERMP1 domains were generated in E. coli as described [10 (link)]. Human cells were obtained from the ATCC collection and, unless differently stated, cultured under ATCC recommended conditions. Anti-HIF-1 antibody was from BD Transduction Laboratories. Anti-Nrf2, anti-actin and anti-p-PERK antibodies were from Santa Cruz Biotechnology. Anti-PERK antibody was from Cell Signaling Technology. The four TMAs used were produced within previous Biobank activities at Pathology of the University Hospital of Basel. All activities were conducted previous acquirement of the local ethical committee permission. A commercial TMA (MNO961, Pantomics, Richmond, CA, USA) carrying normal samples from 33 organs was also used to assess ERMP1 staining.

AVM (containing 93% avermectin B1a and 7% avermectin B1b) was obtained from the ZND Bio-technology Co., Ltd. (Beijing, China), and the anti-AVM antibody was a gift from Professor Shen in CAU (Beijing, China). The procedures for preparing the anti-AVM antibody were as follows. First, the structure of AVM was modified by succinylation. Then, the 4″-O-succinyl-AVM was conjugated to bovine serum albumin (BSA). This immunizing antigen was then injected into rabbits, and the polyclonal antibody was acquired [56 (link)].
Lapatinib (EGFR phosphorylation inhibitor) was purchased from Selleck (Houston, TX). Wortmanin (phosphoinositide-3-kinase (PI3K) inhibitor) and U0126 (mitogen-activated protein kinase kinase (MEK) inhibitor) were purchased from Kinasechem (UK). The Relish inhibitor pyrrolidinedithiocarbamic acid (PDTC) was purchased from Sigma-Aldrich (St Louis, MO). The mouse monoclonal anti-P-gp antibody (C219) was purchased from Calbiochem (Darmstadt, Germany). Anti-p-AKT antibody, anti-p-ERK antibody, anti-AKT antibody and anti-ERK antibody were purchased from Cell Signaling Technology (Boston, MA). Anti-EGFR antibody was purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-p-Tyr antibody was purchased from BD Biosciences (Franklin Lakes, NJ). Anti-Relish antibody was purchased from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA).