Collagen 1 from rat tail

Manufactured by BD

Sourced in United States

Collagen-I from rat tail is a natural extracellular matrix protein extracted from the tails of rats. It serves as a structural component for various cell and tissue culture applications.

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Lab products found in correlation

Hcpl fluotar 20 objective, by Leica (1 mentions) Alkaline phosphatase conjugated anti rabbit antibody, by Merck Group (1 mentions) Collagen 4 from bovine placentavilli, by Merck Group (1 mentions) Celltracker green 5 chloromethylfluorescein diacetate cmfda, by Thermo Fisher Scientific (1 mentions) P nitrophenyl phosphate, by Merck Group (1 mentions) 96 well polystyrene plate, by Thermo Fisher Scientific (1 mentions) Rpmi medium, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions) Il 1β, by Miltenyi Biotec (1 mentions) Tnf α, by R&D Systems (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Jnk inhibitor sp600125, by Selleck Chemicals (1 mentions) Tissue culture flask, by Corning (1 mentions) Egm 2, by Lonza (1 mentions)

Spelling variants of this product

Collagen I (161 citations) Rat tail collagen I (107 citations) Rat tail collagen (72 citations) Type I collagen from rat tail (20 citations) Type 1 collagen from rat tail tendon (6 citations) Rat collagen I (3 citations)

6 protocols using collagen 1 from rat tail

Fibrinogen and Thrombin Protocol

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Fibrinogen from bovine plasma Type I-S, 65–85% protein (≥75% of protein is clottable) and thrombin from bovine plasma lyophilized powder, 40–300 NIH units/mg protein were purchased from Sigma Aldrich (St. Louis, MO). Collagen I from rat-tail was purchased from BD Biosciences (San Jose, CA). All other reagents were of chemical grade.

Albanna M., Binder K.W., Murphy S.V., Kim J., Qasem S.A., Zhao W., Tan J., El-Amin I.B., Dice D.D., Marco J., Green J., Xu T., Skardal A., Holmes J.H., Jackson J.D., Atala A, & Yoo J.J. (2019). In Situ Bioprinting of Autologous Skin Cells Accelerates Wound Healing of Extensive Excisional Full-Thickness Wounds. Scientific Reports, 9, 1856.

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Boyden's Chamber Assay for Cell Migration

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Boyden’s chamber assay was performed using collagen-I from rat-tail (BD Biosciences)-coated Transwells with 8 μm polycarbonate membrane (Corning) [38 (link)]. Cycling cells were plated in the upper chamber at 5 × 10⁴ per well in 150 µL complete medium.
When indicated, MCF-7 conditioned medium was added to the lower chambers. Cells were allowed to migrate for 36 h in a humidified incubator at 37 °C with 5% CO₂ in the absence or presence of the compound added to the upper and lower chambers at the indicated concentrations.
After 36 h, non-migrating cells from the membrane upper surface were removed using a sterile cotton swab. The polycarbonate membranes were fixed for 20 min in 4% paraformaldehyde, stained with Hoechst, removed with forceps from the companion chamber and mounted. Migrating cells from at least 30 fields/each membrane were counted as described [38 (link)], using a DMBL fluorescent microscope (Leica), equipped with an HCPL Fluotar 20× objective. Data are representative of three different experiments.

Carafa V., Poziello A., Della Torre L., Giovannelli P., Di Donato M., Safadeh E., Yu Z., Baldi A., Castoria G., Tomaselli D., Mai A., Rotili D., Nebbioso A, & Altucci L. (2019). Enzymatic and Biological Characterization of Novel Sirtuin Modulators against Cancer. International Journal of Molecular Sciences, 20(22), 5654.

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Collagen Gel Synthesis and Polymerization

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Formulation and synthesis of collagen gels were performed using a protocol described elsewhere.^{26 (link)}Briefly, collagen gels were synthesized using high concentration collagen-I from rat tail (BD Biosciences, San Jose, CA). Collagen-I was diluted to two final concentrations of 2 and 4 mg/mL as follows. Equal volume of collagen-I and 100 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer solution in 2X phosphate buffered saline, PBS (pH 7.3) were mixed to reach the final concentration. Gel solution was then placed on a 35 mm glass bottom petri dish (In vitro scientific, Sunnyvale, CA) and allowed to polymerize completely for 90 mins at 37°C and 5% CO₂. Consequently, cells were seeded on polymerized gels and were incubated at 37°C and 5% CO₂. The reported shear modulus values were ~104 and 391 Pa corresponding to final collagen concentrations of 2 and 4 mg/ml in precursor solution.^{26 (link)}

Ali M.Y., Chuang C.Y, & Saif M.T. (2014). Reprogramming Cellular Phenotype by Soft Collagen Gels. Soft matter, 10(44), 8829-8837.

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Collagen-Based Cell Culturing Protocol

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Collagen IV (from bovine placenta villi) was purchased from Chemicon (Temecula, CA, USA) and collagen I (from rat tail) from BD Biosciences (Bedford, MA, USA). The 96-well polystyrene plates were obtained from Nunc (Roskilde, Denmark). Bovine serum albumin (BSA), Hank’s balanced salt solution (HBSS) sulfate, alkaline phosphatase-conjugated anti-rabbit antibody, and p-nitrophenyl phosphate were purchased from Sigma-Aldrich (St Louis, MO, USA). CellTracker™ green 5-chloromethylfluorescein diacetate (CMFDA) and rabbit polyclonal antibodies against glutathione s-transferase (GST) were purchased from Molecular Probes (Eugene, OR, USA).

Momic T., Katzhendler J., Shai E., Noy E., Senderowitz H., Eble J.A., Marcinkiewicz C., Varon D, & Lazarovici P. (2015). Vipegitide: a folded peptidomimetic partial antagonist of α2β1 integrin with antiplatelet aggregation activity. Drug Design, Development and Therapy, 9, 291-304.

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Modulating Aortic Endothelial Cell Signaling

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Human aortic endothelial cells (HAoECs) were purchased from LifeLine Cell Technologies and maintained in VascuLife VEGF media (LifeLine Cell Technolgies). Cells between passages 3 and 11 were used for experiments. HAoECs were pretreated for 1 h with either the FAK/Pyk2 inhibitor PF-271 (MedKoo), the FAK inhibitor PF-228 (MedKoo), the FAK/Pyk2 inhibitor PF-271 VS-6063 (MedChemExpress), the Src inhibitor Dasatinib (Selleckchem), the MEK inhibitor PD98059 (Tocris), or the JNK inhibitor SP600125 (Selleckchem) prior to stimulation with either TNF-α (R&D) or IL-1β (Miltenyi Biotec). THP-1 cells were purchased from ATCC and maintained in RPMI Medium (Sigma-Aldrich) supplemented with 10% FBS (Omega Scientific). Collagen I from rat tail was purchased from BD Biosciences.

Murphy J.M., Jeong K., Rodriguez Y.A., Kim J.H., Ahn E.Y, & Lim S.T. (2019). FAK and Pyk2 activity promote TNF-α and IL-1β-mediated pro-inflammatory gene expression and vascular inflammation. Scientific Reports, 9, 7617.

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Endothelial Cell Culture Protocol

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After isolation, cell cultures were maintained in endothelial growth medium (EGM-2, Lonza) supplemented with 8% fetal bovine serum for a total of 10%. Unless otherwise specified, all cell expansions occurred in tissue culture flasks (Corning) pre-coated with collagen-I from rat tail (BD Biosciences). All cell culture experiments used EGM-2 supplemented with 8% FBS. During expansions and experiments, cells were kept in a sterile, humidified incubator at 37°C and 5% CO₂.

Hagen M.W, & Hinds M.T. (2019). Static spatial growth restriction micropatterning of endothelial colony forming cells influences their morphology and gene expression. PLoS ONE, 14(6), e0218197.

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