Myricetin 3 o galactoside

Acetonitrile was HPLC grade and was purchased from Sigma (Merck Life Science S.r.l., Milano, Italy). Orthophosphoric acid (ACS ISO, for analysis, 85%) and ethanol (ACS-Reag. Ph.Eur. Supelco^®, Milan, Italy) were purchased from Carlo Erba..Water was distilled and filtered through a Milli-Q apparatus (Millipore, Milan, Italy).
Standards of gallic acid and ellagic acid were purchased from Sigma (Merk Life Science S.r.l., Milan, Italy). Standards of myricetin-3-O-galactoside, myricetin-3-O-rhamnoside, quercetin-3-O-glucoside, quercetin-3-O-rhamnoside, quercetin 3-O-galactoside, vitexin, cyanidin 3-O-glucoside, petunidin 3-O-glucoside, peonidin 3-O-glucoside and malvidin 3-O-glucoside were purchased from Extrasynthese (Lyon, France).

Commercial extracts of RO and BC were obtained from PK Components (Warsaw, Poland). Cyanidin-3-O-glucoside, a 3-O-rutinoside, delphinidin-3-O-glucoside, delphinidin-3-O-rutinoside, quercetin-3-O-glucoside, quercetin-3-O-rutinoside, myricetin-3-O-rutinoside, myricetin-3-O-galactoside, kaempferol-3-O-rutinoside, (+)-catechin, and (−)-epicatechin were purchased from Extrasynthese (Lyon, France). Neochlorogenic acid was obtained from TRANS MIT GmbH (Giessen, Germany). Acetonitrile was purchased from Merck (Darmstadt, Germany). The extracts were preliminarily tested for 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH) to assess the synthetic capacity to neutralize the reactive oxygen species that are the main mediator of peroxidation process [25 ,26 (link)].

Gallic acid, caffeic acid, ellagic acid, quercetin, flavone, catechin, epicatechin, epigallocatechin, epicatechin gallate, epigallocatechin gallate, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and the Folin-Ciocalteu reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). quercetin-3-galactoside, quercetin-3-rhamnoside, myricetin-3-O-galactoside, myricetin-3-O-glucoside, procyanidin B1 and procyanidin B2 were supplied by Extrasynthese (Genay, France). All chemicals and reagents were of analytical grade unless specified.