Transmission electron microscopy (TEM) images of micelles were acquired on a Japan Hitachi HT7700 electronic microscope by negatively staining the micelles with 2.0% phosphotungstic acid. The hydrodynamic diameter and zeta potential of micelles were determined using a dynamical light scattering (DLS) system (NanoBook Omni Instrument, United States). UV-vis spectra were recorded with a UV-2450 UV-vis spectrometer (Shimadzu, Japan). Fluorescent images were captured on a Leica TCS SP8 confocal laser scanning microscopy (CLSM) (Leica, Germany). Cell apoptosis was measured by a BD FACS Melody flow cytometer.
Uv 2450 uv vis spectrometer
Manufactured by Shimadzu
Sourced in Japan
The UV-2450 UV-Vis spectrometer is a laboratory instrument designed to measure the absorbance or transmittance of a sample across a range of ultraviolet and visible light wavelengths. It is capable of performing spectroscopic analysis to determine the concentration and identity of chemical compounds in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Facsmelody flow cytometer, by BD (1 mentions)
Ltq orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Rf 5301pc luminescence spectrometer, by Shimadzu (1 mentions)
Nicolet is10 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions)
Amicon ultra 0.5 ml centrifugal filter, by Merck Group (1 mentions)
6 protocols using uv 2450 uv vis spectrometer
1
Characterization of Micelle Nanostructure and Cellular Apoptosis
2
NMR and Mass Spectroscopy Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemicals for synthesis were purchased from commercial suppliers and were used as received without further purification. 1H and 13C NMR spectra were measured in CDCl3 or DMSO-d6 with a Bruker AV spectrometer operating at 400 MHz and 100 MHz, respectively and chemical shifts were reported in ppm using tetramethylsilane (TMS) as the internal standard. Mass spectra were obtained with a Thermo LTQ Orbitrap mass spectrometer. UV-vis absorption and fluorescence emission spectra were recorded with a Shimadzu UV-2450 UV/vis spectrometer and a Shimadzu RF-5301pc luminescence spectrometer, respectively.
3
UV-Vis Absorption Spectroscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Absorption spectra were obtained with a UV-2450 UV-Vis spectrometer (Shimadzu, Japan) at 288 K in the range of 200–500 nm using a 1 cm quartz cell. The method employed was the same as that described in Section 2.2.1 .
4
Optical Characterization of h-EAO Phosphor
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The optical properties of the h-EAO phosphor were studied using an array of spectral methods. The PL spectra were recorded using a FluoroLog-3 spectrofluorometer equipped with a 450-W xenon arc lamp as the excitation source. The diffuse reflectance absorption was measured using a Shimadzu UV-2450 UV-Vis spectrometer. The luminescence lifetime was recorded using a photomultiplier tube and a digital oscilloscope under excitation with the third harmonic (355 nm) of a Nd:YAG pulsed laser. The TL curves were recorded using the Fluorolog-3 spectrofluorometer and a homemade TL setup. Appropriate optical filters were used to avoid stray light in all optical measurements.
5
Spectroscopic Analysis of PSP and PSP-Zn
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The FT-IR of PSP and PSP−Zn were obtained on a Nicolet iS10 FT-IR spectrometer (Thermo Fisher Scientific co., Ltd., Waltham, MA, USA) over the range of 400–4000 cm−1 with 32 scanning times.
The UV-Vis spectra of the PSP and PSP−Zn solutions (2 mg/mL) were obtained on a UV-2450 UV-Vis spectrometer (Shimadzu, Japan) over the wavelength range of 200–500 nm.
The UV-Vis spectra of the PSP and PSP−Zn solutions (2 mg/mL) were obtained on a UV-2450 UV-Vis spectrometer (Shimadzu, Japan) over the wavelength range of 200–500 nm.
6
ABDM Crosslinked F1 ATPase Spectroscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The absorption spectra shown in Figs. S1 and S2 were recorded with a Shimadzu UV-2450 UV-VIS spectrometer. The samples were prepared by washing and concentrating ABDM crosslinked F1 αA380C/βV409C in buffer A using an Amicon-ultra 0.5 ml centrifugal filter with 50 kDa pore size (Millipore, Billerica, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!