Palmitic acid d4

Manufactured by Cambridge Isotopes

Palmitic acid (D4) is a stable isotopically labeled compound used as a research tool. It is a deuterium-labeled form of the common saturated fatty acid palmitic acid. Palmitic acid (D4) can be utilized in various analytical and experimental applications that require a labeled fatty acid compound.

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Lab products found in correlation

Milli q, by Merck Group (1 mentions) High performance liquid chromatography (hplc), by Thermo Fisher Scientific (1 mentions) Phenylalanine d 5, by Cambridge Isotopes (1 mentions)

2 protocols using palmitic acid d4

Plasma/Serum Metabolite Extraction for LC-MS

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All solvents used below were of HPLC grade (Fisher Chemicals) and the water was Milli-Q (Millipore).
Plasma and serum samples were thawed on ice and 50 µl aliquots were mixed with 150 µl MeOH for protein precipitation. Samples were then centrifuged for 15 min at 15800×g. The supernatant was transferred to a new tube and then evaporated to complete dryness in a vacuum concentrator. Prior to LC-MS analysis, samples were reconstituted in 50 µl MeOH:H₂O 1:1 and the two isotopically labelled internal standards phenylalanine (D5) and palmitic acid (D4) (Cambridge Isotope Laboratories) were added at a final concentration of 5 µM.

Lindahl A., Heuchel R., Forshed J., Lehtiö J., Löhr M, & Nordström A. (2017). Discrimination of pancreatic cancer and pancreatitis by LC-MS metabolomics. Metabolomics, 13(5), 61.

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Synthetic Lipid Standards Acquisition

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All synthetic phospholipids (di14:1 phosphatidylcholine (PC), di16:1 phosphatidylethanolamine (PE), di14:0 phosphatidic acid (PA), di 14:0 phosphatidylserine (PS) and di15:0 phosphatidylglycerol (PG)), lysophospholipid (17:0 LPC, 14:0 LPE) species, N-dodecanoyl sphingomyelin (N12:0 SM), and N-heptadecanoyl ceramide (N17:0 Cer) used as internal standards were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). Triheptadecanoylglycerol (T17:1 TAG) and 1,3-dipentadecanoin (1,3-D15:0 DAG) were purchased from Nu Chek, Inc. (Elysian, MN). Palmitic acid-d4 was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). All of the solvents were purchased from Burdick and Jackson (Honeywell International Inc., Muskegon, MI). All other chemical reagents of at least analytical grade or the best grade available were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO), Fisher Scientific (Pittsburgh, PA), or as indicated.

Hayakawa J., Wang M., Wang C., Han R.H., Jiang Z.Y, & Han X. (2017). Lipidomic analysis reveals significant lipogenesis and accumulation of lipotoxic components in ob/ ob mouse organs. Prostaglandins, leukotrienes, and essential fatty acids, 136, 161-169.

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