Zorbax sb c18 4.6 250 mm column

Manufactured by Agilent Technologies

Sourced in United States

The Zorbax SB-C18 4.6 × 250 mm column is a reversed-phase high-performance liquid chromatography (HPLC) column. It features a silica-based stationary phase with a C18 bonded ligand. The column dimensions are 4.6 millimeters in diameter and 250 millimeters in length.

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Lab products found in correlation

1260 infinity high performance liquid chromatograph, by Agilent Technologies (1 mentions) Accq fluor reagent, by Waters Corporation (1 mentions)

Close spelling variants of this product

ZORBAX SB-C18 4.6 × 250 mm Zorbax SB-C18 column Zorbax SB-C18 Zorbax 250 column Zorbax C18 column SB-C18 column C18 ZORBAX SB-C18 C18 column

2 protocols using zorbax sb c18 4.6 250 mm column

Polyphenol Analysis in Edible Packaging

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HPLC chromatograph, 1260 Infinity high performance liquid chromatograph (Agilent Technologies, Santa Clara, CA, USA) was used to determine polyphenolic compounds in the experimentally produced edible packaging. The method of Gómez-Estac et al. [27 (link)] with slight modifications was used. The mobile phase consisted of 1% phosphoric acid (A) and acetonitrile (B) in the following composition: 80% of A and 20% of B for 20th min, 70% of A and 30% of B from 20th to 25th min, 60% of A and 40% of B from 25th to 40th min. The separation was performed on a Zorbax SB-C18 4.6 × 250 mm column (Agilent Technologies, Santa Clara, CA, USA) (the temperature was 25 °C) and detection was performed on a DAD array detector (detection wavelength was 324.5 nm) The injection volume was 10 µL. Each sample was measured in triplicate. The software used for HPLC chromatohraph was Agilent ChemStation.

Dordevic S., Dordevic D., Sedlacek P., Kalina M., Tesikova K., Antonic B., Tremlova B., Treml J., Nejezchlebova M., Vapenka L., Rajchl A, & Bulakova M. (2021). Incorporation of Natural Blueberry, Red Grapes and Parsley Extract By-Products into the Production of Chitosan Edible Films. Polymers, 13(19), 3388.

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HPLC Analysis of Tea Compounds

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A Water Alliance 2695 HPLC system coupled with a photodiode array detector and Empower 2 software was used to analyze the catechins, gallic acid, theaflavins, and thearubigin fraction and caffeine. The separation of compounds was conducted on an Agilent Zorbax SBC18 4.6 × 250 mm column (Agilent) with a gradient of acetonitrile (solution A) and 0.1% phosphoric acid in water (solution B). The gradient started at 5% solution A and 95% solution B, increasing over 40 min to 40% solution A and 60% solution B. The detection wavelength was 280 nm for catechins, 440 nm for theaflavin fraction, and 400 nm for thearubigin fraction. Theanine was determined after reaction with AccQ Fluor reagent (Waters WAT-052880) and measured by Waters 474 fluorescence detector.

Henning S.M., Yang J., Hsu M., Lee R.P., Grojean E.M., Ly A., Tseng C.H., Heber D, & Li Z. (2017). Decaffeinated green and black tea polyphenols decrease weight gain and alter microbiome populations and function in diet-induced obese mice. European journal of nutrition, 57(8), 2759-2769.

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