Cells were treated with PBS or 10 nM Dd-AF647 for 2 h at 37°C. After incubation, cells were fixed with 1× FACS lysing solution (BD Biosciences). Nuclei were stained with DAPI (1:3,000; Sigma) and membranes with Alexa Fluor 555-conjugated wheat germ agglutinin (WGA; 1:200; Invitrogen) during 10 min at room temperature. Images were acquired after sequential laser excitation using an LSM 710 NLO laser-scanning microscope (Zeiss) onto optical sectioning of 1 μm and analyzed with ImageJ software.
Lsm 710 nlo laser scanning microscope
Manufactured by Zeiss
Sourced in Germany
The LSM 710 NLO is a laser scanning microscope designed for advanced imaging applications. It features a near-infrared laser for non-linear optical (NLO) excitation, enabling high-resolution imaging of thick samples and live specimens. The microscope provides a versatile platform for a range of imaging techniques, including multiphoton fluorescence, second harmonic generation, and coherent anti-Stokes Raman scattering (CARS).
Automatically generated - may contain errors
3 protocols using lsm 710 nlo laser scanning microscope
1
Visualizing Cell Membrane and Nucleus
2
Transformation of Arabidopsis with pRI101-35S:TaLAMP1-3B–eGFP
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pRI101-35S:TaLAMP1-3B–eGFP was transformed into Arabidopsis by dipping method; root tip cells of T2 lines were observed with a Zeiss LSM 710 NLO laser scanning microscope (Zeiss, Germany)1 with 488- and 543-nm laser.
3
Fluorescent Protein Fusion Imaging
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The ORF of SiMYB3 was cloned into the p16318hGFP vector and fused with the GFP reporter gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The protoplast transformation was performed using the method described by Asai et al. [59 (link)]. The protoplasts were then viewed with a Zeiss LSM 710 NLO laser scanning microscope (Zeiss, Oberkochen, Germany, http://corporate.zeiss.com ) with a 488- or 543-nm laser.
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