E0771

Medullary tumor cell line E0771 (RRID: CVCL_GR23), melanoma cell line B16 (CVCL_0157), and cervical cancer cell line U14 (CVCL_9U56) with C57/BL6J genetic background were from ATCC. E0771 and B16/U14 cells were maintained in RPMI 1640 and DMEM supplemented with 10% fetal bovine serum (Gibco) at 37 °C, 100% humidity, and 5% CO₂. Human breast cancer cell lines T47D (CVCL_0553), MCF7 (CVCL_0031), ZR-75-30 (CVCL_1661), and MDA-MB-231 (CVCL_0062) were gifted from Jilin People’s Hospital and cultured as described^{52 (link)}.

Mouse BC cells 4T1 have been maintained in our laboratory and mouse BC cells E0771 were purchased from CH3 Biosystems (#94A001, Amherst, NY). The 4T1 and E0771 cells and human BC cells MCF-7 and MDA-MB-231 were cultured in DMEM (Gibco, Carlsbad, CA) with 10% FBS, 100 U/ml penicillin, and 100 mg/ml streptomycin. All cell lines were analyzed and went through authentication by targeted genomic and RNA sequencing.

Normal hepatocytes, AML12 (alpha mouse liver 12), and MIHA (Immortalized human hepatocytes), human breast cancer cell lines (MCF-7 cells), and mouse mammary carcinoma cell lines (T47D, E0771, and 4T1) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA; passage number: 5–15) and maintained according to their instructions. The MIHA and E0771 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, 1 g/L glucose) and Ham’s F12 1:1 medium with 0.005 mg/mL insulin, 0.005 mg/mL transferrin, 5 ng/mL selenium, and 40 ng/mL dexamethasone (Gibco, Grand Island, NY, USA). AML12 cells were grown in DMEM-F12; MCF-7 cells were cultivated in Eagle’s Minimum Essential Medium (Gibco, Grand Island, NY, USA); 4T1 and T47D cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco, Grand Island, NY, USA); and all cells were seeded to reach about 80% confluence prior to experimental treatment. All these cells were supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA), streptomycin (100 μg/mL), and penicillin-streptomycin (FBS, 100 U/mL, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C in a humidified atmosphere of 95% air, 5% CO₂.

E0771 and PY8119 Mus musculus mammary gland adenocarcinoma cell lines were acquired from American Type Cell Culture (ATCC, Manassas, VA, USA) and stored according to the supplier’s instructions. E0771 cells were maintained in Dulbecco’s Modified Eagle Medium (GIBCO, Thermo Fisher Scientific, Waltham, MA, USA) with 25 mM glucose, 4 mM glutamine, and 25 mM HEPES, supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS) (Thermo Fisher Scientific, Waltham, MA, USA). PY8119 cells were maintained in culture in F-12K medium (GIBCO, Thermo Fisher Scientific, Waltham, MA, USA) with 7 mM glucose and 2 mM glutamine, supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific, Waltham, MA, USA). They were cultured in a humidified atmosphere at 37 °C and 5% CO₂.

4T1 (ATCC, CRL- 2539) and E0771 (CH3 Biosystems, 94001) cell lines were kindly donated by Dra. Miriam Lopes and Dra. Catarina Raposo Dias Carneiro from the Institute of Biological Sciences at the Federal University of Minas Gerais/MG, and the Faculty of Pharmaceutical Sciences at the. State University of Campinas/SP, respectively. 4T1 and E0771 cell lines were cultured in RPMI-1640 medium (Gibco) supplemented with 10% Fetal Bovine Serum and 1% Streptomycin/Penicillin (Gibco), and kept at 37 °C and 5% CO₂ incubator. The cell culture medium was replenished every two or three days, and cell lines were subcultured when cells achieved 70% confluence.