Acs grade solvents

Manufactured by Thermo Fisher Scientific

Sourced in United States

ACS grade solvents are high-purity organic solvents that meet the standards set by the American Chemical Society (ACS) for chemical reagents. These solvents are commonly used in analytical and research applications where consistency and reliability are critical.

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ACS grade (14 citations)

6 protocols using acs grade solvents

Colorectal Cancer Cell Antioxidant Assays

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ACS-grade solvents (acetone, methanol, and 95% ethanol), HPLC-grade solvents (water, methanol, and acetonitrile), glass wool, Gibco fetal bovine serum, glacial acetic acid and hydrochloric acid were acquired from the Fisher Scientific Co., LLC (Suwanee, GA, USA). Sephadex LH-20, Amberlite XAD-16, Folin & Ciocalteu’s phenol reagent, gallic acid (>99%), (+)-catechin hydrate (>99%), chlorogenic acid (≥98%), protocatechuic acid (≥97%), vanillic acid (≥97%), fluorescein 3′,6′-dihydroxyspiro[isobenzofuran-1[3H]9′[9[H]xanthen]-3-one), Trolox [(±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid, 97%], TPTZ (2,4,6-tripyridyl-S-triazine, ≥99.0%), quercetin-3-O-rutinoside hydrate (≥94%), iron(II) sulfate heptahydrate (≥99.0%), iron(III) chloride hexahydrate (97%), 2′,7′-dichlorofluorescin diacetate (DCFH-DA, ≥97%), and 2,2′-azobis [2-amidinopropane] dihydrochloride (ABAP) were purchased from the Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Cyanidin-3-O-glucoside chloride was obtained from the Indofine Chemical Company, Inc. (Hillsborough, NJ, USA). Advanced DMEM, phosphate-buffered saline (PBS), Hanks’ balanced salt solution (HBSS), penicillin-streptomycin, and trypsin-EDTA were purchased from Life Technologies (Grand Island, NY, USA). Human colorectal adenocarcinoma (Caco-2) cells were procured from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Liao X., Greenspan P, & Pegg R.B. (2021). Examining the Performance of Two Extraction Solvent Systems on Phenolic Constituents from U.S. Southeastern Blackberries. Molecules, 26(13), 4001.

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NMR Spectroscopy Analysis Procedure

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Proton magnetic resonance (¹H NMR) spectra and carbon magnetic resonance (¹³C NMR) spectra were recorded on a Varian Inova AS400 spectrometer operating at 400 MHz and 100 MHz, respectively. Proton chemical shifts are reported as parts per million (ppm) on the δ scale relative to CDCl₃ (δ 7.26), CD₃OD (δ 3.31) or D₂O (δ 4.79). ¹H NMR spectral data are tabulated in terms of multiplicity of proton absorption (s, singlet; d, doublet; dd, doublet of doublet; t, triplet; q, quartet; m, multiplet; br, broad), coupling constants (Hz), number of protons. Carbon chemical shifts are reported relative to CDCl₃ (δ 77.0) or CD₃OD (δ 49.0). Flash chromatography was performed with ACS grade solvents from Fisher Scientific (Hanover Park, IL). R_f values are quoted for TLC plates of thickness 0.25 mm from Whatman (Florham Park, NJ). The plates were visualized with iodine, dinitrophenylhydrazine or phosphomolybdic acid reagents. For all reactions performed in an inert atmosphere, argon was used unless otherwise specified.

Guo J., Hong L., West X.Z., Wang H, & Salomon R.G. (2016). Bioactive 4-Oxoheptanedioic Monoamide Derivatives of Proteins and Ethanolaminephospholipids: Products of Docosahexaenoate Oxidation. Chemical research in toxicology, 29(10), 1706-1719.

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Toxaphene Analysis in Fish Feeds

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Toxaphene (CAS # 8001-35-2), a mix of congeners was purchased from Chem Service Inc, USA. LMB feeds were purchased from Silvercup Trout Feed, Zeigler Brothers, Inc.,. A.C.S grade solvents, acetonitrile (ACN) and n-hexanes (HEX) were purchased from Fisher Scientific Company.

Martyniuk C.J., Mehinto A.C., Colli-Dula R.C., Kroll K.J., Doperalski N.J., Barber D.S, & Denslow N.D. (2020). Transcriptome and physiological effects of toxaphene on the liver-gonad reproductive axis in male and female largemouth bass (Micropterus salmoides). Comparative biochemistry and physiology. Part D, Genomics & proteomics, 36, 100746.

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BACE1 Enzyme Activity Assay

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Assays were performed according to manufacturer’s instructions. The BACE1 assay kit was purchased from DiscoveRx (Fremont, CA, USA). ACS grade solvents (Fisher Scientific, Waltham, MA, USA) were distilled and filtered before use. HP-20 resin, flash column stationary phases, and HPLC columns were purchased from Supelco (St. Louis, MO, USA), Sorbent Technologies (Atlanta, GA, USA), and Phenomenex (Torrance, CA, USA), respectively.

Neupane R.P., Parrish S.M., Bhandari Neupane J., Yoshida W.Y., Yip M.L., Turkson J., Harper M.K., Head J.D, & Williams P.G. (2019). Cytotoxic Sesquiterpenoid Quinones and Quinols, and an 11-Membered Heterocycle, Kauamide, from the Hawaiian Marine Sponge Dactylospongia elegans. Marine Drugs, 17(7), 423.

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Purification of Organic Solvents

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ACS grade solvents (Fisher Scientific, Waltham, MA, USA) were distilled and filtered before use. HP-20 resin, flash column stationary phases, and HPLC columns were purchased from Supelco (St. Louis, MO, USA), Sorbent Technologies (Atlanta, GA, USA), and Phenomenex (Torrance, CA, USA), respectively.

Dai J., Philbin C.S., Wakano C., Yoshida W.Y, & Williams P.G. (2023). New Nostocyclophanes from Nostoc linckia. Marine Drugs, 21(2), 101.

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NMR Spectroscopy of Organic Compounds

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Proton magnetic resonance (¹H NMR) spectra and carbon magnetic resonance (¹³C NMR) spectra were recorded on a Varian Inova AS400 spectrometer operating at 400 MHz and 100 MHz, respectively. Proton chemical shifts are reported as parts per million (ppm) on the δ scale relative to CDCl₃ (δ 7.26) or CD₃OD (δ 3.31). ¹H NMR spectral data are tabulated in terms of multiplicity of proton absorption (s, singlet; d, doublet; dd, doublet of doublet; t, triplet; q, quartet; m, multiplet; br, broad), coupling constants (Hz), number of protons. Carbon chemical shifts are reported relative to CDCl₃ (δ 77.0) or CD₃OD (δ 49.0). Flash chromatography was performed with ACS grade solvents from Fisher Scientific (Hanover Park, IL). R_f values are quoted for TLC plates of thickness 0.25 mm from Whatman (Florham Park, NJ). The plates were visualized with iodine, dinitrophenylhydrazine or phosphomolybdic acid reagents. For all reactions performed in an inert atmosphere, argon was used unless otherwise specified.

Guo J., Wang H., Hrinczenko B, & Salomon R.G. (2016). Efficient Quantitative Analysis of Carboxyalkylpyrrole Ethanolamine Phospholipids: Elevated Levels in Sickle Cell Disease Blood. Chemical research in toxicology, 29(7), 1187-1197.

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