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Waters 2487 dual λ absorbance detector system

Manufactured by Waters Corporation
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The Waters 2487 dual λ absorbance detector system is a laboratory instrument designed to measure the absorbance of light in liquid samples across two wavelengths simultaneously. It is capable of detecting and quantifying various analytes in a sample by measuring their absorption of specific wavelengths of light.

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Lab products found in correlation

1525 binary hplc pump, by Waters Corporation (3 mentions) 1600 series ftir spectrometer, by PerkinElmer (3 mentions) Arx300 300 mhz nmr spectrometer, by Bruker (2 mentions) Waters 1525 binary hplc pump, by Waters Corporation (1 mentions) Drx500 500 mhz, by Bruker (1 mentions) Drx 2 spectrometer, by Bruker (1 mentions) Estrogen receptor α and β competitor assay kits, by Thermo Fisher Scientific (1 mentions) Arx300 spectrometer, by Bruker (1 mentions)

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4 protocols using waters 2487 dual λ absorbance detector system

1

Spectroscopic Analysis of Chemical Compounds

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Melting points were determined with a Mel-Temp apparatus using capillary tubes and are uncorrected. Proton nuclear magnetic resonance spectra (1H NMR) were recorded using an ARX300 300 MHz Bruker NMR spectrometer. IR spectra were obtained with a PerkinElmer 1600 series FTIR spectrometer. The purities of all of the biologically tested compounds were estimated by HPLC, and in each case, the major peak accounted for ≥95% of the combined total peak area when monitored by a UV detector at 254 nm. HPLC analyses were performed on a Waters 1525 binary HPLC pump/Waters 2487 dual λ absorbance detector system. HPLC analyses were performed on a Sunrise C-18 column with dimensions of 16 × 4.6 cm and 5 μm particle size. Analytical thin-layer chromatography was conducted on Baker-flex silica gel IB2-F plates, and compounds were visualized with UV light at 254 nm. Silica gel flash chromatography was performed using 230–400 mesh silica gel. The anhydrides 11a and 11b and compound 12 were prepared according to the literature and showed similar spectroscopic data.38 (link),45
Elsayed M.S., Su Y., Wang P., Sethi T., Agama K., Ravji A., Redon C.E., Kiselev E., Horzmann K.A., Freeman J.L., Pommier Y, & Cushman M. (2017). Design and Synthesis of Chlorinated and Fluorinated 7-Azaindenoisoquinolines as Potent Cytotoxic Anticancer Agents That Inhibit Topoisomerase I. Journal of medicinal chemistry, 60(13), 5364-5376.
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2

Analytical Characterization of Organic Compounds

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Melting points were determined with a Mel-Temp apparatus using capillary tubes and are uncorrected. The proton nuclear magnetic resonance spectra (1H NMR) were recorded using an ARX300 300 MHz Bruker NMR spectrometer. IR spectra were obtained with a Perkin-Elmer 1600 series FTIR spectrometer. For purities estimated by HPLC, the major peak accounted for ≥ 95% of the combined total peak area when monitored by a UV detector at 254 nm. HPLC analyses were performed on a Waters 1525 binary HPLC pump/Waters 2487 dual λ absorbance detector system. Analytical thin-layer chromatography was conducted on Baker-flex silica gel IB2-F plates, and compounds were visualized with UV light at 254 nm. Silica gel flash chromatography was performed using 230–400 mesh silica gel.
Elsayed M.S., Chang S, & Cushman M. (2017). Ligand-free, palladacycle-facilitated Suzuki coupling of hindered 2-arylbenzothiazole derivatives yields potent and selective COX-2 inhibitors. Organic & biomolecular chemistry, 16(1), 108-118.
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3

Characterization of Organic Compounds

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Melting points were determined with a Mel-Temp apparatus using capillary tubes and are uncorrected. The proton nuclear magnetic resonance spectra (1H NMR) were recorded using Bruker ARX300 300 MHz or Bruker DRX500 500 MHz spectrometers. IR spectra were obtained with a PerkinElmer 1600 series FTIR spectrometer. For biologically tested compounds, the HPLC major peak accounted for ≥95% of the combined total peak area when monitored by a UV detector at 254 nm. HPLC analyses were performed on a Waters 1525 binary HPLC pump/Waters 2487 dual λ absorbance detector system. Analytical thin-layer chromatography was conducted on Baker-flex silica gel IB2-F plates, and compounds were visualized with UV light at 254 nm. Silica gel flash chromatography was performed using 230−400 mesh silica gel.
Elsayed M.S., Nielsen J.J., Park S., Park J., Liu Q., Kim C.H., Pommier Y., Agama K., Low P.S, & Cushman M. (2018). Application of Sequential Palladium Catalysis for the Discovery of Janus Kinase Inhibitors in the Benzo[c]pyrrolo[2,3‑h][1,6]naphthyridin-5-one (BPN) Series. Journal of medicinal chemistry, 61(23), 10440-10462.
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4

Compound Characterization and Screening

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Melting points were determined using capillary tubes with a Mel-Temp apparatus and are uncorrected. The nuclear magnetic resonance (1H and 13C NMR) spectra were recorded using a Bruker ARX300 spectrometer (300 MHz) with a QNP probe or a Bruker DRX-2 spectrometer (500 MHz) with a BBO probe. High-resolution mass spectra were recorded on a double-focusing sector mass spectrometer with magnetic and electrostatic mass analyzers. The purities of biologically tested compounds are ≥ 95% as determined by HPLC or elemental analyses. For elemental analyses, the observed percentages differ less than 0.40% from the calculated values. For purities estimated by HPLC, the major peak accounted for ≥ 95% of the combined total peak area when monitored by a UV detector at 254 nm. The HPLC analyses were performed on a Waters 1525 binary HPLC pump/Waters 2487 dual λ absorbance detector system using a 5 μm C18 reversed phase column. Cytochrome P450 (CYP) inhibitor screening kits for aromatase (CYP19) inhibition studies were purchased from BD Biosciences (San Jose, CA). Estrogen receptor α and β competitor assay kits were purchased from Invitrogen (Carlsbad, CA).
Lv W., Liu J., Skaar T.C., Flockhart D.A, & Cushman M. (2015). Design and Synthesis of Norendoxifen Analogues with Dual Aromatase Inhibitory and Estrogen Receptor Modulatory Activities. Journal of medicinal chemistry, 58(6), 2623-2648.
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