Skin swab cultures (sterile transport swab on Stuart agar gel medium, Copan
Venturi Transystem®; Copan, Murrieta, CA, USA) were performed at suspected sites of skin infection and then inoculated on blood agar plates followed by McConkey agar plates. They were subsequently incubated in a carbon dioxide (CO
2) incubator set at 35℃ and 5% CO
2 for 1 day (Thermo Scientific Inc., Waltham, MA, USA). Gram staining was performed in the presence of colonies on inoculated culture medium. Gram-positive bacteria were judged to be
S. aureus when their catalase and coagulase tests were positive, and CoNS was determined according to negative catalase and coagulase results. Subsequently, the specimens were inoculated on Mueller-Hinton agar and tested for susceptibility to antibiotics by disk diffusion method.
For other species, automation equipment, including
VITEK 2 (bioMerieux Inc., Hazelwood, MO, USA) and
MicroScan (Beckman Coulter Inc., Brea, CA, USA), was used for microbial identification and antibiotic susceptibility tests.
Lim J.S., Park H.S., Cho S, & Yoon H.S. (2018). Antibiotic Susceptibility and Treatment Response in Bacterial Skin Infection. Annals of Dermatology, 30(2), 186-191.