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Venturi transystem

Manufactured by Copan
Sourced in Italy, United States

The Venturi Transystem is a laboratory equipment product that utilizes the Venturi effect to create a vacuum or suction force. Its core function is to provide a reliable and controlled air flow for various laboratory applications.

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3 protocols using venturi transystem

1

Surveillance of colonization by MDRO

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Swabs were performed using sterile transport swab (ref. 139C) on specific media (liquid Stuart, Venturi Transystem® by COPAN Italia, Brescia, Italy).
Each swab was cultivated on specific selective media (chromID® VRE and chromID® CARBA SMART by bioMérieux, Marcy l’Etoile, France), and on regular MDRO agar plates according to manufacturer. No enrichment cultures were used.
Moreover, at admission colonization was systematically confirmed by PCR testing (PCR Xpert CarbaR and Xpert VanA/VanB by Cepheid, Maurens-Scopont®, France) and during follow-up in case of negative culture on the demand of physician.
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2

Skin Infection: Microbial Identification and Antibiotic Susceptibility

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Skin swab cultures (sterile transport swab on Stuart agar gel medium, Copan Venturi Transystem®; Copan, Murrieta, CA, USA) were performed at suspected sites of skin infection and then inoculated on blood agar plates followed by McConkey agar plates. They were subsequently incubated in a carbon dioxide (CO2) incubator set at 35℃ and 5% CO2 for 1 day (Thermo Scientific Inc., Waltham, MA, USA). Gram staining was performed in the presence of colonies on inoculated culture medium. Gram-positive bacteria were judged to be S. aureus when their catalase and coagulase tests were positive, and CoNS was determined according to negative catalase and coagulase results. Subsequently, the specimens were inoculated on Mueller-Hinton agar and tested for susceptibility to antibiotics by disk diffusion method.
For other species, automation equipment, including VITEK 2 (bioMerieux Inc., Hazelwood, MO, USA) and MicroScan (Beckman Coulter Inc., Brea, CA, USA), was used for microbial identification and antibiotic susceptibility tests.
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3

Demographic and Risk Factors Survey

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After informed consent and assent (when appropriate) were obtained, participants and legal guardians were administered a survey pertaining to their demographic, personal and household members’ risk factors (Table 1). Two swabs were then collected, one each from the anterior nares and axillae. For the nares, a cotton-tipped swab (Copan Venturi Transystem® with Liquid Stuart Medium) was rotated three times in the anterior portion of each naris. For the axilla, three to five brush strokes under each axillary area were taken. Moistened swabs were then transported immediately to the clinical microbiology laboratory for plating on selective and non-selective media. The institutional review boards of participating institutions approved this study.
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