Au5800

Mice blood was collected from mice via the inferior vena cava with a heparin-coated microhaematocrit tube and centrifugation for 15 min at 2,000 × g to obtain plasma. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured using an automatic chemical analyser (Olympus Company AU5800, Tokyo, Japan).

The T1 and T2 blood samples were collected during hospitalization, while the T3 and T4 blood samples were collected during the outpatient follow-up. All samples were collected in the morning from patients who fasted. Serum samples were centrifuged at 3500 rpm for 5 min and biochemical parameters were performed with an AU5800 (OLYMPUS, Japan). EDTA-K2 anticoagulant samples were measured using a Sysmex 2100D routine hematology analyzer (Sysmex, Japan) for routine blood examinations. EDTA-K2 anticoagulant samples were measured using a FACSCanto II flow cytometer (BD, USA) for cytokines. Normal reference range for adult liver function: ALT: male: 9–50 (U/L), female: 7–40 (U/L); AST: male: 15–40 (U/L), female: 13–35 (U/L); ALP: male: 45–125 (U/L), female: 35–135 (U/L); GGT: male: 10–60 (U/L), female: 7–45 (U/L); TB: male and female: 3.4–20.5 (μmol/L). Based on previously published articles,11 (link) anything beyond the upper limit reference range for liver function was defined as abnormal liver function.