Goat anti cpa1

For quantification of ADM and AFLs, random whole pancreatic sections stained with hematoxylin and eosin H&E were analyzed. For immunohistochemical staining, sections of paraffin embedded pancreata were rehydrated and antigen retrieval was performed using Antigen Unmasking Solution (Vector Laboratories). Overnight incubation with the following primary antibodies was done at 4°C: goat anti-CPA1 (R&D), rabbit anti-CK19 (Abcam), rabbit anti-GFP (Santa Cruz Biotechnology), mouse anti-Ki67 (BD), rabbit anti-P53 (Vector Laboratories), goat anti-Amylase (Santa Cruz Biotechnology), rabbit anti-p44/p42 MAPK (Cell Signaling) and FITC-conjugated DBA-lectin (Vector Laboratories). Biotin-conjugated secondary antibodies were incubated for 1 h at room temperature, following development with ABC and DAB kits (both Vector Laboratories). Nuclear counterstaining was performed using haematoxylin. For immunofluorescence, sections were incubated with fluorophore-conjugated secondary antibody for 1 h at room temperature. Slides were mounted with DAPI hardset antifade mounting medium.