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Sc 1473

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

sc-1473 is a laboratory equipment product provided by Santa Cruz Biotechnology. It serves a core function in research and scientific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Sc 568, by Santa Cruz Biotechnology (1 mentions) Sc 569, by Santa Cruz Biotechnology (1 mentions) Ecl detection system, by GE Healthcare (1 mentions) Quantity one, by Bio-Rad (1 mentions) Gapdh ta 08, by ZSGB-BIO (1 mentions) Goat anti rabbit sc 2004, by Santa Cruz Biotechnology (1 mentions) Secondary horseradish peroxidase conjugated igg antibodies, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat sc 2020, by Santa Cruz Biotechnology (1 mentions) Sc 570, by Santa Cruz Biotechnology (1 mentions) Sc 32233, by Santa Cruz Biotechnology (1 mentions) 3h cgp12177, by PerkinElmer (1 mentions) Ab129068, by Abcam (1 mentions) Ab3442, by Abcam (1 mentions) Ab209794, by Abcam (1 mentions) Ab3462, by Abcam (1 mentions) Ab22554, by Abcam (1 mentions) Ab182136, by Abcam (1 mentions) Mab318, by Merck Group (1 mentions) Sc 7020, by Santa Cruz Biotechnology (1 mentions) Sc 271968, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sc 1473

1

Quantitative Western Blot Analysis

Cited 1 time
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Protein extracts (20 μg) were separated on SDS/PAGE (12% gel) as described previously [15 (link)]. Blocked membranes were incubated with the antibodies against β1-AR (sc-568), β2-AR (sc-569), β3-AR (sc-1473), p-PKA (Thr198) (sc-32968), and PKAα (sc-28315) at 1:500 dilution (Santa Cruz Biotechnology, Delaware, CA, U.S.A.); against VAMP-2 (104 211, Synaptic Systems, Göttingen, Germany), at 1:5000 dilution; and against syntaxin-4 (ab77037; Abcam, Hong Kong, China), occludin (33-1500; Invitrogen, Carlsbad, CA, U.S.A.), and GAPDH (TA-08; ZSGB-BIO, Beijing, China) at 1:1000 dilution in TBS-T (20 mM Tris, 137 mM NaCl, 0.1% Tween-20, pH 7.4). Membranes were washed and probed with horseradish peroxidase-conjugated secondary antibodies (1:8000) and developed in an ECL detection system (GE Biosciences, Buckinghamshire, England). Densitometry data were analyzed by Quantity One (Bio–Rad, Hercules, CA).
Ding C., Cong X., Zhang Y., Li S.L., Wu L.L, & Yu G.Y. (2018). β-adrenoceptor activation increased VAMP-2 and syntaxin-4 in secretory granules are involved in protein secretion of submandibular gland through the PKA/F-actin pathway. Bioscience Reports, 38(1), BSR20171142.
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2

β-Adrenergic Receptor Quantification

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[3H]CGP12177 (specific activity 41.6 Ci/mmol) was purchased from Perkin Elmer Health Sciences (Toronto, ON, Canada). Antibodies against rat β1-AR (Ab3546) were purchased from AbCam (Cambridge, MA, USA). Antibodies against β2-AR (SC-570), β3-AR (SC-1473), and GAPDH (SC-32233) were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA, USA). Secondary horseradish peroxidase conjugated IgG antibodies goat anti-rabbit (SC-2004), donkey anti-goat (SC-2020), and donkey anti-mouse (SC-2314) were also purchased from Santa Cruz Biotechnology.
Haley J.M., Thackeray J.T., Thorn S.L, & DaSilva J.N. (2015). Cardiac β-Adrenoceptor Expression Is Reduced in Zucker Diabetic Fatty Rats as Type-2 Diabetes Progresses. PLoS ONE, 10(5), e0127581.
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3

Antibody Characterization in Animal Studies

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Primary antibodies used in animal studies were listed as follows: Na+/K+-ATPase α 1 subunit (05–369, Millipore, CA, USA), glucose transporter 4 (Glut4, 2213, Cell Signaling Technology, MA, USA), insulin receptor substrate 1 (IRS1, sc-559, TX, Santa Cruz Biotechnology), phosphotyrosine (sc-7020, Santa Cruz Biotechnology, USA), phosphorylated AS160 (Tyr642) (4288, Cell Signaling Technology), total AS160 (07–741 Millipore), tumor necrosis factor (TNFα, ab6671, Abcam, MA, USA), ED-1 (MCA341, Serotec, Oxford, UK), angiotensinogen (AGT, 28101, IBL, Gunma, Japan), AGT (A6279, ABclonal, Wuhan, China), Ang II (T-4007, Peninsula Laboratories, CA, USA), dystrophin (ab129996, Abcam), adiponectin (ab22554, Abcam), CGRP (GTX16211, Gene Tex, CA, USA), tyrosine hydroxylase (TH, MAB318, Millipore), c-fos (ab209794, Abcam), Nox2 (ab129068, Abcam), p22phox (sc-271,968, Santa Cruz Biotechnology), α1-adrenergic receptor (AR) (ab3462, Abcam), α2-adrenergic AR (CA1003, Cell Application, CA, USA), β1-AR (ab3442, Abcam), β2-AR (ab182136, Abcam), β3-AR (sc-1473, Santa Cruz Biotechnology). Secondary antibodies were purchased from Cell Signaling Technology.
Cao W., Shi M., Wu L., Yang Z., Yang X., Liu H., Xu X., Liu Y., Wilcox C.S, & Hou F.F. (2018). A renal-cerebral-peripheral sympathetic reflex mediates insulin resistance in chronic kidney disease. EBioMedicine, 37, 281-293.
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