Spot rt digital camera

Manufactured by Nikon

Sourced in Japan

The Spot RT digital camera is a high-quality imaging device designed for use in various laboratory applications. It features a CCD sensor that captures detailed digital images with a resolution suitable for scientific and research purposes. The Spot RT camera is capable of producing images with accurate color representation and high sensitivity, making it a versatile tool for a range of laboratory tasks.

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Digital camera (372 citations) Spot digital camera (4 citations) Spot RT Slider digital camera (3 citations) SPOT RTTM digital camera (2 citations)

12 protocols using spot rt digital camera

Fibroblast Adhesion and Outgrowth

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NIH3T3 fibroblasts (20,000/cm²) were cultured overnight on peptide-functionalized or unmodified PEGDA hydrogels. Cells were labeled with the Live/Dead assay (Invitrogen) and fluorescence images were acquired in a Nikon TE-300 microscope with a 20X objective and Spot RT digital camera. Cell densities were quantified using in-house macros in ImageJ.
IMR-90 fibroblasts were detached, pelleted, and pipetted into polymerizing PEG-maleimide gels to encapsulate the cell pellet within gel. After 30 minutes, cell media was added. Cell outgrowth sprout length was analyzed at 24 hours post-encapsulation using ImageJ.

Lee T.T., García J.R., Paez J., Singh A., Phelps E.A., Weis S., Shafiq Z., Shekaran A., del Campo A, & García A.J. (2014). Light-triggered in vivo Activation of Adhesive Peptides Regulates Cell Adhesion, Inflammation and Vascularization of Biomaterials. Nature materials, 14(3), 352-360.

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Visualizing CD44 Expression in Ovarian Cancer

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Immunofluorescence assay was utilized for visualizing the post transfection expression levels of CD44 in ovarian cancer cells. Firstly, the 48-hour post transfection cells were incubated in 4% paraformaldehyde, fixed in ice-cold methanol, and blocked with 1% bovine serum albumin (BSA). Then, immunostaining was performed using the CD44 antibody, Alexa Fluor 594 (Red) conjugated goat anti-mouse antibody (Life Technologies), and 1 μg/ml Hoechst 33342 (Life Technologies) for counterstaining of nuclei. Finally, the cells were photographed on a Nikon Eclipse Ti-U fluorescence microscope (Nikon Instruments, Inc NY, CA) equipped with a SPOT RT™ digital camera. Red color represented CD44 protein and blue color represented cell nuclei.

Gao Y., Foster R., Yang X., Feng Y., Shen J.K., Mankin H.J., Hornicek F.J., Amiji M.M, & Duan Z. (2015). Up-regulation of CD44 in the development of metastasis, recurrence and drug resistance of ovarian cancer. Oncotarget, 6(11), 9313-9326.

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Slug Protein Expression in Chordoma Cells

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Expression of Slug protein was also evaluated by immunofluorescence. In brief, chordoma cell line UCH1 was transfected with pre-miR-1 and NS-miRNA for 48h. The cells were then incubated in 4% paraformaldehyde, and fixed in ice-cold methanol and blocked with 1 % bovine serum albumin (BSA), and bound with Slug (1:50) and actin (1:400) antibodies. The cells were incubated with anti-rabbit IgG (1:1000), anti-mouse IgG (1:1000) and Hoechst 33342 (Life Technologies Corp, NY). The cells were captured on a Nikon Eclipse Ti-U fluorescence microscope (Nikon Instruments Inc, NY) equipped with a SPOT RT^™ digital camera.

Osaka E., Yang X., Shen J.K., Yang P., Feng Y., Mankin H.J., Hornicek F.J, & Duan Z. (2014). MicroRNA-1(miR-1) inhibits chordoma cell migration and invasion by targeting Slug. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 32(8), 1075-1082.

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Visualizing Intracellular Accumulation of Pgp Substrates

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For visualisation of the intracellular accumulation level of Pgp substrate Calcein AM and Rhodamine 123 (R123) in different U-2OS cell sublines, 2 × 10⁴ cells were seeded in 12-well plate in duplicate the day before the assay. Cells were then incubated with either 0.25 μM Calcein AM or 1 μM R123 in RPMI-1640 medium for 2 h at 37 °C. After washing the cells with PBS, 1 μg ml⁻¹ Hoechst 33342 was used to stain nuclei. Images were acquired by Nikon Eclipse Ti-U fluorescence microscope equipped with a SPOT RT digital camera.

Yang X., Yang P., Shen J., Osaka E., Choy E., Cote G., Harmon D., Zhang Z., Mankin H., Hornicek F.J, & Duan Z. (2014). Prevention of multidrug resistance (MDR) in osteosarcoma by NSC23925. British Journal of Cancer, 110(12), 2896-2904.

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Visualizing CDK9 Expression in Chordoma Cells

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The expression of CDK9 in chordoma cells was visualized via immunofluorescence assay as described previously 21 (link). The UCH2 and CH22 cells were first seeded into 24-well plates before being incubated in 4% paraformaldehyde, fixed with ice-cold methanol, and blocked with 1% bovine serum albumin. Immunostaining was performed with CDK9 (1:200 dilution) and β-actin (1:500 dilution, Sigma-Aldrich, St. Louis, MO). After being washed three times with PBS, the cells were incubated with Alexa Fluor 594 (red) goat anti-mouse antibody and Alexa Fluor 488 (green) conjugated goat anti-rabbit antibody (Invitrogen) for one hour. Images were obtained with a Nikon Eclipse Ti-U fluorescence microscope equipped with a SPOT RT™ digital camera. Green color highlights CDK9 protein, and red color highlights the cytoplasm.

Shen S., Dean D.C., Yu Z., Hornicek F., Kan Q, & Duan Z. (2020). Aberrant CDK9 expression within chordoma tissues and the therapeutic potential of a selective CDK9 inhibitor LDC000067. Journal of Cancer, 11(1), 132-141.

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Histological Analysis of Chagas Disease

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Hearts from untreated uninfected controls, T. cruzi-infected and T. cruzi-infected and fenofibrate-treated IL-10 KO and WT mice were fixed in PBS-buffered 4% paraformaldehyde and included in paraffin after dehydration. Six non-contiguous sections (5 μm) were stained with hematoxylin-eosin or picrosirius red for the examination of cellular infiltrates and collagen deposits. Images from thirty random microscopic fields (400x) were acquired using an Eclipse E600 microscope (Nikon Inc.) equipped with a Spot RT digital camera. Analysis was performed using the Image J software (NIH, United States) (38 (link)).

Rada J., Donato M., Penas F.N., Alba Soto C., Cevey Á.C., Pieralisi A.V., Gelpi R., Mirkin G.A, & Goren N.B. (2020). IL-10-Dependent and -Independent Mechanisms Are Involved in the Cardiac Pathology Modulation Mediated by Fenofibrate in an Experimental Model of Chagas Heart Disease. Frontiers in Immunology, 11, 572178.

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Fibroblast Adhesion and Outgrowth

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Histological Analysis of Mouse Hearts

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Mouse hearts were fixed in PBS-buffered 4% formaldehyde and included in paraffin. Six non-contiguous sections (5 μm) were stained with hematoxylin-eosin or picrosirius red. Images from thirty random microscopic fields (400x) were acquired using an Eclipse E600 microscope (Nikon Inc.) equipped with a Spot RT digital camera. Image analysis was performed using the Image J software (NIH, USA).

Cevey Á.C., Mirkin G.A., Donato M., Rada M.J., Penas F.N., Gelpi R.J, & Goren N.B. (2017). Treatment with Fenofibrate plus a low dose of Benznidazole attenuates cardiac dysfunction in experimental Chagas disease. International Journal for Parasitology: Drugs and Drug Resistance, 7(3), 378-387.

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Evaluating ATR's Role in 3D Ovarian Cancer Cell Growth

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The 3D cell culture system mimics the in vivo environment and serves as a unique platform to evaluate how ATR is related to in vivo ovarian cancer cell growth. Consistent with the manufacturer’s protocol, the ovarian cancer cell lines SKOV3 and OVCAR8 were mixed with 3D VitroGel™ (TheWell Bioscience Inc., North Brunswick Township, NJ, USA) then established in 24-well plates at a density of 1 × 10⁴ cells/ml. Each well was covered with the same volume of cell culture medium. The experimental group received an additional treatment of VE-822 at concentration of 0.1 μM. The plates were then placed in a 37°C incubator with a humidified 5% CO₂ atmosphere and the covering medium was changed every 48 h. Images of the cell spheroids were obtained with a Nikon microscope every 3 days. After 15 days, calcein-AM (Thermo Fisher Science) was applied to stain the tumor spheroids, and images were obtained with an Eclipse Ti-U fluorescence microscope (Nikon) equipped with a Spot RT digital camera.

Feng W., Dean D.C., Hornicek F.J., Wang J., Jia Y., Duan Z, & Shi H. (2020). ATR and p-ATR are emerging prognostic biomarkers and DNA damage response targets in ovarian cancer. Therapeutic Advances in Medical Oncology, 12, 1758835920982853.

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DRGNs Dye Uptake Assay

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DRGNs were seeded in monolayers on glass-bottomed dishes (MatTek, USA) at a density of 2000 cells/cm², and then cultured with DMEM medium (#8,121,513, Gibco, USA) with 10% FBS (#10,099,141, Gibco, USA) for 5 d. After rinsing twice with PBS without Ca²⁺ and Mg²⁺ (#21-031-CV, Corning, USA), DRGNs were incubated for 10 min in 5 ml of DMEM with 10 µmol/L EtBr (#E7637, Merck, Germany) and 2 mmol/L ATP (#R0441, Thermo Scientific, USA). The fluorescence intensity of EtBr dye uptake was monitored using a Nikon Eclipse TE300 microscope (Nikon Co., Minato, TY, Japan) and Spot-RT digital camera with fixed gain and exposure duration.

Xing Q., Cibelli A., Yang G.L., Dohare P., Li Q.H., Scemes E., Guan F.X, & Spray D.C. (2024). Neuronal Panx1 drives peripheral sensitization in experimental plantar inflammatory pain. Military Medical Research, 11, 27.

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