Difco agar

Manufactured by Thermo Fisher Scientific

Sourced in United States

Difco Agar is a solidifying agent commonly used in microbiological culture media. It is derived from red seaweed and provides a gelled medium for the growth and isolation of microorganisms.

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Lab products found in correlation

2 protocols using difco agar

Synthesis and Characterization of Antimicrobial Nanoparticles

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Zinc chloride (ZnCl₂), iron chloride hexahydrate (FeCl₃.6H₂O), sodium hydroxide (NaOH), oleic acid, and nutrient agar were purchased from Daejung Korea (Nakdong-daero, Sasang-gu, Busan, Korea). Dulbecco’s Modified Eagle’s Medium (DMEM containing L-Glutamine and 4.5 g/L Glucose), fetal bovine serum (FBS), 100X Penicillin-Streptomycin (Pen/Strep), 0.25 % trypsin-EDTA, silver sulfadiazine, Tetracycline hydrochloride, dimethyl sulfoxide (DMSO), Drabkin’s reagent, pure human hemoglobin and bovine serum albumin (BSA) were bought from Sigma-Aldrich (Saint Louis, MO, USA). Bacto tryptic soy broth, Difco Agar, Lb Agar, Live/dead viability/cytotoxicity kit, PrestoBlue dye, polyethylene glycol (PEG), live/dead BacLight bacterial viability kit, and fluorescein isothiocyanate fluorescent dye (FITC) were obtained from Fisher Scientific (Waltham, MA, USA). Tetracycline, Bio-Rad Protein reagent, and dichlorofluorescein diacetate (DCFDA) dye were acquired from Bio-Rad Laboratories (Hercules, CA, USA). NIH-3T3 murine fibroblast, human dermal fibroblast (HDF) cells, and bacterial cultures were provided by American Type Culture Collection (ATCC, Manassas, VA, USA).

Haghniaz R., Rabbani A., Vajhadin F., Khan T., Kousar R., Khan A.R., Montazerian H., Iqbal J., Libanori A., Kim H.J, & Wahid F. (2021). Anti‐bacterial and wound healing‐promoting effects of zinc ferrite nanoparticles. Journal of Nanobiotechnology, 19, 38.

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Synthesis and Purification of Tryptophan Derivatives

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Benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP), L-tryptophan methyl ester hydrochloride, N,N-diisopropylethylamine (DIPEA), dichloromethane (DCM), hepes, magnesium chloride hexahydrate, iron (II) chloride and sodium chloride were purchased from Sigma-Aldrich (St. Louis, MO). Methanol (MeOH), acetonitrile (ACN), celite, C18 reverse phase silica gel, tryptic soy broth (TSB), Luria–Bertani broth (LB), Difco agar, sucrose, potassium sulfate, glucose, casamino acids, yeast extract, MOPS, and trace elements were purchased from Fisher Scientific (Hampton, NH). Methanol-d4 was purchased from Cambridge Isotope Laboratories Inc. (Boston, MA, USA). S. argillaceus ATCC 12956 was purchased from ATCC (Manassas, VA). DNA oligomers were purchased from Integrated DNA Technologies, Inc. (IDT) (Coralville, IA). Microtiter plates were purchased from Thermo Fisher (Waltham, MA). MTM, MTM SDK, PreMTM B, and MTM SA Ala, were produced by previously reported procedures [17 (link),19 ,31 (link)].

Weidenbach S., Hou C., Chen J.M., Tsodikov O.V, & Rohr J. (2015). Dimerization and DNA recognition rules of mithramycin and its analogues. Journal of inorganic biochemistry, 156, 40-47.

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