α myc 9e10

Manufactured by Santa Cruz Biotechnology

α-Myc (9E10) is a mouse monoclonal antibody that recognizes the c-Myc protein. It is a useful tool for the detection and purification of c-Myc and Myc-tagged recombinant proteins.

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Lab products found in correlation

Secondary peroxidase conjugated goat α rabbit mouse antibodies, by Merck Group (1 mentions) Alexa fluor 594 conjugated goat α mouse rabbit antibodies, by Thermo Fisher Scientific (1 mentions) Chemiluminescent hrp substrate, by Merck Group (1 mentions) Bis tris gel, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Dynabeads protein a, by Thermo Fisher Scientific (1 mentions) α β actin, by Merck Group (1 mentions) αha 16b12, by BioLegend (1 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (1 mentions) Abe572, by Merck Group (1 mentions) αmettl3, by Proteintech (1 mentions) Trans blot turbo transfer kit, by Bio-Rad (1 mentions)

Close spelling variants of this product

Myc (9E10) α-Myc

4 protocols using α myc 9e10

Antibody Characterization in Toxoplasma

Cited 3 times

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The antibodies used in this study were previously described as follow: polyclonal rabbit: α-MyoA and α-MLC1 (ref. 59 (link)), α-GAP40, α-IMC1 and α-GAP45 (ref. 9 (link)), α-MLC2 (ref. 28 (link)), α-Cpn60 (ref. 32 (link)), α-catalase60 (link), α-ARO12 (link), α-HSP70 (ref. 40 (link)); monoclonal mouse, α-ACT59 (link), α-ISP1 (ref. 61 (link)), α-Ty (BB2, ref. 62 (link)). α-SAG1, α-MIC2, α-GRA1, α-GRA3 and α-P21 are generous gifts from Dr J.-F. Dubremetz. For WB, secondary peroxidase-conjugated goat α-rabbit/mouse antibodies (Sigma) were used, as well as α-Myc (9E10, Santa Cruz Sc-40). For IFA, the secondary antibodies Alexa Fluor 488- and Alexa Fluor 594-conjugated goat α-mouse/rabbit antibodies (Life Technologies) as well as the DBA-fluorescein labelled (Reactolab FL-1031) were used. The dilutions of use are described in Supplementary Table 3.

Frénal K., Jacot D., Hammoudi P.M., Graindorge A., Maco B, & Soldati-Favre D. (2017). Myosin-dependent cell-cell communication controls synchronicity of division in acute and chronic stages of Toxoplasma gondii. Nature Communications, 8, 15710.

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Western Blot of Parasite Proteins

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Western blot was performed as previously described^{51 (link)}. Lysate from 5×10⁶ parasites was loaded in 12% Bis-Tris gel (Invitrogen), transferred to PVDF membrane (Bio-rad), blocked in 5% milk and probed with rabbit α-human GAPDH antibody conjugated with horse radish peroxidase (HRP) (TGR BioSciences) at 1:500, or α-Myc (9E10) conjugated with HRP (Santa Cruz Biotechnologies) or α-IMC1. Following 1 hr incubation the membrane was washed 3 times with PBS containing 0.1% Tween40 for 10 min and once with PBS for 5 min. Signals were detected using chemiluminescent HRP substrate (Millipore) and exposure on X-ray film.

Dubey R., Staker B.L., Foe I.T., Bogyo M., Myler P.J., Ngô H.M, & Gubbels M.J. (2016). Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1. Molecular microbiology, 103(4), 618-634.

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Comprehensive Antibody Characterization for Molecular Biology

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Antibodies used in this study include αm⁶A (Millipore, ABE572 or MABE1006) for MeRIP/m⁶A-seq and MeRIP-qPCR, αHMBOX1 (Novus Biologicals, NBP1-31316) for ChIP-qPCR and immunoblotting, αγH2AX (JBW301, Millipore, 05-636) for immunofluorescence-FISH and Western blot, αTPP1 (Proteintech, 25849-1-AP) for immunoprecipitation (IP) and immunoblotting, and αTRF2 (Proteintech, 66893-1-Ig) for immunofluorescence-FISH. Other antibodies that were applied in Western blot analysis include αMETTL3 (Proteintech, 15073-1-AP), αMETTL14 (Sigma-Aldrich, HPA038002), αALKBH5 (Sigma-Aldrich, HPA007196), αHA (16B12) (BioLegend, #901501), αmyc (9E10) (Santa Cruz Biotechnology, sc-40), αHis (Proteintech, #66005-1), αCRISPR-Cas9 (Abcam, ab191468), αAR (H-280) (Santa Cruz Biotechnology, sc-13062), αYTHDF2 (Proteintech, 24744-1-AP), αYTHDC2 (Proteintech, 27779-1-AP), αp53 (DO-1) (Santa Cruz Biotechnology, sc-126), αp21 (Cell Signaling Technology, #2947), αMDM2 (Proteintech, 19058-1-AP), αTelomerase reverse transcriptase (TERT) (Abcam, ab32020), and αβ-actin (Sigma-Aldrich, A5441). Reagents that were used in IP, ChIP, and MeRIP are Protein A/G Plus Agarose (Santa Cruz Biotechnology, sc-2003) and Dynabeads Protein A (Thermo Fisher Scientific, #10006D) or Protein G (Thermo Fisher Scientific, #10007D) immunoprecipitation kit.

Lee J.H., Hong J., Zhang Z., de la Peña Avalos B., Proietti C.J., Deamicis A.R., Guzmán G. P., Lam H.M., Garcia J., Roudier M.P., Sisk A.E., De La Rosa R., Vu K., Yang M., Liao Y., Scheirer J., Pechacek D., Yadav P., Rao M.K., Zheng S., Johnson-Pais T.L., Leach R.J., Elizalde P.V., Dray E, & Xu K. (2021). Regulation of telomere homeostasis and genomic stability in cancer by N6-adenosine methylation (m6A). Science Advances, 7(31), eabg7073.

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Western Blot Analysis of Epitope-Tagged Proteins

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Western blots were performed as described previously [61 (link)]. Following SDS-PAGE, proteins were transferred onto PVDF membrane using Trans-Blot Turbo Transfer Kit (Biorad) and blocked in 5% milk in TBS-T (50mM Tris-HCl, 150mM NaCl, 0.1% Tween20, pH 8.0) at 4°C for at least 1 hour. Respective primary HRP-conjugated antibodies (α-FLAG: Cohesion Biosciences, CPA9020; α-HA: Invitrogen, #26183-HRP; α-V-5: Invitrogen, #MA5-15253-HRP; α-Myc (9E10): Santa Cruz Biotechnology, SC-40) were applied for overnight incubation (4°C). Membranes were then rinsed with TBS-T. Proteins were detected using ECL Extreme reagents (Expedeon) in chemiluminescence CCD camera (ImageQuant LAS 500).

Zdrzałek R., Kamoun S., Terauchi R., Saitoh H, & Banfield M.J. (2020). The rice NLR pair Pikp-1/Pikp-2 initiates cell death through receptor cooperation rather than negative regulation. PLoS ONE, 15(9), e0238616.

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