Ccd mega view camera

One microlitre loop of 20 h old biofilms grown on silicone, polystyrene (cell culture dishes) and K. pneumoniae cultures in MH broth were spotted onto LB agar plate containing 25 mg/mL Congo red (Sigma). Cultures were incubated for 72 h at 37 °C for colony morphology analysis as described before [14 (link)]. Colonies were further analysed by transmission electron microscopy (TEM) under a Morgagni 268D TEM (FEI Electron Optics, Hillsboro, OR, USA) after negative staining with 0.5% uranyl acetate (EMS, Hatfield, PA, USA) in water for 5 min at room temperature. Digital images were acquired with a CCD Mega-View camera (Olympus Soft Imaging Solutions GmbH, Munich, Germany).

Three-month-old C57BL6/J mice or twelve-month-old albino mice were transcardially perfused with cold fixative solution (2.5% glutaraldehyde and 2% PFA (paraformaldehyde) in 0.1 M phosphate buffer). Their eyes were removed, and the retinas were dissected and fragmented in 1 mm³ pieces. Retinal fragments were immersed in fixative solution (2.5% glutaraldehyde and 2% PFA in 0.1 M phosphate buffer) and incubated at 4 °C overnight. The retinal fragments were post-fixed in 1% osmium tetroxide 2% K₄Fe(CN)₆ in the dark for 2 h at 4 °C and rinsed in double-distilled water to remove the osmium. The retinal fragments were dehydrated in ascending concentrations of acetone, then infiltrated, and embedded in Epon (EMS). Blocks were obtained after polymerization at 60 °C for 48 h. Ultrathin sections of 60 nm in thickness were obtained using a UC6 ultramicrotome (Leica Microsystems, Vienna, Austria) and were stained with 2% uranyless and lead citrate. Sections were observed in a Tecnai Spirit 120 Kv TEM (FEI company, Eindhoven, The Netherlands), and images were acquired with a 1 k × 1 k CCD Megaview camera (Olympus Soft Imaging Solutions, Münster, Germany).