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Junb c11

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JunB (C11) is a recombinant protein that corresponds to the C-terminal portion of the human JunB protein. JunB is a transcription factor that is a member of the Jun family and plays a role in regulating gene expression.

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Lab products found in correlation

Colorimetric assay, by Bio-Rad (2 mentions) Batf3 af7437 antibody, by R&D Systems (2 mentions) C jun g4, by Santa Cruz Biotechnology (2 mentions)

2 protocols using junb c11

1

Immunoblotting and Immunoprecipitation of Transcription Factors

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Whole-cell protein lysates were obtained in nondenaturing buffer (150 mmol/L NaCl, 50 mmol/L Tris-pH8, 1% NP-10, 0.25% sodium deoxycholate). Protein concentrations were estimated by Bio-Rad colorimetric assay. Immunoblotting was performed by loading 20μg of protein onto 11% PAGE gels followed by transfer to PVF membranes. Signals were detected by enhanced chemiluminescence (Pierce) or with the Odyssey imaging system. Representative blots are shown. The following primary antibodies used were purchased from Cell Signaling: c-Jun (60A8), P-c-JunSer73 (D47G9), JunB(C37F9), BATF(D7C5), IRF4(4964) and Histone-3(1B1B2). The BATF3 (AF7437) antibody was from R&D. Immunoprecipitations were performed in 100mg of whole-cell protein lysates in 150μL of nondenaturing buffer and 7.5mg of agar-conjugated antibodies c-Jun (G4) or JunB (C11) (Santa Cruz Biotechnology). After overnight incubation at 4°C. Beads were washed 3 times with nondenaturing buffer, and proteins were eluted in Laemmli sample buffer, boiled, and loaded onto PAGE gels. Detection of immunoprecipitated proteins was performed with above-mentioned reagents and antibodies.
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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2

Immunoblotting and Immunoprecipitation of Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole-cell protein lysates were obtained in nondenaturing buffer (150 mmol/L NaCl, 50 mmol/L Tris-pH8, 1% NP-10, 0.25% sodium deoxycholate). Protein concentrations were estimated by Bio-Rad colorimetric assay. Immunoblotting was performed by loading 20μg of protein onto 11% PAGE gels followed by transfer to PVF membranes. Signals were detected by enhanced chemiluminescence (Pierce) or with the Odyssey imaging system. Representative blots are shown. The following primary antibodies used were purchased from Cell Signaling: c-Jun (60A8), P-c-JunSer73 (D47G9), JunB(C37F9), BATF(D7C5), IRF4(4964) and Histone-3(1B1B2). The BATF3 (AF7437) antibody was from R&D. Immunoprecipitations were performed in 100mg of whole-cell protein lysates in 150μL of nondenaturing buffer and 7.5mg of agar-conjugated antibodies c-Jun (G4) or JunB (C11) (Santa Cruz Biotechnology). After overnight incubation at 4°C. Beads were washed 3 times with nondenaturing buffer, and proteins were eluted in Laemmli sample buffer, boiled, and loaded onto PAGE gels. Detection of immunoprecipitated proteins was performed with above-mentioned reagents and antibodies.
Lynn R.C., Weber E.W., Sotillo E., Gennert D., Xu P., Good Z., Anbunathan H., Lattin J., Jones R., Tieu V., Nagaraja S., Granja J., DeBourcy C., Majzner R., Satpathy A.T., Quake S.R., Monje M., Chang H, & Mackall C.L. (2019). c-Jun overexpression in CAR T cells induces exhaustion resistance. Nature, 576(7786), 293-300.
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