Male wistar kyoto wky rats

Male Wistar-Kyoto (WKY) rats weighing 180–200 g (Charles River, Wilmington, MA, USA) were used. Rats were assigned into groups (n = 6 in each group). Treatments were given i.p. in an osmotic pump (2ML, Alzet, Cupertino, CA, USA) starting two days prior to cisplatin injection and included vehicle (20% DMSO in PEG-400 v/v), EET analog A (EET-A in double distilled water, 10 mg/kg/d), or EET-F01 at 2 mg/kg/d and 20 mg/kg/d doses (prepared in a mixture of 20% DMSO in PEG400), respectively. The control-vehicle rat group received drinking water ad libitum and were administered the same DMSO (300–500 µL i.p.) that was used to prepare cisplatin solutions. Rats in the treatment groups were administered a single dose of cisplatin (7 mg/kg i.p.). One day prior to euthanasia, rat urine was collected over a 24 h period. Five days after cisplatin or vehicle administration, rats were anesthetized for blood and kidney sample collection followed by euthanasia.

All experiments were conducted according to the Health Guide for the Use and Care of Laboratory Animals of Shanghai Jiao Tong University. Male Wistar rats (3 weeks old) and male Wistar Kyoto (WKY) rats (3 weeks old) were purchased from Vital River Laboratory Animal Technology Co., Ltd., in Beijing. All rats were maintained on a 12-hr light-dark cycle with lights on at 12 a.m. and lights off at 12 p.m., at room temperature 22 ± 2°C, and at room humidity around 40%. Each rat was housed in a separate cage. WKY rats are required to be housed separately to induce the depression-like behavior; in order to reduce the effect caused by housing conditions, we housed Wistar rats separately as well. After one-week adaptation, rats (4 weeks old) were randomly divided into four groups: Wistar control, Wistar predator stress, WKY control, and WKY predator stress groups; each group consisted of 5 rats. The mean body weights of each group were 113.9 ± 2.3 g, 118.9 ± 4.6 g, 98.2 ± 2.5 g, and 96.8 ± 3.6 g, respectively. All the animals were treated alike except for the predator exposure between the control and stress groups. During the experiment, we recorded the body weight of each rat, both before and after predator stress. The percentage of weight change was calculated as (weight after predator stress—weight before predator stress)/weight before predator stress ∗ 100%.