2 buoh

ITN working compound was provided by BOC Sciences (Shirley, NY, USA). MeOH, EtOH, 1-BuOH, 2-BuOH, EG, PG, and PEG-400 were procured from Fisher Chemical (Loughborough, UK). EA and DMSO were acquired from Sigma Aldrich (Darmstadt, Germany). THP was obtained from Gattefosse (Lyon, France). The Milli-Q unit was used to obtain ultra-pure water. Supplementary Table S1 provides an overview of each material’s specifics.

Solvents used for the sample preparation and HPLC-FLD analysis were all HPLC-grade, while solvents used for the HPLC-HRMS analysis were MS-grade. Acetonitrile (ACN), ammonium formate, formic acid, ethyl acetate, MeOH, and trifluoroacetic acid were all purchased from Sigma-Aldrich (Schnelldorf, Germany), while 2-BuOH was purchased from Fisher Scientific (Waltham, MA, USA). Water was purified by reverse osmosis and a subsequent MilliQ-system (Millipore, Bedford, MA, USA). Fluorescence derivatization was achieved using AccQ-Fluor reagent WAT052880 (Waters, Milford, MA, USA). External calibration was carried out using a fumonisin B₁ (49.9 µg/mL) and fumonisin B₂ (50.6 µg/mL) mixture in H₂O:ACN (1:1, v/v), purchased from Romer Labs (Tulln, Austria). Four different Isolute SPE cartridges (C18 (MFC) (3 mL, 500 mg), C8 (3 mL, 500 mg), C2 (3 mL, 500 mg) and Isolute Myco (3 mL, 60 mg)) were purchased from Biotage (Uppsala, Sweden), while Strata-X (3 mL, 30 mg) SPE cartridges were purchased from Phenomenex (Torrance, CA, USA).