Combined glass electrode

Manufactured by Metrohm

Sourced in Germany

The combined glass electrode is a type of pH electrode used for measuring the pH value of a solution. It consists of a pH-sensitive glass membrane and a reference electrode integrated into a single housing. The glass membrane generates a potential difference that is proportional to the hydrogen ion (H+) concentration in the solution, allowing for accurate pH measurements.

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Lab products found in correlation

765 dosimat burette, by Metrohm (1 mentions) Quartz cuvette, by Hellma (1 mentions) Sz 100 nanoparticle analyzer, by Horiba (1 mentions) 827 ph meter, by Metrohm (1 mentions) Carry 60 uv vis spectrophotometer, by Agilent Technologies (1 mentions)

3 protocols using combined glass electrode

Spectrophotometric Determination of Ochratoxin A

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The absorption spectra of OTA solutions were acquired in the range 250–450 nm with a Jasco V-550 UV-Vis double-beam spectrophotometer, provided with Hellma quartz cuvettes for flow-through measurement (10.00 mm optical path length). The pH of the solutions was evaluated by a Metrohm potentiometer (model 713, resolution of 0.001 pH units) equipped with a Metrohm combined glass electrode (mod. 6.0259.100). The combined electrode was daily calibrated in terms of H⁺ concentration. The slope and the formal potential E⁰ of the Nernst equation were elaborated by the ESAB2M program.³³ The experiments were conducted in oxygen-free atmosphere by flowing nitrogen in the solution, in continuous stirring, at 25 °C (thermocryostat mod. D1-G Haake). Initially, the OTA solution (20 mL) was adjusted to pH 3 with HCl. Subsequent aliquots of NaOH solution (10 μL) were added by a Metrohm 765 Dosimat burette (resolution 1 μL) until the OTA solution reached a pH value of about 7.5. Each addition corresponded to an increase of 0.1/0.2 pH units.
A total of 45 UV-Vis spectra were elaborated with HypSpec® software^{34 (link)} that operates with stoichiometric principles by applying mass and charge balance equations and Lambert–Beer's law.

Cagnasso I., Tonachini G., Berto S., Giacomino A., Mandrile L., Maranzana A, & Durbiano F. (2019). Comprehensive study on the degradation of ochratoxin A in water by spectroscopic techniques and DFT calculations. RSC Advances, 9(34), 19844-19854.

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Physicochemical Characterization of Silver Nanoparticles

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The UV–Vis absorbance measurements were done using a Carry 60 UV–vis spectrophotometer (Agilent, USA) by a quartz cuvette (10 mm). The X-ray diffraction (XRD) were performed by the XRD Bruker D8 Advance powder diffractometer (Bruker, Germany) by applying the reflection mode with Cu-K_α radiation (λ = 1.5406 Å). The Fourier Transform-Infra Red (FT-IR) spectra were collected at room temperature using a “Spectrum RXI” Perkin-Elmer FT-IR spectrophotometer (PerkinElmer, USA) after tableting the AgNPs powder. The distribution of size and volume of NPs were also determined using Dynamic Light Scattering (DLS) using HORIBA SZ-100 nanoparticle analyzer (HORIBA, Japan).
The morphology of the particles was investigated using the Transmission Electron Microscopic examination (TEM). For this study, colloidal AgNPs were sonicated and a thin film was formed on the carbon coated grid Cu Mesh 300. TEM measurements were done using a Zeiss–EM10C microscope (Zeiss, Germany) operated at an accelerating voltage of 80 kV. The pH adjustment during synthesis was performed using a Metrohm 827 pH-meter equipped with a combined glass electrode (Metrohm, Switzer-land).

Nikaeen G., Yousefinejad S., Rahmdel S., Samari F, & Mahdavinia S. (2020). Central Composite Design for Optimizing the Biosynthesis of Silver Nanoparticles using Plantago major Extract and Investigating Antibacterial, Antifungal and Antioxidant Activity. Scientific Reports, 10, 9642.

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Potentiometric Titration of Amine-NG and MA-NG

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To determine the amount of incorporated amine comonomer within the Amine-NG, and the degree of functionalization of MA-NG, a potentiometric titration method was used to determine the number of primary amine groups [33 (link)]. The potentiometric titrations were conducted on a Metrohm 702 SM Titrino titrator at room temperature. A representative procedure can be described as follows: approximately 20 mg of Amine-NG or MA-NG was dispersed in 50 mL of water and transferred into the titration cell. The pH was adjusted to approximately 10 with 0.1 M NaOH. After an equilibration time of 15 min, the titration was performed by the addition of 0.1 M HCl in increments of 2 µL, and the pH was simultaneously measured with a Metrohm combined glass electrode. The amount of incorporated amine was calculated from the dependence of the pH on the volume of the added titrant. At least three independent experiments were performed for each sample tested.

Zu G., Meijer M., Mergel O., Zhang H, & van Rijn P. (2021). 3D-Printable Hierarchical Nanogel-GelMA Composite Hydrogel System. Polymers, 13(15), 2508.

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