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Vectra polaris platform

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The Vectra Polaris platform is a multispectral imaging system designed for advanced tissue analysis. It enables high-resolution imaging and quantitative analysis of multiple biomarkers within a single tissue sample.

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Lab products found in correlation

Inform software, by PerkinElmer (3 mentions) Scn400f platform, by Leica (1 mentions)

3 protocols using vectra polaris platform

1

Quantification of Micronuclei in Ovarian Tumors

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High-grade ovarian tumor samples were obtained from MD Anderson Tissue Bank under an approved institutional review board protocol, and written consent was obtained for the use of patient samples for research. The details regarding the quality control for the samples obtained from CHTN can be found at https://www.chtn.org/quality.html. For MN identification and quantification, the Vectra Polaris platform (PerkinElmer) was used, and inForm software (PerkinElmer) was used to systematically count MN. After tissue segmentation, a number of nuclei in views were calculated by the cell segmentation process, and MN was defined as over 0.85 roundness and less than approximately 3 μm size (95 pixels in 96 dpi images), as shown in Fig. 3D.
Yokoi A., Villar-Prados A., Oliphint P.A., Zhang J., Song X., De Hoff P., Morey R., Liu J., Roszik J., Clise-Dwyer K., Burks J.K., O’Halloran T.J., Laurent L.C, & Sood A.K. (2019). Mechanisms of nuclear content loading to exosomes. Science Advances, 5(11), eaax8849.
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2

Multiplex Digital Pathology Analysis of Tumor Microenvironment

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Digital images were captured with the PerkinElmer Vectra-Polaris platform following hot spot lymphocyte assessment: Areas in the tumor-stroma interface were scanned at ×20 and selected for analysis. We obtained six images of 0.36 mm2 each per tissue sample for analysis. Multiplexed images were analyzed with InForm Software (PerkinElmer). The total number of cells per mm2 was enumerated for all the cell phenotypes expressed in the stroma and the tumor compartment. Tissue samples stained by conventional H&E were scanned with the Leica SCN400F platform at ×20 and magnified at ×200–400 for immune infiltrate evaluation.
Duhen R., Ballesteros-Merino C., Frye A.K., Tran E., Rajamanickam V., Chang S.C., Koguchi Y., Bifulco C.B., Bernard B., Leidner R.S., Curti B.D., Fox B.A., Urba W.J., Bell R.B, & Weinberg A.D. (2021). Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells. Nature Communications, 12, 1047.
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3

Spatial Profiling of Immune Cells in Cancer

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The frequency and localization of immune cell subpopulations and cancer cells from 374 samples was determined by MSI and is representatively shown in Figure 1. The staining procedure was performed as recently described (30 (link)). The Ab panel included CD3, CD8, FOXP3, CD20, CD163 and panCK (Supplementary Table S1). For imaging the PerkinElmer Vectra Polaris platform was employed. Cell segmentation and phenotyping were performed using the inForm software (PerkinElmer Inc., USA, Version 2.4). The frequency and spatial distribution of cell populations were analysed using an R script (https://github.com/akoyabio).
Ullu E, & Tschudi C. (1991). Trans splicing in trypanosomes requires methylation of the 5' end of the spliced leader RNA.. Proceedings of the National Academy of Sciences of the United States of America, 88(22), 10074-10078.
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