Losartan

Manufactured by Fujifilm

Sourced in Japan

Losartan is a laboratory reagent used in various analytical and research applications. It functions as an angiotensin II receptor antagonist, which plays a role in regulating blood pressure and fluid balance. The core function of Losartan is to serve as a research tool for scientists and researchers working in fields such as biochemistry, pharmacology, and medical research.

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Lab products found in correlation

6 protocols using losartan

Pharmacological Modulation of Angiotensin and Calcium Signaling

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Angiotensin II (Peptide Institute, Osaka, Japan), losartan (Wako Pure Chemical Industries, Osaka, Japan), PD123,319 (Sigma-Aldrich, St. Louis, MO, USA), angiotensin I (Peptide Institute, Osaka, Japan), captopril (Sigma-Aldrich, St. Louis, MO, USA), chymostatin (Sigma-Aldrich, St. Louis, MO, USA) and nickel(II) chloride hexahydrate (Sigma-Aldrich, St. Louis, MO, USA) were dissolved in water. Carvedilol (Tokyo Chemical Industry, Tokyo, Japan), xestospongin C (Wako Pure Chemical Industries, Osaka, Japan), SEA0400 (synthesized in our faculty according to the reported method [75 (link)]), 2-APB (Sigma-Aldrich, St. Louis, MO, USA) and ryanodine (Wako Pure Chemical Industries, Osaka, Japan) were dissolved in dimethyl sulfoxide (DMSO). They were added to the organ bath to obtain the desired final concentration.

Tanaka Y., Obata K., Ohmori T., Ishiwata K., Abe M., Hamaguchi S., Namekata I, & Tanaka H. (2019). Angiotensin II Induces Automatic Activity of the Isolated Guinea Pig Pulmonary Vein Myocardium through Activation of the IP3 Receptor and the Na+-Ca2+ Exchanger. International Journal of Molecular Sciences, 20(7), 1768.

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Striatal Intracerebral Perfusion of Receptor Antagonists

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Losartan, lisinopril and AngII were purchased from Wako Pure Chemical (Tokyo, Japan). Benazepril was purchase from Tokyo Chemical Industry (Tokyo, Japan). PD123319, ZD7155, A779, SR202 and EHT1864 were purchased from Tocris Bioscience (Bristol, UK). Diphenylene iodium (DPI) and 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF) were purchased from Sigma-Aldrich. A water-soluble derivative of forskolin, 7-deacetyl-7-[O-(N-methylpiperazino)-γ-butyryl]-forskolin (forskolin-ws), was purchased from Calbiochem (San Diego, CA, USA).
Antagonists of AT1R (losartan and ZD7155) and AT2R (PD123319), the Mas receptor (A779) and PPARγ (SR202) and inhibitors of ACE (benazepril and lisinopril), NOX (DPI and AEBSF) and Rac (EHT1864) were dissolved in the perfused solution and administered to the striatum through the probe during the experimental period. Forskolin-ws, which stimulates cAMP production by activating adenylyl cyclase^{48 (link)}, and AngII were dissolved in sterilized physiological saline (Otsuka Pharmaceutical, Tokyo, Japan) and directly administered into the striatum through the thin needle of the MI-A-I-8-03 probe using an ESP-32 pump. The flow rate was 0.1 μL/min, and the total volume was 1 μL for forskolin-ws and 2 μL for AngII.

Hara S., Kobayashi M., Kuriiwa F., Mizukami H, & Mukai T. (2020). Blockade of the renin-angiotensin system suppresses hydroxyl radical production in the rat striatum during carbon monoxide poisoning. Scientific Reports, 10, 2602.

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Preparation and Storage of Angiotensin II Receptor Antagonists

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Telmisartan and valsartan were purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). Losartan and irbesartan were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). We prepared solutions of Telmisartan, valsartan and irbesartan by diluting with dimethyl sulfoxide (DMSO). Losartan was prepared by diluting with H₂O. The stock solutions were stored at −20°C.

Oura K., Tadokoro T., Fujihara S., Morishita A., Chiyo T., Samukawa E., Yamana Y., Fujita K., Sakamoto T., Nomura T., Yoneyama H., Kobara H., Mori H., Iwama H., Okano K., Suzuki Y, & Masaki T. (2017). Telmisartan inhibits hepatocellular carcinoma cell proliferation in vitro by inducing cell cycle arrest. Oncology Reports, 38(5), 2825-2835.

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Unilateral Ureteral Obstruction in Mice

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Female C57BL/6J mice (9 weeks of age) were performed unilateral ureteral obstruction (UUO) surgery. Under isoflurane anesthesia, the left ureter was occluded by two-point ligations and cut between them. After 1 or 7 days of surgery, mice were euthanized by exsanguination under anesthesia with isoflurane. Then, the obstructed kidney was harvested for analysis of CYP4A and GPR75 protein expression. Age-matched female C57BL/6J mice served as normal controls. In the intervention study, we randomly assigned the mice to following treatment groups: vehicle (0.5% MC), TP0472993 (0.3 and 3 mg/kg), and Losartan (n=8 / group). The mice were administered 0.5% MC or TP0472993 (suspension in 0.5% MC) via oral gavage twice a day starting the day of operation and for 7 days after the surgery. Losartan (0.1 mg/mL) (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was administered via drinking water (Song et al., 2019; Ougaard et al., 2018) .
Age-matched, vehicle-treated female C57BL/6J mice served as normal controls. After 7 days of the surgery, all animals were killed by exsanguination under anesthesia with isoflurane, and the obstructed kidney was harvested for histopathological and biochemical analysis.

Hirata T., Ohara H., Kojima N., Koretsune H., Hasegawa Y., Inatani S, & Takahashi T. (2023). Renoprotective Effect of TP0472993, a Novel and Selective 20-Hydroxyeicosatetraenoic Acid Synthesis Inhibitor, in Mouse Models of Renal Fibrosis. The Journal of pharmacology and experimental therapeutics, 386(1).

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Angiotensin II Receptor Antagonist Preparation

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Telmisartan and valsartan were purchased from Tokyo Chemical Industry Co. (Tokyo, Japan). Irbesartan and losartan were purchased from Wako Pure Chemical Industries (Osaka, Japan). Telmisartan was prepared as a 10 mM stock solution in dimethyl sulfoxide (DMSO). Irbesartan and valsartan were prepared as 100 mM stock solutions in DMSO, and losartan was prepared as a 100 mM stock solution in H₂O. The stock solutions were stored at -20°C. Compound C was purchased from Abcam (Cambridge, UK). MK-2206 was purchased from ChemScene Chemicals (Monmouth Junction, NJ, USA). SCH772984 was purchased from Selleck Chemicals (Houston, TX, USA).

Fujihara S., Morishita A., Ogawa K., Tadokoro T., Chiyo T., Kato K., Kobara H., Mori H., Iwama H, & Masaki T. (2016). The angiotensin II type 1 receptor antagonist telmisartan inhibits cell proliferation and tumor growth of esophageal adenocarcinoma via the AMPKa/mTOR pathway in vitro and in vivo. Oncotarget, 8(5), 8536-8549.

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Mitochondrial Dynamics and Signaling Pathways

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Amlodipine was from Tokyo Chemical Industry Co. Ltd. Mdivi-1, sildenafil, atenolol, propranolol, metoprolol, thiocarbohydrazide solution, durcupan resin, c-Myc peptide, and GTP-agarose were from Sigma-Aldrich. Dulbecco's modified Eagle's medium (DMEM), diltiazem, nifedipine, nisoldipine, nicardipine, nitrendipine, verapamil, losartan, uranium acetate, anti-Myc, and anti--actin were from Wako. MitoTracker Green FM, MitoTracker Red CM-H 2 XRos, and MitoSox Red were from Invitrogen. Collagenase II was from Worthington. Osmium tetroxide was from Electron Microscopy Science. Bovine serum albumin (BSA), sodium cacodylate, and phenol red-free DMEM were from Nacalai Tesque. Anti-Drp1 (H-300), anti-GAPDH (FL-335), anti-SOD2 (Mn-SOD: N-20), anti-Mfn1 (H-65), and anti-filamin 1 (FLNa: H-300 and E-3) were from Santa Cruz Biotechnology. Anti-p53 (7F5) and horseradish peroxidase (HRP)-conjugated anti-mouse and rabbit IgG were from Cell Signaling Technology. Anti-tafazzin, anti-HIF-1, anti-sarcomeric -actinin (EA-53), and anti-Mfn2 were from Abcam. Anti-Opa1 was from BD Biosciences. Anti-HO-1 was from Enzo Life Sciences. EGTA and Hepes were from Dojindo.

Nishimura A., Shimauchi T., Tanaka T., Shimoda K., Toyama T., Kitajima N., Ishikawa T., Shindo N., Numaga-Tomita T., Yasuda S., Sato Y., Kuwahara K., Kumagai Y., Akaike T., Ide T., Ojida A., Mori Y, & Nishida M. (2018). Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission-associated myocardial senescence. Science signaling, 11(556).

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