The aforementioned fixed and embedded heart samples were cut into 4-µm sections and then deparaffinized using xylene. The antigen retrieval was performed by heating the tissues to 80°C. Subsequently, the sections were incubated with Protease K for 15 min at room temperature before pre-incubation with terminal deoxynucleotidyl transferase buffer (Sigma-Aldrich; Merck KGaA) at 37°C for 60 min. After washing with PBS, the sections were incubated with an anti-digoxin and anti-serum alkaline phosphatase complex (Sigma-Aldrich; Merck KGaA) at 37°C overnight. Following washing in Tris buffer, sections were counterstained with hematoxylin at 25°C for 2 min and washed again in Tris buffer. In total, five visual fields were randomly selected in each group to observe the apoptosis under a light microscope (BX53; Olympus Corporation; magnification, ×200). The results were quantified using Image-Pro Plus software (version 6.0; Media Cybernetics, Inc.).
Anti digoxin and anti serum alkaline phosphatase complex
Manufactured by Merck Group
Sourced in United States
The anti-digoxin and anti-serum alkaline phosphatase complex is a laboratory equipment product designed to detect and measure the presence of digoxin, a cardiac glycoside, in biological samples. This complex utilizes antibodies specific to digoxin and alkaline phosphatase, an enzyme, to facilitate the analysis. The core function of this product is to provide a reliable and accurate method for the quantification of digoxin levels in various clinical and research settings.
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Lab products found in correlation
2 protocols using anti digoxin and anti serum alkaline phosphatase complex
1
Apoptosis Assessment in Cardiac Tissue
2
Quantifying Hippocampal Apoptosis in Rats
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Brain sections were fixed in 4% neutral PFA and taken out for paraffin embedding. Then, sections were dewaxed in xylene and hydrated in ethanol in gradient concentrations. Following rinsing sections in tap water, antigen retrieval was implemented at 80°C. Thereafter, the sections were incubated in presence of Protease K (Sigma, U.S.A.) and then placed in TDT buffer (Sigma, U.S.A.) for pre-incubation. After washed with PBS again, the sections were then incubated with anti-digoxin and anti-serum alkaline phosphatase complex (Sigma, U.S.A.) at 37°C overnight. Following washes in Tris-buffer, counter staining was performed with the corresponding reagent, and ended using Tris-buffer. Apoptosis in hippocampus of rats was observed under a microscope, and the results were quantified in Image-Pro Plus. Apoptotic ratio is calculated using the formula: Apoptotic ratio = TUNEL-positive cell quantity/total quantity of cells. The experiment was repeated three times.
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