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Midazolam

Manufactured by Cayman Chemical
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Midazolam is a benzodiazepine compound used as a laboratory reagent. It functions as a sedative and hypnotic agent. The product is intended for research purposes only and should not be used for any other application.

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Lab products found in correlation

Mitragynine, by Cayman Chemical (4 mentions) 7 hydroxymitragynine, by Cayman Chemical (4 mentions) Mitragynine d3, by Cayman Chemical (3 mentions) Methanol, by Thermo Fisher Scientific (3 mentions) Acetonitrile, by Thermo Fisher Scientific (3 mentions) 1 hydroxymidazolam, by Cayman Chemical (3 mentions) Dextromethorphan, by Cayman Chemical (2 mentions) Tert butyl methyl ether, by Merck Group (2 mentions) Dextrorphan, by Cayman Chemical (2 mentions) Dextromethorphan d3, by Cayman Chemical (2 mentions) 4 hydroxymidazolam, by Cayman Chemical (2 mentions) Midazolam d4, by Cayman Chemical (2 mentions) Bupropion hydrochloride, by Merck Group (2 mentions) Chlorzoxazone, by Cayman Chemical (2 mentions) Dextrorphan d3, by Merck Group (2 mentions) Acetaminophen d4, by Cayman Chemical (2 mentions) Hydroxybupropion d6, by Cambridge Isotopes (2 mentions) 7 hydroxycoumarin d5 sulfate, by LGC (2 mentions) 7 hydroxycoumarin 13c6 glucuronide, by LGC (2 mentions) Hydroxymidazolam 13c3, by Corning (2 mentions)

Spelling variants of this product

Midazolam-d4 (2 citations)

9 protocols using midazolam

1

Quantitative Analysis of Kratom Alkaloids

Cited 1 time
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Chloroquine, 7-hydroxymitragynine, midazolam, mitragynine, and mitragynine-d3 were purchased from Cayman Chemical (Ann Arbor, MI, USA). The kratom alkaloids speciogynine, mitraciliatine, speciociliatine, paynantheine, and isopaynantheine were isolated and purified from Mitragyna speciosa as detailed previously [7 (link)]; all standards were >95% pure as measured by ultraperformance liquid chromatography (UPLC). Alprazolam and tert-butyl methyl ether (t-BME) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Human liver microsomes (HLMs; H0604, mixed biological sex, pool of 15, lot 1010191) and human intestinal microsomes (HIMs; H0610.I, mixed biological sex, pool of 10, lot 1610314) were purchased from XenoTech, LLC (Kansas City, KS, USA). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Blank human plasma (K2EDTA, mixed biological sex, pooled, lot HMN99236) and whole blood (K2EDTA, mixed biological sex, pooled, lot HMN541526) were purchased from BioIVT (Westbury, NY, USA). All other chemicals and reagents were analytical grade.
Tanna R.S., Nguyen J.T., Hadi D.L., Manwill P.K., Flores-Bocanegra L., Layton M.E., White J.R., Cech N.B., Oberlies N.H., Rettie A.E., Thummel K.E, & Paine M.F. (2022). Clinical Pharmacokinetic Assessment of Kratom (Mitragyna speciosa), a Botanical Product with Opioid-like Effects, in Healthy Adult Participants. Pharmaceutics, 14(3), 620.
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2

Quantification of Kratom Alkaloids in Plasma

Cited 2 times
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Dextromethorphan, Dextromethorphan-d3, dextrorphan, 1′-hydroxymidazolam, 4-hydroxymidazolam, 7-hydroxymitragynine, midazolam, midazolam-d4, mitragynine, and mitragynine-d3 were purchased from Cayman Chemical (Ann Arbor, MI). The kratom indole alkaloids speciociliatine, speciogynine, mitraciliatine, paynantheine, and isopaynantheine were isolated and purified from commercial kratom products as described3 (link),5 (link); all reference standards were verified to be ≥95% pure as measured by ultra-high-performance liquid chromatography-ultraviolet spectroscopy (UHPLC-UV) analysis. tert-Butyl methyl ether was purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ). Blank human plasma (K2EDTA, pooled, mixed gender, lot HMN99236) was purchased from BioIVT (Westbury, NY). All other chemicals and reagents were analytical grade.
Naiman B.M., Alt D., Bolin C.A., Zuerner R, & Baldwin C.L. (2001). Protective Killed Leptospira borgpetersenii Vaccine Induces Potent Th1 Immunity Comprising Responses by CD4 and γδ T Lymphocytes. Infection and Immunity, 69(12), 7550-7558.
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3

Quantification of Kratom Alkaloids in Plasma

Cited 2 times
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Dextromethorphan, Dextromethorphan-d3, dextrorphan, 1′-hydroxymidazolam, 4-hydroxymidazolam, 7-hydroxymitragynine, midazolam, midazolam-d4, mitragynine, and mitragynine-d3 were purchased from Cayman Chemical (Ann Arbor, MI). The kratom indole alkaloids speciociliatine, speciogynine, mitraciliatine, paynantheine, and isopaynantheine were isolated and purified from commercial kratom products as described3 (link),5 (link); all reference standards were verified to be ≥95% pure as measured by ultra-high-performance liquid chromatography-ultraviolet spectroscopy (UHPLC-UV) analysis. tert-Butyl methyl ether was purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ). Blank human plasma (K2EDTA, pooled, mixed gender, lot HMN99236) was purchased from BioIVT (Westbury, NY). All other chemicals and reagents were analytical grade.
Tanna R.S., Nguyen J.T., Hadi D.L., Layton M.E., White J.R., Cech N.B., Oberlies N.H., Rettie A.E., Thummel K.E, & Paine M.F. (2023). Clinical Assessment of the Drug Interaction Potential of the Psychotropic Natural Product Kratom. Clinical pharmacology and therapeutics, 113(6), 1315-1325.
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4

Analytical Reagents for Drug Metabolism

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All chemicals and reagents were used as received unless otherwise specified. Chlorzoxazone, dextromethorphan (hydrobromide hydrate), (S)-mephenytoin, midazolam, testosterone, and acetaminophen-d4 were purchased from Cayman Chemical Company. Collagen I (rat tail) was purchased from Enzo Life Sciences. Phenacetin, dextrorphan-d3, bupropion hydrochloride, and sodium hydroxide (NaOH) were purchased from Millipore Sigma. Dimethyl sulfoxide (DMSO) and 7-hydroxycoumarin were purchased from Fisher Scientific. Hydroxybupropion-d6 was purchased from Cambridge Isotope Laboratories. 6-hydroxyChlorzoxazone-d2 was purchased from Clearsynth. 7-hydroxycoumarin-d5-sulfate and 7-hydroxycoumarin-13C6-glucuronide were purchased from Toronto Research Chemicals. 4-hydroxymephenytoin-d3 was purchased from MuseChem. Hydroxymidazolam-13C3 and hydroxytestosterone-d7 were purchased from Corning.
Diprospero T.J., Brown L.G., Fachko T.D, & Lockett M.R. (2023). HepaRG cells undergo increased levels of post-differentiation patterning in physiologic conditions when maintained as 3D cultures in paper-based scaffolds. bioRxiv.
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5

Cytochrome P450 Enzyme Inhibition Assay

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DAMGO, quinidine, ketoconazole, morphine sulfate, and alprazolam were purchased from MilliporeSigma (St Louis, MO). Mitragynine, 7-hydroxyMitragynine, midazolam, 1′-hydroxymidazolam, sulfaphenazole, and NADPH were obtained from Cayman Chemical Company (Ann Arbor, MI). Diclofenac, 4′-hydroxyDiclofenac, dextromethorphan, and dextrorphan were obtained from Toronto Research Chemicals, Inc. (Toronto, Canada). Potassium phosphate buffer salts were obtained from Fisher Scientific (Fair Lawn, NJ). Human liver microsomes were obtained from XenoTech, LLC (H0604, mixed gender, pool from 15 donors, lot no. 1010191; Kansas City, KS). All other chemicals and reagents used were analytical grade.
Todd D.A., Kellogg J.J., Wallace E.D., Khin M., Flores-Bocanegra L., Tanna R.S., McIntosh S., Raja H.A., Graf T.N., Hemby S.E., Paine M.F., Oberlies N.H, & Cech N.B. (2020). Chemical composition and biological effects of kratom (Mitragyna speciosa): In vitro studies with implications for efficacy and drug interactions. Scientific Reports, 10, 19158.
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6

Quantification of Steroid and Drug Compounds

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Androstenedione (9001311), corticosterone (16063), medroxyprogesterone (24908), progesterone (15876), testosterone (15645), gefitinib (13166), schisandrin A (19849), α-naphthoflavone (16924), and midazolam (16193) were purchased from Cayman Chemical Company. POPC lipids were purchased from Avanti Polar Lipids. All other chemicals were purchased from Sigma-Aldrich. Structures are shown in the Scheme 1.
Denisov I.G., Grinkova Y.V., McLean M.A., Camp T, & Sligar S.G. (2022). Midazolam as a Probe for Heterotropic Drug-Drug Interactions Mediated by CYP3A4. Biomolecules, 12(6), 853.
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7

Quantitative analysis of ketamine and its metabolites

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Actoacetanilide was the product of the Tokyo Chemical Industry (Tokyo, Japan). S-Ketamine was obtained from Cayman Chemical Company (Ann Arbor, MI, USA) as a certified reference material (CRM) solution in methanol (1 mg/mL). Before using this reagent, the methanol solvent was evaporated under a stream of argon gas, and the chemical was redissolved in 0.1 M Hepes buffer pH 7.4 containing 60 mM KCl. The concentration of ketamine in the stock solution was determined from its absorbance at 265 nm using the extinction coefficient of 0.562 mM−1cm−1. (S)-norketamine hydrochloride and midazolam were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Glucose-6-phosphate dehydrogenase from Leuconostoc mesenteroides, NADP, Glucose-6-phosphate, and midazolam were the products of MilliporeSigma (Burlington, MA, USA). All other reagents were of ACS grade and used without additional purification.
Davydova N.Y., Hutner D.A., Gaither K.A., Singh D.K., Prasad B, & Davydov D.R. (2023). High-Throughput Assay of Cytochrome P450-Dependent Drug Demethylation Reactions and Its Use to Re-Evaluate the Pathways of Ketamine Metabolism. Biology, 12(8), 1055.
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8

Generating TrkA-KFG Mutant Mice for Behavioral Studies

Cited 1 time
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The generation of TrkA-KFG mutant mice has been described elsewhere [20 (link)]. All tests were conducted on at least 3-month-old, age-matched, male mutants, or C57BL/6CR mice. Male mice were used to exclude the effects of sexual dimorphism and estrous phase on behavior. Animals were kept in the NCI vivarium in colony cages at an ambient temperature of 25 ± 2 °C and 45–55% relative humidity with 12 h light: dark cycle. The mice had free access to standard rodent pellet diet and drinking water. Donepezil (3 mg/kg i.p) was obtained from Sigma-Aldrich (USA). Picrotoxin (0.5 mg/kg i.p) and midazolam (1.25 mg/g i.p) were from Cayman Chemicals (USA). The drug dosage was based on results of pilot experiments and earlier publications [21 (link)–23 (link)]. TMT (2, 3, 5-Trimethyl-3-thiazoline) was obtained from PheroTech, Canada. All procedures were approved by NCI-Frederick ACUC committee and followed the National Institutes of Health Guidelines for animal care and use.
Yanpallewar S., Tomassoni-Ardori F., Palko M.E., Hong Z., Kiris E., Becker J., Fulgenzi G, & Tessarollo L. (2022). TrkA-cholinergic signaling modulates fear encoding and extinction learning in PTSD-like behavior. Translational Psychiatry, 12, 111.
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9

Analytical Reagents for Drug Metabolism

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All chemicals and reagents were used as received unless otherwise specified. Chlorzoxazone, dextromethorphan (hydrobromide hydrate), (S)-mephenytoin, midazolam, testosterone, and acetaminophen-d4 were purchased from Cayman Chemical Company. Collagen I (rat tail) was purchased from Enzo Life Sciences. Phenacetin, dextrorphan-d3, bupropion hydrochloride, and sodium hydroxide (NaOH) were purchased from Millipore Sigma. Dimethyl sulfoxide (DMSO) and 7-hydroxycoumarin were purchased from Fisher Scientific. Hydroxybupropion-d6 was purchased from Cambridge Isotope Laboratories. 6-hydroxyChlorzoxazone-d2 was purchased from Clearsynth. 7-hydroxycoumarin-d5-sulfate and 7-hydroxycoumarin-13C6-glucuronide were purchased from Toronto Research Chemicals. 4-hydroxymephenytoin-d3 was purchased from MuseChem. Hydroxymidazolam-13C3 and hydroxytestosterone-d7 were purchased from Corning.
Diprospero T.J., Brown L.G., Fachko T.D, & Lockett M.R. (2023). HepaRG cells undergo increased levels of post-differentiation patterning in physiologic conditions when maintained as 3D cultures in paper-based scaffolds. Research Square.
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