Alfa Aesar Ti foils with a purity of more than 99.5 % and with a thickness of 0.25 mm, tetrachloroauric acid (H[AuCl4] 99.9+%) as a precursor, 99.0 % isopropanol as a sacrificial donor, perchloric acid HClO4 70 % From MERCK, Made in Germany, TiO2 nanoparticle powder prepared in our lab with a surface area of 109 m2/g, Nafion (5 wt %), H2SO4 from Sigma–Aldrich, Potassium chloride GRG KCl (WINLAB), potassium hexacyanoferrate (II) trihydrate K4[Fe(CN)6], and flakes extra pure KOH from LOBA Chemie, India. Mueller-Hinton agar (MHA, HIMEDIA, India), dimethyl sulfoxide (DMSO), potassium hexacyanoferrate(III) K3[Fe (CN)6] (MERCK), glycerol, and gentamicin (10 μg/ml) were obtained from MERCK.
Potassium hexacyanoferrate 3 k3 fe cn 6
Manufactured by Merck Group
Sourced in United States, Belgium, Germany
Potassium hexacyanoferrate (III) (K3[Fe(CN)6]) is a chemical compound that consists of a central iron atom coordinated to six cyanide ligands. It is a yellow crystalline solid that is soluble in water.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Ammonia solution, by Samchun (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
N n dimethylformamide (dmf), by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Uric acid, by Merck Group (2 mentions)
Perchloric acid hclo4, by Merck Group (1 mentions)
H2so4, by Merck Group (1 mentions)
Nafion, by Merck Group (1 mentions)
Glycerol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Mueller hinton agar (mha), by HiMedia (1 mentions)
Gentamicin, by Merck Group (1 mentions)
Sulfuric acid h2so4, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride, by Merck Group (1 mentions)
Sodium dodecyl sulfonate, by Merck Group (1 mentions)
1 10 phenanthroline, by Merck Group (1 mentions)
Iron 2 chloride hexahydrate, by Merck Group (1 mentions)
24 protocols using potassium hexacyanoferrate 3 k3 fe cn 6
1
Synthesis of TiO2-Au Nanocomposite Electrocatalyst
2
Chitosan-Based Electrochemical Biosensor
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All materials used in this study were obtained in their analytical or biological grades. Deacetylated chitosan (CH, 75%), citric acid (CA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, 98%), N-hydroxysuccinimide (NHS, 98%), potassium hexacyanoferrate(III) (K3[Fe(CN)6]), sulfuric acid (H2SO4, 95.0–98.0%), KCl, were obtained from Merck Company. Milli-Q water was used for the preparation of all reagent solutions. The phosphate buffer solution with a pH over 2.0–8.0 was used as the working buffer solution, as reported34 (link). The DNA probe was designed for the IS900 gene, specifically for MAP. All oligonucleotides were provided by Takapouist Biotech. Co. Ltd. (Iran). All oligonucleotide stock solutions were prepared with 10.0 mM in Milli-Q water (pH: 7.4) and stored at − 20 °C. Notably, the details of the oligonucleotide sequences used in this study are provided in Table s2 .
3
Spectrophotometric Determination of Iron
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CDs were freshly prepared by appropriate amount of solid in deionized water. Iron(iii ) chloride hexahydrate (FeCl3·6H2O), iron(ii ) chloride hexahydrate (FeCl2·6H2O), 1,10-phenanthroline, hydrochloric acid and ethanol were all from Merck (Darmstadt, Germany). For preparation of 1,10-phenanthroline solution, hydrochloric acid (0.5 mol L−1) was used. Deionized water was used throughout this study as solvent.
For Prussian blue assay, gallic acid (3,4,5-trihydroxybenzoic acid monohydrate) was purchased from Sigma-Aldrich (Taufkirchen, Germany). Analytical reagent grade potassium hexacyanoferrate(iii ) (K3[Fe(CN)6]) and sodium dodecyl sulfonate (SDS) were purchased from Merck (Darmstadt, Germany).
For Prussian blue assay, gallic acid (3,4,5-trihydroxybenzoic acid monohydrate) was purchased from Sigma-Aldrich (Taufkirchen, Germany). Analytical reagent grade potassium hexacyanoferrate(
4
Electrochemical Glucose Sensor Fabrication
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Cobalt(II) nitrate hexahydrate (Co(NO 3 ) 2 $6H 2 O) was purchased from Fischer Scientifics. Potassium hexacyanoferrate(III) (K 3 Fe(CN) 6 ) and Potassium chloride (KCl) were purchased from Sigma Aldrich. The sensing reagent D(þ)-glucose was obtained from Aromel Kimya. The interference reagents D(þ)-gluconolactone, L- ascorbic acid, uric acid, and D(þ)-lactose were purchased from Acros Organics. Sucrose was obtained from Carlo Erba. All the reagents were analytical grade, and no further purification was applied. Millipore deionized water (resistivity; 18 MU cm) was used for all experiments. Fluorine doped tin oxide (FTO) coated glass substrates (R sh ; 20 U/sq., 2 mm in thickness) were used for electrodeposition experiments.
5
Graphene Oxide Synthesis and Characterization
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Graphite flakes were purchased from Ashbury Inc. (Charlottesville, VA, USA), Sulphuric acid (H2SO4, 98%), potassium permanganate (KMnO4, 99.9%), hydrogen peroxide (H2O2, 30%), iron (II) sulphate (FeSO4·7H2O, 99.5%) and ammonium hydroxide (NH4OH, 25%) were purchased from Systerm (Shah Alam, Selangor). 3-Hydroxytyraminium chloride (dopamine, DA) and L-(+)-ascorbic acid (AA) were purchased from Merck (New York, NL, USA). Potassium hexacyanoferrate (III) (K3[Fe(CN)6]) was purchased from Sigma Aldrich (St. Louis, MO, USA). All other chemicals were of analytical grade and used without further purification. Stock solutions of DA and AA were freshly prepared using doubly-distilled (DI) water and used for electrochemical studies.
6
Analytical Quantification of Antibiotics
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All chemicals
were of analytical grade. Oxytetracycline (OTC), tetracycline (TET),
chlortetracycline (CTC), doxycycline (DOX), amoxicillin (AMOX), ciprofloxacin
(CIP), and norfloxacin (NOR) were obtained from Sigma-Aldrich (Japan). N,N-dimethylformamide (DMF) was acquired
from Sigma-Aldrich (Japan). Methanol was obtained from RCI Labscan
(Thailand). Disodium hydrogen phosphate dihydrate and sodium dihydrogen
phosphate dihydrate were obtained from QRëc (New Zealand).
Potassium hexacyanoferrate(III) (K3Fe(CN)6)
was obtained from Sigma-Aldrich (USA and Spain).
were of analytical grade. Oxytetracycline (OTC), tetracycline (TET),
chlortetracycline (CTC), doxycycline (DOX), amoxicillin (AMOX), ciprofloxacin
(CIP), and norfloxacin (NOR) were obtained from Sigma-Aldrich (Japan). N,N-dimethylformamide (DMF) was acquired
from Sigma-Aldrich (Japan). Methanol was obtained from RCI Labscan
(Thailand). Disodium hydrogen phosphate dihydrate and sodium dihydrogen
phosphate dihydrate were obtained from QRëc (New Zealand).
Potassium hexacyanoferrate(III) (K3Fe(CN)6)
was obtained from Sigma-Aldrich (USA and Spain).
7
Synthesis and Characterization of PMMA
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Chemicals such as N, N-dimethylformamide (DMF, >99.8%), poly(methyl methacrylate) (PMMA, average molecular weight of 350,000 g/mol), iron(III) chloride (FeCl3, 97%), potassium hexacyanoferrate(III) (K3Fe(CN)6, ≥99%), caesium standard for ICP and barium, strontium, and rubidium standard for ICP were purchased from Sigma Aldrich Chemical Co. (Milwaukee, WI, USA). Ammonia solutions and hydrochloric acid (HCl) were purchased from Samchun Chemical (Seoul, Korea). All chemicals were used as received without further purification.
8
Synthesis and Characterization of Chitosan-PVA Hydrogel
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All chemicals were of analytical grade and used without further purification. d(+)Glucose, sorbitol, yeast extract (for use in microbial growth medium), agar powder bacteriological, chitosan (low molecular weight), poly(vinyl alcohol) (molecular weight, 1 × 105–1.1 × 105 AWU), N-vinylpyrrolidone, nitric acid, potassium hexacyanoferrate (III) (K3[Fe(CN)6]), 2,6-dichlorophenolindophenol (2,6-DCPIP), sodium hydrophosphate, sodium dihydrogen phosphate, and sodium dithionite were purchased from Sigma–Aldrich Chemicals (Steinheim, Germany). All aqueous solutions were prepared using water purified and deionized (18 MΩ) with an Aqualab AL-1 Double system (Aqualab Ltd., Moscow, Russia). PVA was modified with N-vinylpyrrolidone according to [20 (link)]. Multi-walled carbon nanotubes were kindly provided by Nanotech Center Ltd. (Tambov, Russia).
9
Electrochemical Detection of Cytosine-Rich Aptamer
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The cytosine-rich oligonucleotide aptamer (MB-CRO), with the sequence 5′-Methylene Blue-CCCCCCCCCCCCCCCCCCCCCCCC-3′ (24C) [65 (link)], was sourced from BIONEER Corporation (Daejeon, Republic of Korea). Graphite powder, phosphate buffer saline (PBS), Tris(hydroxymethyl)aminomethane hydrochloride (Tris-HCl), and potassium hexacyanoferrate (III) (K3[Fe(CN)6]) were procured from Sigma-Aldrich, Inc. (St. Louis, MO, USA). All chemicals employed were of analytical grade and utilized as received, without any further purification. The aqueous solutions were prepared using deionized water with a resistivity greater than 18 MΩcm, produced by a Millipore water purification system (MilliQ, Millipore Korea, Co., Ltd., Seoul, Republic of Korea).
10
Synthesis and Characterization of Amino Acid Nanoparticles
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All the amino acids including L-tyrosine, L-glycine, and L-cystine were purchased from Merck and employed as obtained. Silver nitrate (AgNO3), sodium borohydride (NaBH4), iron (III) chloride, potassium hexacyanoferrate (III) (K3Fe (CN)6), polyvinylpyrrolidone (PVP) (average molecular weight 40,000), pyrogallol, trisodium citrate (Na3Cit.2H2O), trichloroacetic acid (Cl3CCOOH), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma-Aldrich, USA. Phenyl methyl sulfonyl fluoride (PMSF) (purity > 99%), reduced glutathione (purity ≥ 98.0%), 2-mercapto-ethanol (purity ≥ 99.0%), and all other reagents were obtained from quality vendors and utilized as provided.
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