Cfpac 1

Four human pancreatic cancer cell lines (MiaPaCa-2, BxPC-3, CFPAC-1 and PANC-1) and a normal human pancreatic ductal cell line (HPNE) were purchased from the Shanghai Cell Bank (Shanghai, China). Cells stably overexpressing YY1 or with YY1 knockdown had already been transfected into the BxPC-3 and PANC-1 cell lines (BxPC-3-YY1, BxPC-3-YY1 shRNA, PANC-1-YY1, PANC-1-YY1 shRNA). The corresponding control cell lines (BxPC-3-Vector, BxPC-3-Scramble shRNA, PANC-1-Vector, PANC-1-Scramble shRNA) had also already been prepared.^{21 (link)}As described previously, PDAC cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% foetal calf serum (FBS) (Wisent Inc., Montreal, Qc, Canada), 10 mM HEPES (Sigma, St. Louis, MO, USA), 2 mM L-glutamine (Sigma), 1 mM pyruvate sodium (Sigma), 100 U/ml penicillin (Life Technologies), and 100 μg/ml streptomycin (Life Technologies). HPNE cells were grown in keratinocyte serum-free medium supplemented with epidermal growth factor and bovine pituitary extract at 37 °C in a humidified atmosphere containing 95% air and 5% CO₂^{10 (link)}

Three human PDAC cell lines (PANC-1, CFPAC-1, MIAPACA-2) and a normal human pancreatic ductal cell line (HPNE) were purchased from Shanghai Cell Bank (Shanghai, China). PDAC cell lines were cultured in Dulbecco’s modified medium (Wisent, Canada) supplemented with 10% fetal calf serum (Wisent, Canada). The HPNE cell line was grown in keratinocyte serum-free medium supplemented with epidermal growth factor and bovine pituitary extract according to the recommendations of the ATCC.

Five human pancreatic ductal adenocarcinoma (PDAC) cell lines (PANC-1, BxPC-3, AsPC-1, CFPAC-1, and MIA-PaCa-2) and a normal human pancreatic ductal cell line (hTERT-HPNE) were purchased from the Shanghai Cell Bank (Shanghai, China). According to the recommendation of ATCC, both BxPC-3 and AsPC-1 cell lines were cultured in RPMI­1640 Medium (Life Technologies; Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Life Technologies; Carlsbad, CA, USA) and 1% penicillin-streptomycin (HyClone, USA). However, PANC-1, CFPAC-1, and MIA-PaCa-2 were grown in Dulbecco's modified Eagle's medium (DMEM) (Life Technologies; Carlsbad, CA, USA) containing 10% FBS and 1% penicillin-streptomycin (HyClone, USA). In addition, the hTERT-HPNE cell line was maintained in DMEM, supplemented with 5% FBS, 10 ng/ml human recombinant epidermal growth factor (EGF), 5.5 mMD glucose (1 g/L), and 750 ng/ml puromycin. All cell lines were routinely cultured in a humidified incubator with 5% CO₂ at 37°C.