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2 protocols using escherichia coli cicc 20658

1

Tuna Skin Bioactive Compound Extraction

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Tuna skin was obtained from Ningbo Today Food Co. Ltd. (Zhejiang, China). Acetic acid, pepsin (1800 U/g), sodium chloride, chitosan, glycerol, sodium bromide, and LB medium powder were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Escherichia coli CICC 20658, Bacillus subtilis CICC 10732, Staphylococcus aureus CICC 10201, and Listeria monocytogenes ATCC 19111 were ordered from China Center of Industrial Culture Collection (Beijing, China). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), GA, TA, and EA were purchased from Sigma Company (St. Louis, MO, USA). All reagents used in the experiments were of analytical grade.
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2

Extraction and Characterization of Tuna Skin

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Tuna skin was supplied from Ningbo Today Food Co. Ltd. (Zhejiang, China). Glacial acetic acid, pepsin, sodium chloride, chitosan, glycerol, sodium bromide and LB medium were obtained from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Escherichia coli CICC 20658, Bacillus subtilis CICC 10732, Staphylococcus aureus CICC 10,201 and Listeria monocytogenes ATCC 19,111 were ordered from China Center of Industrial Culture Collection (Beijing, China). 1,1-diphenyl-2-picryl-hydrazyl (DPPH) was purchased from Sigma Company (St. Louis, MO, USA). All reagents were of analytical grade.
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