GC–MS measurements were conducted following the method reported in previous studies [24 (link)], with minor modifications, using an Agilent 7890 gas chromatography system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent 5975C series mass spectrometer. The volatile compounds were isolated with a DB-WAX fused silica capillary column (30 m × 320 μm i.d. × 0.25 μm; Agilent Technologies). Helium (purity ≥99.999%) was used as the carrier gas at a rate of 1.0 mL/min constant flow. The oven temperature was held at 40 °C for 3 min, ramped at a rate of 5 °C/min to 120 °C, followed by ramping to 200 °C at a rate of 10 °C/min, and held for 5 min. MS was carried out in an electron impact mode of 70 eV with a scan range of 45–550 m/z.
Agilent 5975c series mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 5975C series mass spectrometer is a high-performance analytical instrument designed for the identification and quantification of chemical compounds. It utilizes electron ionization (EI) and chemical ionization (CI) techniques to generate and analyze molecular ions, providing detailed information about the structure and composition of the sample. The 5975C series is capable of performing accurate and sensitive mass analysis across a wide mass range, making it a versatile tool for a variety of applications in analytical chemistry and scientific research.
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2 protocols using agilent 5975c series mass spectrometer
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GC-MS Analysis of Volatile Compounds
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GC-MS Analysis of Volatile Compounds
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GC-MS analysis was carried out according to the method described by Heo et al. [14 (link)] using an Agilent 7890 gas chromatography system (Agilent Technologies, Santa Clara, CA, USA) equipped with an Agilent 5975C series mass spectrometer. The volatile compounds were isolated with HP-INNOWAX (30 m × 0.25 mm i.d. × 0.25 μm; Agilent Technologies) fused silica capillary columns. The carrier gas was helium at a rate of 1 mL/min constant flow. The oven temperature was held at 40 °C for 10 min, ramped at the rate of 8 °C/min to 180 °C, followed by ramping to 280 °C at the rate of 10 °C/min for 10 min. Mass spectrometry was carried out at an electron impact mode of 70 eV with a scan range of m/z 33–550. The volatile compounds were identified on the basis of the matching of the mass spectra with those in the standard NIST10 database. The quantification of volatile compounds was performed using peak areas normalized with 3-octanone added to each sample as an internal standard.
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