2-deoxy-D-glucose (Cat. no. D8375), the RAD51 inhibitor B02 (Cat. no. SML0364), and fludarabine phosphate (Cat. no. 1272204) were obtained from Sigma-Aldrich. DIDS was obtained from ChemCruz (Cat. no. sc-203919).
Fludarabine phosphate
Manufactured by Merck Group
Sourced in Germany, United States
Fludarabine phosphate is a synthetic purine analog used as a laboratory reagent. It is a white or off-white crystalline powder. Fludarabine phosphate is soluble in water and is commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
2 deoxy d glucose, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Gadolinium chloride hexahydrate, by Merck Group (1 mentions)
Trisodium citrate, by Carl Roth (1 mentions)
N butanol, by Honeywell (1 mentions)
Dimethyl sulfoxide (dmso), by AppliChem (1 mentions)
Fr180204, by Merck Group (1 mentions)
Cryptotanshinone, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Calcein am, by Thermo Fisher Scientific (1 mentions)
Recombinant murine gm csf, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Lonza (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Red blood cell lysing buffer, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by InvivoGen (1 mentions)
Dimethyl fumarate, by Merck Group (1 mentions)
Poly da dt, by Merck Group (1 mentions)
4 protocols using fludarabine phosphate
1
Inhibition of Glycolysis and DNA Repair
2
Fluorescent Labeling Reagents for Nucleic Acid Visualization
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Sodium dodecylsulfate (SDS, ≥99.0%, Sigma-Aldrich, Germany), n-butanol (>99.5%, Riedel-de-Haën, Germany), α-tocopherol (97%, ABCR, Germany), trisodium citrate (99%, Carl Roth, Germany), gadolinium chloride hexahydrate (>99%, Sigma-Aldrich), irinotecan (98%, ABCR, Germany), sodium adenosine monophosphate (>99%, Sigma-Aldrich, Germany), DUT549P1-aadUTP (Dyomics, Germany), ATTO647N-aadUTP (≥95%, Jena Bioscience, Germany) and fludarabine phosphate (>99%, Sigma-Aldrich, Germany) were used as purchased.
3
Transcription Factor Inhibitors Modulate NK-Cell
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The following transcription factor inhibitors were used: fludarabine phosphate (Sigma Aldrich, St. Louis, MO, USA)—STAT1 inhibitor; FR 180204 (Sigma Aldrich, St. Louis, MO, USA)—ERK1/2 inhibitor; and cryptotanshinone (Sigma Aldrich, St. Louis, MO, USA)—STAT3 inhibitor. All substances were diluted to the appropriate concentrations in DMSO (AppliChem, Darmstadt, Germany).
Freshly isolated NK-cells at 0.5 × 106/mL concentrations were cultivated at 37 °C with (a) IL-2, 100 U/mL for the control samples, or (b) IL-2 (100 U/mL) + IL-21 (50 ng/mL) for the experimental samples. On day 3, the medium with cytokines was renewed, and inhibitors were added: fludarabine phosphate, 0.1, 1, and 10 μg/mL; FR 180204, 0.5, 5, and 50 μg/mL; and cryptotanshinone, 0.1, 1, and 10 μg/mL. After 36 h, NK cells were labeled with fluorescent antibodies, and marker expression was measured through the use of flow cytometry.
Freshly isolated NK-cells at 0.5 × 106/mL concentrations were cultivated at 37 °C with (a) IL-2, 100 U/mL for the control samples, or (b) IL-2 (100 U/mL) + IL-21 (50 ng/mL) for the experimental samples. On day 3, the medium with cytokines was renewed, and inhibitors were added: fludarabine phosphate, 0.1, 1, and 10 μg/mL; FR 180204, 0.5, 5, and 50 μg/mL; and cryptotanshinone, 0.1, 1, and 10 μg/mL. After 36 h, NK cells were labeled with fluorescent antibodies, and marker expression was measured through the use of flow cytometry.
4
Murine GM-CSF and IFNβ stimulation
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RPMI 1640 medium was from Lonza, FBS was from Atlanta Biologicals, glutamine and penicillin-streptomycin were from Gibco, and recombinant murine GM-CSF was from PeproTech. Murine recombinant (r) IFNβ was from Pbl Assay Science. Dimethyl fumarate, Poly (dAdT), Red Blood Cell Lysing Buffer, fludarabine phosphate, and 2-mercaptoethanol were from Sigma. LPS was from Invivogen. Calcein AM was from Invitrogen. GeneJuice was from Novagen. S.mansoni genomic DNA was from the Schistosomiasis Resource Center (NIH).
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