1100 series lc msd trap system

Manufactured by Agilent Technologies

The 1100 Series LC/MSD Trap system is a liquid chromatography mass spectrometry (LC/MS) instrument designed for analytical applications. It combines a liquid chromatography system with a mass spectrometer to enable the separation, identification, and quantification of chemical compounds in complex samples. The system provides accurate mass measurements and ion trap technology for high-sensitivity detection and structural analysis.

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Lab products found in correlation

Avance 3 700 mhz, by Bruker (2 mentions) Icap 6000 series spectrometer, by Thermo Fisher Scientific (1 mentions) Zorbax sb c18, by Agilent Technologies (1 mentions) Avance dpx 300 mhz, by Bruker (1 mentions) Spectrum one, by PerkinElmer (1 mentions)

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1100 series (251 citations) 1100 series system (40 citations) 1100 LC system (29 citations) LC: 1100 series (20 citations) 1100 LC/MSD (18 citations) 1100 series LC system (12 citations) LC/MSD system (8 citations) 1100 Series LC/MSD Trap (7 citations) LC-MSD 1100 series (3 citations) 1100 Series LC/MSD system (2 citations)

5 protocols using 1100 series lc msd trap system

Platinum(II) Oxalato Complex Synthesis

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Commercial reagent grade chemicals and solvents (Sigma-Aldrich, Milan, Italy) were used as received without further purification. One-dimensional (1D) ¹H-NMR, 1D ¹³C-NMR, and [¹H-¹³C] HSQC 2D NMR spectra were recorded on Bruker Avance III 700 MHz and Bruker Avance DPX 300 MHz instruments (Bruker Italia S.r.L., Milano, Italy). ¹H and ¹³C chemical shifts were referenced using the internal residual peak of the solvent (DMSO-d₆: 2.50 ppm for ¹H and 39.51 ppm for ¹³C; D₂O: 4.80 ppm for ¹H, Acetone-d₆: 2.05 ppm for ¹H and 29.92 for ¹³C, CD₃OD: 3.31 ppm for ¹H and 49.15 ppm for ¹³C). Electrospray ionisation mass spectrometry (ESI-MS) was performed with an electrospray interface and an ion trap mass spectrometer (1100 Series LC/MSD Trap system Agilent, Palo Alto, CA).
Inductively Coupled Plasma Atomic Emission analyses were performed with an ICP-AES ThermoScientific iCap 6000 Series spectrometer (ICAP 6300 with dual view, empowered by iTeva software; Thermo Scientific, Waltham, MA, USA).
[Pt(OXA)(DACHEX)] and PhICl₂ [10 (link)] were synthesized according to previously reported procedures.

Papadia P., Micoli K., Barbanente A., Ditaranto N., Hoeschele J.D., Natile G., Marzano C., Gandin V, & Margiotta N. (2020). Platinum(IV) Complexes of trans-1,2-diamino-4-cyclohexene: Prodrugs Affording an Oxaliplatin Analogue that Overcomes Cancer Resistance. International Journal of Molecular Sciences, 21(7), 2325.

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Extraction and LC-MS Analysis of Bacterial Metabolites

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After finishing the fermentation, the culture broth of wild type and mutant strains were extracted by equal volume of ethyl acetate. The supernatant of the ethyl acetate phase was concentrated by rotary evaporator under the reduced pressure and finally dissolved in methanol (400 μL) for the LC-MS analysis using the Agilent 1100 series LC/MSD Trap system. The conditions for the LC-MS analysis are as follows: 55-100% B (linear gradient, 0–25 min, solvent A is water containing 0.1% formic acid, solvent B is acetonitrile containing 0.1% formic acid), 100% B (26–30 min) at the flow rate of 0.3 mL/min with a reverse-phase column ZORBAX SB-C18 (Agilent, 5 μm, 150 mm × 4.6 mm). Figure
4B was recorded with the conditions: 35-95% B (linear gradient, 0–20 min), 100% B (21–25 min), 35%B (25–40 min) at the flow rate of 0.3 mL/min.

Du Y., Wang Y., Huang T., Tao M., Deng Z, & Lin S. (2014). Identification and characterization of the biosynthetic gene cluster of polyoxypeptin A, a potent apoptosis inducer. BMC Microbiology, 14, 30.

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Quantifying DNA Modifications via HPLC-MS

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DNA PT modifications were analyzed using HPLC/Mass Spectrometry (Agilent 1100 series LC/MSD Trap system or Agilent HPLC 1290-MS 6230 system) (8). Briefly, modified DNA was digested using nuclease P1 and alkaline phosphatase. Twenty microliters of reaction products were loaded on a YMCC18 reverse-phase column (250 × 4.6 mm, 5 μm) and detected at 254 nm. HPLC was carried out at a flow rate of 0.3 ml/min at room temperature. Buffer A consisted of 0.1% acetic acid in water, and buffer B consisted of 0.1% acetic acid in acetonitrile. The following HPLC gradient was employed: 1–13% buffer B for 30 min, 13–30% buffer B for another 15 min, followed by 30–1% buffer B for 5 min, and finally 1% buffer B for 15 min. The drying gas flow was 10 L/min, the nebulizer pressure was 30 psi, the drying gas temperature was 325 °C, and the capillary voltage was 3,200 V (using the positive detection mode and scanning between 50 and 1000 m/z for mass spectrometer analysis).

Yang Y., Xu G., Liang J., He Y., Xiong L., Li H., Bartlett D., Deng Z., Wang Z, & Xiao X. (2017). DNA Backbone Sulfur-Modification Expands Microbial Growth Range under Multiple Stresses by its anti-oxidation function. Scientific Reports, 7, 3516.

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Quantification of Endogenous Metabolites

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Production of 1 in feeding experiments, concentrations of endogenous 10 and 11, and the products of enzymatic reactions were quantified by HPLC-triple quadrupole/mass spectrometry (HPLC-QQQ/MS) (Agilent 1100 series LC/MSD Trap System), working in the Multiple Reaction Monitoring model by employment of an unique precursor ion-to-product ion for each analyte to achieve highest selectivity and sensitivity. The parameters of polarity, precursor ion, product ion, fragmentor, and collision energy for the related compounds were individually optimized and listed in Supplementary Table 10. HPLC method was the same as that of HPLC-TOF/MS.

Zhao Q., Luo Y., Zhang X., Kang Q., Zhang D., Zhang L., Bai L, & Deng Z. (2020). A severe leakage of intermediates to shunt products in acarbose biosynthesis. Nature Communications, 11, 1468.

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Characterization of Platinum Complexes

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Commercial reagent grade chemicals and solvents were used as received without further purification. 1 H-NMR, 195 Pt-NMR and [ 1 H-195 Pt] HSQC spectra were recorded on a Bruker Avance DPX 300 MHz instrument. [ 1 H- 13 C]-HSQC spectra were recorded on a Bruker Avance III 700 MHz instrument. 1 H and 13 C chemical shifts were referenced using the internal residual peak of the solvent (DMSO-d 6 : 2.50 ppm for 1 H and 39.51 ppm for 13 C). 195 Pt NMR spectra were referenced to K 2 PtCl 4 (external standard placed at -1620 ppm with respect to Na 2 [PtCl 6 ]). [17] Electrospray ionisation mass spectrometry (ESI-MS) was performed with an electrospray interface and an ion trap mass spectrometer (1100 Series LC/MSD Trap system Agilent, Palo Alto, CA).
Elemental analyses were carried out with an Eurovector EA 3000 CHN instrument. The Fourier transform infrared spectra were obtained on a Perkin Elmer Spectrum One instrument, using KBr pellets, in the range 4000-200 cm 1 . Intensities of the reported bands are described with s for strong, m for medium and w for weak; broad signals are additionally specified with letter b in front of these abbreviations.
Kiteplatin [18] and cis,trans,cis-[PtCl 2 (OH) 2 (cis-1,4-DACH)] [19] (compound 3) were prepared according to already reported procedures and the given formulation was in good agreement with analytical values (data not shown).

Margiotta N., Savino S., Marzano C., Pacifico C., Hoeschele J.D., Gandin V, & Natile G. (2016). Cytotoxicity-boosting of kiteplatin by Pt(IV) prodrugs with axial benzoate ligands. Journal of inorganic biochemistry, 160.

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