UV-visible spectroscopy was performed with an Agilent Hewlett-Packard 8453 diode-array spectrophotometer equipped with an Agilent 89090A thermostat. Absorbance and fluorescence measurements of 96 well plates were accomplished with a Perkin-Elmer HTS 7000 Bio Assay reader. Cells were counted using a Beckman Coulter Z-2 cell and particle counter. Cell viability and angiogenesis was monitored using a Nikon eclipse TS-100 inverted microscope. An Eppendorf thermocycler was used to prepare cDNA. Sequence detection was then carried out using 7300 Real-Time PCR System from Applied Biosystems. An iBlot® gel transfer device from Invitrogen, Carlsbad, CA was used for dry transfer in Western blots. DNA damage was assessed using a Comet Assay® Electrophoresis System (Trevigen Inc. Gaithersburg, MD).
89090a thermostat
Manufactured by Agilent Technologies
The Agilent 89090A thermostat is a precision temperature control device designed for laboratory applications. It provides accurate and stable temperature regulation to meet the demands of various experimental and testing requirements. The core function of the 89090A is to precisely maintain a desired temperature within a specified range.
Automatically generated - may contain errors
Lab products found in correlation
8453 diode array spectrophotometer, by Agilent Technologies (2 mentions)
8453 uv vis spectrometer, by Agilent Technologies (2 mentions)
Z2 cell and particle counter, by Beckman Coulter (1 mentions)
Iblot gel transfer device, by Thermo Fisher Scientific (1 mentions)
7300 real time pcr system, by Thermo Fisher Scientific (1 mentions)
Hts 7000 bio assay reader, by PerkinElmer (1 mentions)
Eclipse ts100 inverted microscope, by Nikon (1 mentions)
Thermocycler, by Eppendorf (1 mentions)
Maldi tof mass spectrometer, by AB Sciex (1 mentions)
Drx 500 instrument, by Bruker (1 mentions)
Synergy 2 microplate reader, by Agilent Technologies (1 mentions)
Apollo 4000 system, by World Precision Instruments (1 mentions)
Uv 3600 plus uv vis nir spectrophotometer, by Shimadzu (1 mentions)
Vario el elemental analyzer, by Elementar (1 mentions)
Vector 22 ftir spectrometer, by Bruker (1 mentions)
Apexiv ft icr mass spectrometer, by Bruker (1 mentions)
4 protocols using 89090a thermostat
1
Multimodal Analytical Techniques in Cellular Research
2
Spectroscopic Characterization of Organic Compounds
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Unless otherwise stated, all reactions were performed under an inert atmosphere of nitrogen. UV-vis spectra were recorded on an Agilent 8453 UV-vis spectrometer equipped with an Agilent 89090A thermostat (±0.1 °C) at 25 °C. IR spectra were recorded on a Spectrum Spotlight 200 FT-IR microscope. Mass spectra were recorded on a Bruker APEX IV FT-ICR mass spectrometer (ESI) or AB Sciex MALDI-TOF mass spectrometer. Simulated mass spectra were obtained from the website http://www.chemcalc.org . NMR spectra were recorded on a Bruker ARX400 400 MHz or AVANCE III 500 MHz NMR spectrophotometer. The HeLa cells were obtained from Peking University Health Science Center. For the optical measurements in liquid solution, spectroscopic-grade dimethyl sulfoxide was used as purchased from Alfa-Aesar. Anhydrous 1,2,4-trichlorobenzene (TCB), diisobutyl aluminium hydride (DIBAL-H), sarcosine, and polyformaldehyde were purchased from J&K Scientific and used as received. Anhydrous CH2Cl2 was distilled from calcium hydride and H2O was obtained from Milli-Q Integral.
3
Analytical Techniques for Comprehensive Characterization
4
Spectroscopic Characterization of Porphyrin Complexes
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UV-vis spectra were recorded using an Agilent 8453 UV-vis spectrometer equipped with an Agilent 89090A thermostat (±0.1 °C) at 25 °C. Near-IR absorption spectra were recorded using a Shimadzu UV-3600 Plus UV-Vis-NIR Spectrophotometer. Mass spectra were recorded using a Bruker APEX IV FT-ICR mass spectrometer (ESI-MS). Elemental analyses (C, H, N) were performed using an Elementar Analysensysteme GmbH vario EL Elemental Analyzer. NMR spectra were recorded using a Varian Mercury Plus 300 MHz spectrophotometer or Bruker ARX400 400 MHz spectrophotometer. IR spectra were recorded using a Bruker VECTOR22 FTIR spectrometer and KBr pellets. For the optical measurements in liquid solution, spectroscopic grade CD2Cl2 was purchased from Cambridge Isotope Laboratories, Inc. and used as received. Anhydrous CH2Cl2 was distilled from calcium hydride and 1,2,4-trichlorobenzene (TCB) was purchased from J&K Scientific. The β-octafluorinated porphyrin ligands34 and deuterated Kläui's ligand18b (D atom > 99%) were synthesized according to literature methods.
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