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The FLAGM2 is a monoclonal antibody that specifically recognizes the FLAG epitope, a short peptide sequence that can be used as a tag to identify and purify recombinant proteins. The FLAGM2 antibody is useful for the detection and immunopurification of FLAG-tagged proteins expressed in various biological systems.

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Lab products found in correlation

Rootletin, by Santa Cruz Biotechnology (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) Lamin b1, by Santa Cruz Biotechnology (1 mentions) Ripa lysis buffer, by Merck Group (1 mentions) Mouse anti actin, by Abcam (1 mentions) Mouse anti flag, by Merck Group (1 mentions)

2 protocols using flagm2

1

Antibody Sourcing and Verification

Cited 2 times
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Nes1HAA12 and Nes1QFA13 antibodies were raised as previously described (Chambliss et al., 2013 (link); Potter et al., 2017 (link); Razafsky et al., 2013 (link)). Rootletin, Lamin B1, FLAGM2 and α-tubulin antibodies were purchased from Santa Cruz (#SC-67824, #SC-6216) and Sigma-Aldrich (#F3165, #T6199), respectively.
Potter C., Razafsky D., Wozniak D., Casey M., Penrose S., Ge X., Mahjoub M.R, & Hodzic D. (2018). The KASH-containing isoform of Nesprin1 giant associates with ciliary rootlets of ependymal cells. Neurobiology of disease, 115, 82-91.
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2

Protein Extraction and Immunoblotting

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Proteins from mouse tissues and cultured HEK293 cells were prepared with the RIPA Lysis Buffer (Sigma). SDS-PAGE and immunoblot analysis were performed according to standard protocols. Antibodies used were mouse anti-FLAG (Sigma; FLAG M2), rabbit anti-ADRB1 (Santa Cruz; sc568) and mouse anti-actin (Abcam; ab6276). Band intensities were determined using Image J software (NIH). Actin was used as a loading control.
Shi G., Xing L., Wu D., Bhattacharyya B.J., Jones C.R., McMahon T., Chong S.Y., Chen J.A., Coppola G., Geschwind D., Krystal A., Ptáček L.J, & Fu Y.H. (2019). A rare mutation of β1-adrenergic receptor affects sleep/wake behaviors. Neuron, 103(6), 1044-1055.e7.
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