1,6-Hexanediamine (92%), N,N-bis(3-aminopropyl)methylamine (98%), chloroform (99%), sodium hydroxide (50 wt% aqueous solution) and benzyltriethylammonium chloride (TEBAC) were purchased from Wanqing Chemical Instrument Co., Ltd (Nanjing, P.R.C.). Paclitaxel, oxaliplatin, cisplatin (DDP), verapamil (VRP) and z-VAD-FMK were obtained from Selleck Chemicals (Houston, USA). Annexin V-FITC/PI double-staining kit was provided by KeyGen (Nanjing, P.R.C.). N-acetyl-L-cysteine (NAC), hemoglobin, Hoechst 33342, crystal violet, 4’,6-diamidino-2-phenylindole (DAPI), 3-amino-4-aminomethyl-2’,7’-difluorescein diacetate (DAF-FM DA) and 2’,7’-dichlorofluorescein (DCFH-DA) were provided by Beyotime Biotechnology (Nanjing, P.R.C.). Propidium iodide (PI), calf thymus DNA (ct-DNA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and ethidium bromide (EB) were purchased from Sigma-Aldrich (St. Louis, USA). γ-H2AX (phospho S139) antibody was obtained from Abcam (Cambridge Science Park, Cambridge, UK). Primary antibodies against specific for cleaved caspase-3, cleaved caspase-9, Bax, poly (ADP-ribose) polymerase (PARP), β-actin and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Cell Signaling Technology (Boston, USA).
Cisplatin ddp
Manufactured by Selleck Chemicals
Sourced in United States
Cisplatin (DDP) is a platinum-based chemotherapy medication used in the treatment of various types of cancer. It functions as a DNA cross-linking agent, interfering with cell division and leading to cell death. Cisplatin is commonly used in the treatment of solid tumors, including those found in the ovaries, testes, head, neck, and lungs.
Automatically generated - may contain errors
Lab products found in correlation
Oxaliplatin, by Selleck Chemicals (1 mentions)
Paclitaxel, by Selleck Chemicals (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
2 7 dichlorofluorescein dcfh da, by Beyotime (1 mentions)
γ h2ax phospho s139 antibody, by Abcam (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Z vad fmk, by Selleck Chemicals (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc pi double staining kit, by Keygen Biotech (1 mentions)
Crystal violet, by Beyotime (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
N acetyl l cysteine nac, by Beyotime (1 mentions)
3 amino 4 aminomethyl 2 7 difluorescein diacetate daf fm da, by Beyotime (1 mentions)
Anti c fos, by Cell Signaling Technology (1 mentions)
Tumor necrosis factor α tnf α, by Merck Group (1 mentions)
Anti ki67, by Cell Signaling Technology (1 mentions)
Anti nf κb, by Cell Signaling Technology (1 mentions)
Anti cd31, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
6 protocols using cisplatin ddp
1
Evaluation of Anticancer Agents' Cytotoxicity
2
ESCC Cell Lines and Antibody Acquisition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PT and Cisplatin (DDP) were purchased from Selleckchem (Houston, TX, USA). Tumor necrosis factor-α (TNF-α) was obtained from Sigma Aldrich (St. Louis, MO, USA). Anti-CD31, anti-Ki67, anti-c-Fos, anti-c-Jun, and anti-NF-κB antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-VEGF and anti-GAPDH antibodies were obtained from Abclonal Technology (Wuhan, Hubei, China). The human ESCC lines–Eca109, KYSE-510 and human esophageal epithelial cells Het-1A were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) were obtained from Xiangya Central Experiment Laboratory. The cells were cultured at 37°C in a 5% CO2 incubator using Roswell Park Memorial Institute‐1640 (RPMI‐1640, Basal Media, China) containing 10% fetal bovine serum (FBS, Biological Industries, Israel), 100 U/mL penicillin and 100 μg/mL streptomycin.
3
Cisplatin and Etoposide Dissolution Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Cisplatin (DDP) and Etoposide(VP16)was purchased from Selleck and dissolved in DMSO to a final concentration of 5 mg/ml and 20 mg/ml. The CDC7 inhibitor XL413 was purchased from Selleck and dissolved in ddH2O to a final concentration of 10 mM.
4
Establishing HCC Cell Line Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immortalized normal liver cell LO2 and human HCC cell lines including MSK-Hep1, SNU-475, SNU-423, HepG2, Huh7, Huh1, SNU-182, and Hep3B were purchased from the ATCC and cultured in DMEM high glucose (HyClone) supplemented with 10% fetal bovine serum (FBS), and the cells were maintained at 37 °C in 5% CO2 incubator. Cisplatin (DDP) was purchased from Selleck, and 30 μM was used to treat cells. Pan-Akt inhibitor GSK690693 was purchased from Selleck, and 10 nM was used to treat cells.
5
Optimizing Zebrafish-Based Anticancer Drug Screening
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Four chemotherapy drugs Gemcitabine (GEM, Selleck Chemicals S1714), Paclitaxel (PTX, Selleck Chemicals S1150), Irinotecan (CPT-11, Selleck Chemicals S2217) and Vinorelbine (NVB, Selleck Chemicals S4269) were first dissolved in DMSO as 10 mM stocks; Cisplatin (DDP, Selleck Chemicals S1166) was dissolved in deionized water as 10 mM stocks and Tegafur (TGO, Ab142578) was dissolved in deionized water as 50 mg/mL; they were further diluted in E3 media to a final working concentration of 40 μM, 30 nM, 7 μM, 5 μM, 13 μM, and 25 μg/mL. The maximal lethal doses were determined based on preliminary tests. In a preliminary test for each drug, 60 hpf zebrafish were raised in 6-well plates (6 fish per will) in solutions containing different step concentrations, the solution was changed every day, and their survival conditions were recorded until 120 hpf. The concentration at which all zebrafish survived without significant malformations (curving tail, pericardium edema, and necrosis tissues) or abnormal behaviors (over- or no response to tap) was regarded as the MTD. Afterwards, Gemcitabine (40 μM) and Paclitaxel (30 nM), Irinotecan (7μM), Vinorelbine (5 μM), Cisplatin (13 μM) and Tegafur (25 μg/mL) were used on zebrafish to assess their effects on zPDX model.
6
Protein Expression and Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All antibodies were purchased from Abcam (Cambridge, USA), including anti-GAPDH, anti-PI3K, anti-p-PI3K, anti-Akt, anti-p-Akt, anti-m-TOR, anti-p-m-TOR, anti-Bax, anti-bcl-2, anti-E-cadherin, anti-N-cadherin, anti-P-glycoprotein (P-gp) antibody (at 1:1000 dilution, respectively) and HRP-labelled goat anti-mouse IgGs (at 1:2000 dilution). Cisplatin (DDP) was purchased from Selleck Chemicals (Houston, USA). DMSO was obtained from Sigma (St. Louis, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!