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Pe annexin 5 apoptosis kit 1

Manufactured by BD
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The PE Annexin V Apoptosis Kit I is a laboratory equipment product designed for the detection and quantification of apoptosis. It utilizes the binding properties of Annexin V, a protein that has a high affinity for phosphatidylserine, which is exposed on the cell surface during apoptosis. The kit provides the necessary reagents to perform this analysis.

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Lab products found in correlation

24 wells, by Corning (1 mentions) Cd44 clone im7, by BioLegend (1 mentions) Cd3ε clone 145 2c11, by BioLegend (1 mentions) Cd8α clone 53 6, by BD (1 mentions) Cd62l clone mel 14, by BioLegend (1 mentions) Cd4 clone rm4 5, by BioLegend (1 mentions) Lsrfortessa, by BD (1 mentions) Facscanto 2, by BD (1 mentions)

Close spelling variants of this product

BD Pharmingen PE Annexin V Apoptosis Detection Kit I Annexin V-PE/7-AAD Apoptosis Detection Kit I Annexin V Phycoerythrin (PE) Apoptosis Detection Kit I FITC Annexin V: PE Apoptosis Detection Kit I BD Pharmigen PE Annexin V Apoptosis Detection Kit I PE Annexin V and 7-AAD Apoptosis Detection Kit I PE Annexin V Apoptosis Detection Kit I PE Annexin V Kit I Annexin V Apoptosis Kit Annexin V-PE

5 protocols using pe annexin 5 apoptosis kit 1

1

Apoptosis in Adipocyte Co-culture

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Cumulus cells (CCs) were seeded in six-well plate (105 cells/well) and cultured for 12 h. Cells were harvested 12 h after being co-cultured with pre-adipocytes/mature adipocytes or extracellular vesicles derived from pre-adipocytes/mature adipocytes. All the cells after treatment were collected and were detected by flow cytometry with the PE/Annexin-V Apoptosis Kit I (BD Biosciences, USA).
Zhou G., Gu Y., Zhou F., Zhang H., Zhang M., Zhang G., Wu L., Hua K, & Ding J. (2022). Adipocytes-Derived Extracellular Vesicle-miR-26b Promotes Apoptosis of Cumulus Cells and Induces Polycystic Ovary Syndrome. Frontiers in Endocrinology, 12, 789939.
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2

Flow Cytometry Analysis of Cell Markers

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For flow cytometry measuring cell surface markers, differentiated CD34+ cells were incubated for 30 minutes with PE-Cy5-conjugated anti-CD71 (BD Biosciences PharMingen). For flow cytometry in DBA patient-derived CD34+ cells, we followed the protocols described in Doulatov et al.(58 (link)). For Annexin V/7-AAD staining, PE Annexin V Apoptosis Kit I (BD Biosciences PharMingen) was used, following manufacturer instructions.
Taylor A.M., Macari E.R., Chan I.T., Blair M.C., Doulatov S., Vo L.T., Raiser D.M., Siva K., Basak A., Pirouz M., Shah A.N., McGrath K., Humphries J.M., Stillman E., Alter B.P., Calo E., Gregory R.I., Sankaran V.G., Flygare J., Ebert B.L., Zhou Y., Daley G.Q, & Zon L.I. (2020). Calmodulin inhibitors improve erythropoiesis in Diamond-Blackfan anemia. Science translational medicine, 12(566), eabb5831.
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3

Cell Viability Assessment in Microfluidics

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We performed short-term (0 h) and long-term (24 and 48 h) viability experiments on cells collected from the target outlet. The PE Annexin V Apoptosis Kit I (BD Biosciences, United States) was used to detect short-term cell viability. The kit contains two dyes, PE Annexin V and 7-Amino-Actinomycin (7-AAD), which can detect early apoptotic cells (PE Annexin positive, 7-AAD negative), late apoptotic cells or dead cells (PE Annexin positive and 7-AAD positive), and viable cells (PE Annexin and 7-AAD negative). Cell viability was calculated as the ratio of the number of viable cells to the total number of cells. For the long-term cell viability, the collected cells were cultured in 24 wells (Corning, United States) for further observation of the cell viability and morphology after going through the chip at 24 and 48 h. All cell viability tests were designed with the control groups.
Li B.W., Wei K., Liu Q.Q., Sun X.G., Su N., Li W.M., Shang M.Y., Li J.M., Liao D., Li J., Lu W.P., Deng S.L, & Huang Q. (2021). Enhanced Separation Efficiency and Purity of Circulating Tumor Cells Based on the Combined Effects of Double Sheath Fluids and Inertial Focusing. Frontiers in Bioengineering and Biotechnology, 9, 750444.
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4

Evaluating Cell Viability via Flow Cytometry

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To determine cell viability, cells were analysed by flow cytometry using the PE Annexin V Apoptosis Kit I (BD Bioscience, CA, USA), according to manufacturer’s instructions.
Vanders R.L., Hsu A., Gibson P.G., Murphy V.E, & Wark P.A. (2019). Nasal epithelial cells to assess in vitro immune responses to respiratory virus infection in pregnant women with asthma. Respiratory Research, 20, 259.
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5

Multiparameter Flow Cytometry Analysis

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All samples were analyzed on BD FACSCanto II or BD LSRFortessa cytometers, and data were analyzed using FlowJo software (FlowJo, LLC). The following antibodies/clones were used for cell analysis: CD3ε (clone 145-2C11, FITC, BioLegend; clone 145-2C11, APC, BioLegend), CD4 (clone RM4.5, FITC, BioLegend; clone RM4.5, eFluor 450, eBioscience; clone RM4.5, PerCP-Cy5.5, eBioscience), CD5 (clone 53-7.3, FITC, BD Biosciences), CD8α (clone 53-6.7, APC, BD Biosciences), CD24 (clone MI/69, FITC, BioLegend), CD25 (clone PC61, PE, Biolegend), CD44 (clone IM7, FITC, BioLegend), CD45.1/Ly5.1 (clone A20, eFluor 450, eBioscience), CD62L (clone MEL-14, PE, BioLegend), CD69 (clone H1.2F3, PE-Cy7, BioLegend), CD90.1/Thy1.1 (clone OX-7, PE, BioLegend), CTLA-4 (clone UC10-4B9, APC, eBioscience), Foxp3 (clone FJK-16S, APC, eBioscience), PD-1 (clone RMPI-30, PE-Cy7, BioLegend), TCRβ (clone H57-597, PerCP-Cy5.5, BioLegend; clone H57-597, FITC, BD Biosciences).
For measurement of apoptosis, the PE Annexin V Apoptosis Kit I (BD Pharmingen) was used according to manufacturer’s instructions.
Milam A.A., Bartleson J.M., Donermeyer D.L., Horvath S., Durai V., Raju S., Yu H., Redmann V., Zinselmeyer B., White J.M., Murphy K.M, & Allen P.M. (2018). Tuning T Cell Signaling Sensitivity Alters the Behavior of CD4+ T Cells During an Immune Response. Journal of immunology (Baltimore, Md. : 1950), 200(10), 3429-3437.
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