FTIR imaging acquisitions of whole hepatic samples were obtained using the Spectrum SpotlightTM 400 microscope coupled to a Spectrum One FTIR spectrometer (Perkin Elmer, Villebon sur Yvette, France). This device was equipped with a liquid nitrogen-cooled, 16-pixel line mercury cadmium telluride (MCT) detector for imaging. The pixel size used was 25 × 25 μm2. Spectra were recorded in the 4000–650 cm–1 range with 8 accumulations per pixel at a resolution of 4 cm−1. Prior to image acquisition, a background spectrum was recorded on a clean surface of the CaF2 window with 90 accumulations at 4 cm−1 spectral resolution and automatically removed from each pixel spectrum using the Spectrum Image software (Perkin Elmer, Waltham, MA, USA). In addition, an image of pure paraffin was collected at the periphery of the tissue with the same parameters and used for digital deparaffinisation in the extended multiplicative signal correction (EMSC) model.
Spectrumimage software
Manufactured by PerkinElmer
Sourced in United States
SpectrumIMAGE software is a specialized application developed by PerkinElmer for the analysis and visualization of infrared (IR) and Raman spectroscopic imaging data. The software provides essential tools for processing, analyzing, and interpreting the acquired spectral images, enabling researchers and analysts to extract valuable information from their samples.
Automatically generated - may contain errors
4 protocols using spectrumimage software
1
FTIR Imaging of Whole Hepatic Samples
2
FTIR Microscopy Mapping of Recycled Polymer Surfaces
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The FTIR microscopy mapping was performed using the FTIR PerkinElmer Spectrum 3 with the Spotlight 200i frontier microscope (PerkinElmer, Waltham, MA, USA). The mapping was performed on the surfaces of the breaks previously investigated with stereo microscopy. A mesh combination of 20 × 50 markers was determined with 100 µm spacing between them (as shown in Figure 2 ). The size of the aperture was set to 100 × 100 µm. The measurements were performed in ATR mode with a diamond ATR crystal using 18% of force. A total of 16 scans with a resolution of 4 cm−1 were acquired from each sample. All the spectra were recorded at room temperature in a wavenumber range between 4000 and 700 cm−1. The obtained results were analyzed using the SpectrumIMAGE software from PerkinElmer. For the r3L sample, the spectra obtained on the surface were compared with PP spectra, for the rEL sample with PA 6, and for the rDSM sample again with PP spectra.
3
FTIR Mapping and PCA Analysis
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FTIR analyses were performed using a Spotlight 400 microspectrometer (Perkin–Elmer, Waltham, MA). FTIR mapping was performed in Attenuated Total Refection (ATR) mode using the ATR-imaging accessory with a germanium crystal. The scanned area was 400 × 400 µm² (lateral resolution of 1.56 µm). The Mercury-Cadmium-Telluride (MCT) detector (Perkin–Elmer, Waltham, MA) covered the range 720–4000 cm−1 with a spectral resolution of 8 cm−1. Principal Component Analysis (PCA) calculation was applied to raw FTIR images using Spectrum IMAGE software (Perkin–Elmer).
4
ATR-FTIR Spectroscopy of Injection-Molded Samples
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The infrared spectra were recorded with a PerkinElmer Spectrum 3 ATR-FTIR spectrometer (PerkinElmer, Waltham, MA, USA) The GladiATR accessory, (PIKE Technologies, Madison, WC, USA) contained a monolithic diamond crystal. A total of 16 scans with a resolution of 4 cm−1 were acquired from each sample. All the spectra were recorded at room temperature in a wavenumber range between 4000 and 600 cm−1.
The FTIR microscopy mapping was performed using the FTIR PerkinElmer Spectrum 3 with the Spotlight 200i frontier microscope (PerkinElmer, Waltham, MA, USA). The mapping was performed on the surface of the injection-molded samples at sizes of 5 × 5 mm for each material produced. On the examined surface a mesh combination of 25 × 25 markers was determined with 100 µm spacing between them (as shown inFigure 1 ). The size of the aperture was set to 100 × 100 µm. The measurements were performed in ATR mode with a diamond ATR crystal using 18% of force. A total of 16 scans with a resolution of 4 cm−1 were acquired from each sample. All the spectra were recorded at room temperature in a wavenumber range between 4000 and 700 cm−1. The results obtained were analyzed using the SpectrumIMAGE software (version: R1.11.2.0016) from PerkinElmer. For samples r3L and rDSM, the spectra obtained on the surface were compared with the PP spectra, and for sample rEL with the PA 6 spectra.
The FTIR microscopy mapping was performed using the FTIR PerkinElmer Spectrum 3 with the Spotlight 200i frontier microscope (PerkinElmer, Waltham, MA, USA). The mapping was performed on the surface of the injection-molded samples at sizes of 5 × 5 mm for each material produced. On the examined surface a mesh combination of 25 × 25 markers was determined with 100 µm spacing between them (as shown in
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