We intraperitoneally injected 100 μL of luciferin substrate (15 mg/mL) (Biosynth). We performed optical imaging with the Lago optical imaging system (Spectral Instruments Imaging) 15 minutes later and analyzed the images with the Aura software from the same manufacturer.
Luciferin substrate
Manufactured by Biosynth
Sourced in United States
Luciferin substrate is a compound used in bioluminescence assays. It serves as a substrate for the enzyme luciferase, which catalyzes the oxidation of luciferin, resulting in the emission of light. The luciferin substrate is a key component in various applications that rely on light-producing reactions, such as cell-based assays and in vivo imaging.
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4 protocols using luciferin substrate
1
Bioluminescent Imaging of Intraperitoneal Luciferin
2
In Vivo Bioluminescence Imaging
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Mice to be imaged were anesthetized with 1.5% isoflurane through a nose cone. Luciferin substrate (1 mg/mL PBS; Biosynth, Itasca, IL, USA) was injected into the intraperitoneal cavity (100 μL/10 g body weight). 10 min after luciferin injection, luminescence was detected using an in vivo imaging system (IVIS Spectrum Pre-Clinical In Vivo Imaging System, PerkinElmer, Waltham, MA, USA) and associated software for quantitation. Luminescence images were acquired at exposure times of 60 s or less. Luminescence was quantified within regions of interest in units of photons/s per cm2.
3
In Vivo Bioluminescence Imaging
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Mice to be imaged were anesthetized with 1.5% isofluorane through a nose cone. Luciferin substrate (1 mg/mL PBS; Biosynth, Itasca, IL) was injected into the intraperitoneal cavity (100 μL/10 g body weight). 10 min after luciferin injection, luminescence was detected using an in vivo imaging system (IVIS Spectrum Pre-Clinical In Vivo Imaging System, PerkinElmer, Waltham, MA) and associated software for quantitation. Luminescence images were acquired at exposure times of 1 s or less. Luminescence was quantified within regions of interest in units of photons/s/cm2.
4
Bioluminescence Imaging Protocol
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One hundred microliters of luciferin substrate (15 mg/mL) (Biosynth) were intraperitoneally injected. Fifteen minutes later, optical imaging was performed using the Lago optical imaging system (Spectral Imaging Instruments). Analysis was conducted with the Aura Software from the same manufacturer.
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