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Instrument software v1

Manufactured by Thermo Fisher Scientific
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Sourced in United Kingdom

Instrument software v1.3.1 is a software application designed to control and operate laboratory instruments. It provides an interface for users to configure, monitor, and collect data from compatible instruments.

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Lab products found in correlation

High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (3 mentions) Rneasy plus micro kit, by Qiagen (2 mentions) Rneasy mini kit, by Qiagen (1 mentions) Quantstudio 3 real time pcr system, by Thermo Fisher Scientific (1 mentions) Powerup sybr green master mix, by Thermo Fisher Scientific (1 mentions) Nanodrop 2000, by Thermo Fisher Scientific (1 mentions)

3 protocols using instrument software v1

1

Real-Time PCR Analysis of Apoptosis Genes

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cDNA was synthesized from RNA isolated (RNeasy Plus Micro Kit, QIAGEN) from cells with a high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). cDNA was amplified with on a 6500 Fast Real-Time PCR System (Applied Biosciences, Warrington, United Kingdom) using Power SYBR Green PCR Master Mix (Applied Biosciences). Ct values were obtained in triplicate for each target including Nfkbia, Nfkb2, Myc, Rel, Irf4, Bcl2l1, Bcl2l11, Bcl2a1, Bcl2, Mcl1, Bak1, Bax, Bad, Bbc3, Bid, Pmaimp1, Ccne1, E2f3, Ubc, and B2M (See Table S1). Data were analyzed with the instrument software v1.3.1 (Applied Biosystems, Warrington, United Kingdom). All plots show relative expression based on difference between stimulated and unstimulated samples. For all plots mean ± 95% confidence interval is shown.
Berry C.T., Liu X., Myles A., Nandi S., Chen Y.H., Hershberg U., Brodsky I.E., Cancro M.P., Lengner C.J., May M.J, & Freedman B.D. (2020). BCR-Induced Ca2+ Signals Dynamically Tune Survival, Metabolic Reprogramming, and Proliferation of Naive B Cells. Cell reports, 31(2), 107474.
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2

Real-Time PCR Analysis of Apoptosis Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA was synthesized from RNA isolated (RNeasy Plus Micro Kit, QIAGEN) from cells with a high capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). cDNA was amplified with on a 6500 Fast Real-Time PCR System (Applied Biosciences, Warrington, United Kingdom) using Power SYBR Green PCR Master Mix (Applied Biosciences). Ct values were obtained in triplicate for each target including Nfkbia, Nfkb2, Myc, Rel, Irf4, Bcl2l1, Bcl2l11, Bcl2a1, Bcl2, Mcl1, Bak1, Bax, Bad, Bbc3, Bid, Pmaimp1, Ccne1, E2f3, Ubc, and B2M (See Table S1). Data were analyzed with the instrument software v1.3.1 (Applied Biosystems, Warrington, United Kingdom). All plots show relative expression based on difference between stimulated and unstimulated samples. For all plots mean ± 95% confidence interval is shown.
Berry C.T., Liu X., Myles A., Nandi S., Chen Y.H., Hershberg U., Brodsky I.E., Cancro M.P., Lengner C.J., May M.J, & Freedman B.D. (2020). BCR-Induced Ca2+ Signals Dynamically Tune Survival, Metabolic Reprogramming, and Proliferation of Naive B Cells. Cell reports, 31(2), 107474.
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3

Quantitative mRNA Expression Analysis

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Total mRNA was isolated from A20 cells and primary B lymphocytes using an RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions. RNA concentrations were measured using a NanoDrop 2000 (Thermo Scientific) and 1 µg of DNAse I treated RNA was used with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). cDNA was amplified on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) using PowerUp SYBR Green Master Mix (Applied Biosystems). PCR amplification was performed by initial activation for 2  min at 50°C, followed by a 95°C 2 min melt step. The initial melt steps were then followed by 40 cycles of 95°C for 15  s, 60°C for 15 s, and 72°C for 30 s. Data were analyzed with the instrument software v1.3.1 (Applied Biosystems) and analysis of each target was carried out using the comparative Ct method.
Emrich S.M., Yoast R.E., Zhang X., Fike A.J., Wang Y.H., Bricker K.N., Tao A.Y., Xin P., Walter V., Johnson M.T., Pathak T., Straub A.C., Feske S., Rahman Z.S, & Trebak M. (2023). Orai3 and Orai1 mediate CRAC channel function and metabolic reprogramming in B cells. eLife, 12, e84708.
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