Anti myod1

Manufactured by Cell Signaling Technology

Anti-MYOD1 is a research-use only antibody that recognizes the MYOD1 protein. MYOD1 is a transcription factor that plays a key role in skeletal muscle cell differentiation. The Anti-MYOD1 antibody can be used to detect and study MYOD1 expression in various experimental systems.

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MYOD1 (5 citations)

2 protocols using anti myod1

Quantitative Western Blot Analysis of Protein Markers

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Cell lysates were prepared in RIPA buffer supplemented with protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). Protein lysates (25–35 μg/lane), as determined by BCA protein assay (Life Technologies), were then separated by SDS-PAGE (NuPAGE; 4%–12% Bis-Tris gels; Invitrogen) and transferred on to a nitrocellulose membrane that was probed with anti-FGFR4 (Cell Signaling Technology, cat. #8562), anti-IGF-1Rβ (Cell Signaling Technology, cat. #9750), anti-MYOD1 (Cell Signaling Technology, cat. # 13812), anti-FOXO1 (Cell Signaling Technology, cat. #2880) and anti-β-Actin (Cell Signaling Technology, cat. #4967) primary antibodies followed by anti-rabbit IgG, HRP-linked secondary antibody (Cell Signaling Technology, cat. #7074) before detection using an iBright CL1000 imaging system (Thermo Fisher Scientific, MA, USA). iBright analysis software was used for quantification of the intensity of bands of interest.

Darvishi E., Slemmons K., Wan Z., Mitra S., Hou X., Hugues Parmentier J., Eddie Loh Y.H, & Helman L.J. (2020). Molecular mechanisms of Guadecitabine induced FGFR4 down regulation in alveolar rhabdomyosarcomas. Neoplasia (New York, N.Y.), 22(7), 274-282.

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Comprehensive Immunoblotting Antibody Panel

Cited 1 time

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The following commercially available primary antibodies were used for immunoblotting: anti-IRS2 (Cell Signaling Technologies, 4502) 1:750; anti-Cdh1/Fzr1 (Sigma Aldrich, CC43) 1:500; anti-anillin (a gift from Christine Field (21 (link))) 1:1000; anti-Aurora B (Bethyl, A300-431) 1:1000; anti-Eg5 (Cell Signaling Technologies, 4203) 1:1000; anti-Top2A (Cell Signaling Technologies, 12286) 1:1000; anti-TK1 (Cell Signaling Technologies, 8960) 1:1000; anti-Mps1 (Abcam, ab11108), 1:1000); anti-APC3 (BD Transduction Laboratories, 610455) 1:500; anti-cyclin B1 (Santa Cruz Biotechnology, sc-752) 1:500; anti-Cdc20 (Santa Cruz Biotechnology, sc-8358) 1:500; anti-c-Myc (9E10, Santa Cruz Biotechnology, sc-40) 1:1000; anti-HA-peroxidase (Sigma Aldrich), 1:1500; anti-cyclin A2 (Santa Cruz Biotechnology, sc-596) 1:500; anti-IRS1 (Cell Signaling Technologies, 2382) 1:750; anti-MyoD1 (Cell Signaling Technologies, 13812) 1:750; anti-GAPDH (Abcam, ab8245) 1:2000; anti-α tubulin (Abcam, ab7291 and Santa Cruz Biotechnology, sc-8035) 1:1000 for both; anti-vinculin (Santa Cruz Biotechnology, sc-73614) 1:2000. Secondary antibodies used: anti-rabbit IgG-HRP (GE Healthcare, NA934) and anti-mouse IgG-HRP (GE Healthcare, NA931V), both at 1:3000 dilutions.

Manohar S., Yu Q., Gygi S.P, & King R.W. (2020). The Insulin Receptor Adaptor IRS2 is an APC/C Substrate That Promotes Cell Cycle Protein Expression and a Robust Spindle Assembly Checkpoint. Molecular & Cellular Proteomics : MCP, 19(9), 1450-1467.

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