Peroxidase conjugated anti rabbit and anti mouse secondary antibodies

Rat anti-E-cadherin antibody (ECCD-2, 13-1900) and rabbit anti-ZO-1 antibody (61-7300) were from Thermo Fisher Scientific. Alexa Fluor-647-conjugated phalloidin (Invitrogen, Waltham, Massachusetts, USA) was used at 1.0 U/ml. Alexa Fluor-568- and Alexa Fluor-647-conjugated secondary antibodies were from Life Technologies (Waltham, Massachusetts, USA). Rabbit anti-FBP17 antibody [20 (link)] was gifted by Dr Toshiki Itoh. Mouse anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (6C5, MAB374) was from Millipore (Darmstadt, Germany). For immunofluorescence, primary antibodies were used at 1:100, and all secondary antibodies were used at 1:200. For western blotting, peroxidase-conjugated anti-rabbit and anti-mouse secondary antibodies were from GE Healthcare (Chicago, Illinois, USA) and Jackson ImmunoResearch (Burlington, Massachusetts, USA), respectively.

Rabbit anti-FBP17 antibody was generated in a previous study (Itoh et al., 2005 (link)). Rabbit anti-GST antibody (sc-459) was purchased from Santa Cruz Biotechnology. Rabbit anti-DsRed antibody (632496) from Clontech and Rabbit anti-RFP antibody (PM005) from MBL were used for western blotting and immuno-EM, respectively. Mouse anti-GAPDH antibody (6C5, MAB374) was from Millipore. Rat anti-E-cadherin antibody (ECCD-2, 13-1900) and rabbit anti-ZO-1 antibody (61-7300) were from Thermo Fisher Scientific. Mouse anti-Filamin antibody (F6682) was from Sigma. Rabbit anti-phospho-Myosin light chain 2 (Thr18/Ser19) antibody (E2J8F, 95777) was from Cell Signaling Technology. Alexa Fluor-568- or -647-conjugated phalloidin (Invitrogen) was used at 1.0 U/ml. Alexa Fluor-568- and -647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. For immunofluorescence, primary antibodies were used at 1:100, except for GST antibody that was used at 1:50, and all secondary antibodies were used at 1:200. For western blotting, peroxidase-conjugated anti-rabbit and anti-mouse secondary antibodies were from GE Healthcare and Jackson ImmunoReseach, respectively. The selective Cdc42 inhibitor ML141 (20 μM) and the antioxidant Trolox (1 mM) were from Tocris Bioscience and Cayman Chemical, respectively.