An Annexin V-Fluorescein Isothiocyanate (FITC)/Propidium Iodide (PI) Staining kit (BD Biosciences, Franklin Lakes, NJ, USA) was used to detect apoptotic cells. Cells were harvested 48 h following transfection and resuspended in 1X binding buffer at a concentration of 1×106 cells/ml. Subsequently, cells were stained with 5 µl Annexin V-FITC and 5 µl PI for 15 min at room temperature in the dark. Cells were acquired using a BD Accurri™ C6 flow cytometer (BD Biosciences) and data was analyzed using FlowJo software version 7.6.2 (Tree Star, Inc., Ashland, OR USA).
Accurri c6 flow cytometer
Manufactured by BD
Sourced in United States, Japan
The BD Accurri™ C6 flow cytometer is a compact and versatile instrument designed for analytical flow cytometry. It features a solid-state diode laser and up to 4 fluorescence detection channels, enabling the analysis of multiple parameters in a single sample. The BD Accurri™ C6 is capable of providing reliable and reproducible data for a variety of applications.
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Lab products found in correlation
2 protocols using accurri c6 flow cytometer
1
Apoptosis Detection by Annexin V-FITC/PI Assay
2
Measuring Mitochondrial Membrane Potential
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To evaluate the mitochondrial membrane potential (MMP), a lipophilic cationic probe 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazol-carbocyanine iodide (JC-1, 5 mg l−1, Biotime) was added to cell cultures for 30 min at 37 °C and fluorescence was measured by using a fluorescent microscope (Olympus, Tokyo, Japan) or a BD Accurri C6 Flow Cytometer. In all, 10 μM CCCP (carbonyl cyanide 3-chlorophenylhydrazone, Beyotime)-pretreated groups were used as positive control. The experiment was repeated at least three times.
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