The human PDAC cell lines PANC-1, BxPC-3 and HEK293T cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and were cultured in DMEM (GIBCO, USA) for PANC-1 and HEK293T cells or RPMI 1640 (GIBCO, USA) for BxPC-3 cells. Cell lines were maintained in culture supplemented with 10% FBS (GIBCO, USA) and 1% penicillin/streptomycin (Thermo, USA) at 37 °C with 5% CO2 in a humidified incubator (Thermo, USA).
The following antibodies and reagents were used: anti-RIOK3 (A305-601A-T, Thermo, USA), anti-FAK (71433S, CST, USA), anti-Phospho-FAK (Tyr397) (8556, CST, USA), anti-Phospho-FAK (Tyr925) (3284, CST, USA), anti-Phospho-FAK (Tyr925) (AF3619, Affinity Biosciences, USA), anti-Integrin β1 (9699, CST, USA), anti-Integrin β4 (14803, CST, USA), anti-Integrin β5 (3629, CST, USA), anti-MYC Tag (60003-2-Ig, Proteintech, China), anti-Vinculin (SC-73614, Santa Cruz Biotechnology, USA) and cycloheximide (CHX Inalco Spa Milano Italy).
The following antibodies and reagents were used: anti-RIOK3 (A305-601A-T, Thermo, USA), anti-FAK (71433S, CST, USA), anti-Phospho-FAK (Tyr397) (8556, CST, USA), anti-Phospho-FAK (Tyr925) (3284, CST, USA), anti-Phospho-FAK (Tyr925) (AF3619, Affinity Biosciences, USA), anti-Integrin β1 (9699, CST, USA), anti-Integrin β4 (14803, CST, USA), anti-Integrin β5 (3629, CST, USA), anti-MYC Tag (60003-2-Ig, Proteintech, China), anti-Vinculin (SC-73614, Santa Cruz Biotechnology, USA) and cycloheximide (CHX Inalco Spa Milano Italy).