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2 protocols using roq c

1

Quantification of Mycotoxin Standards

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The individual mycotoxin solid calibration standards (1 mg) of DON, DON-3-glucoside, 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), DOM-1, ZEN, α-ZEL, β-ZEL, T-2, T-2 triol, HT-2, DAS, AOH, AME, NIV, OTA, OTα, AFB1, AFB2, AFG1, AFG2, AFM1, FB1, FB2, FB3 and ROQ-C and internal standards (isotope-labelled DON, ZEN, AFB1 and FB1) were obtained from Sigma-Aldrich (Bornem, Belgium). All mycotoxin solid standards were dissolved in methanol (1 mg/mL) and were storable for a minimum of 1 year at −18 °C [48 (link)]. The mycotoxin working solutions and IS were prepared in methanol, and stored at −18 °C. Water was obtained from an Aurim® Pro water system from Sartorius (Brussels, Belgium). Disinfectol® (denaturated ethanol with 5% ether) was supplied by Chem-Lab (Zedelgem, Belgium). Methanol (LC-MS grade) was purchased from BioSolve (Valkenswaard, The Netherlands), while acetonitrile (Analar Normapur) and ammonium acetate were obtained from VWR International (Zaventem, Belgium). Acetic acid (glacial, 100%) and formic acid (98‒100%) were supplied by Merck (Darmstadt, Germany). VAMS devices (MitraTM) were obtained from Neoteryx (Torrance, CA, USA).
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2

Mycotoxin Standards Preparation Protocol

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The standards of DAS, DON, DON-3-Glc, 3ADON, 15ADON, F-X, FB1, FB2, FB3, NEO, NIV, T-2, HT-2, ZEN, AFB1, AFB2, AFG1, AFG2, STE, OTA, ROQ-C, AOH and AME were obtained from Sigma Aldrich (Bornem, Belgium). Internal standard deepoxydeoxynivalenol (DOM-1) was obtained from Romer Lab (Getzersdorf, Austria). All mycotoxin solid standards were dissolved in methanol (1 mg/mL) and stored at -18 °C.
Water was obtained from a Milli-Q ® SP Reagent water system from Millipore Corp.
(Brussels, Belgium). Disinfectol® (denaturated ethanol with 5% ether) was supplied by Chem-Lab (Zedelgem, Belgium). Methanol (LCMS grade) was purchased from BioSolve (Valkenswaard, the Netherlands), while acetonitrile (Analar Normapur), was obtained from VWR International (Zaventem, Belgium). Acetic acid (glacial, 100%) was supplied by Merck (Darmstadt, Germany). Magnesium sulphate and sodium chloride were purchased from Fischer Scientific (New Jersey, USA).
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